公开(公告)号：US20070163002A1

公开(公告)日：2007-07-12

申请号：US10757909

申请日：2004-01-15

申请人： Lorin DeBonte ,  Zhegong Fan ,  Guo-Hua Miao

发明人： Lorin DeBonte ,  Zhegong Fan ,  Guo-Hua Miao

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N9/04 ,  C12N15/82

CPC分类号： C12N9/0083 ,  C07C53/126 ,  C07C57/02 ,  C07C57/03 ,  C12N15/8247

摘要： Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

摘要翻译： 提供具有高油酸的种子，植物和油; 低亚油酸; 低亚油酸加亚麻酸; 和有利的功能或营养特性。 公开了在δ-12或δ-15脂肪酸去饱和酶基因中含有突变的植物。 优选的植物是油菜籽和向日葵植物。 携带这种突变基因的植物已经改变了种子中的脂肪酸组成。 在一个实施方案中，植物在具有保守基序His-Xaa-Xaa-Xaa-His的区域中含有突变，其在Δ-12和δ-15脂肪酸去饱和酶中发现。 优选的基序具有序列His-Glu-Cys-Gly-His。 该基序中优选的突变具有氨基酸序列His-Lys-Cys-Gly-His。 公开了包含突变体Δ-12或δ-15脂肪酸去饱和酶基因序列的核酸片段。

公开(公告)号：US20060137040A1

公开(公告)日：2006-06-22

申请号：US11340318

申请日：2006-01-26

申请人： Lorin DeBonte ,  William Hitz

发明人： Lorin DeBonte ,  William Hitz

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/82 ,  C11B1/00

CPC分类号： C12N9/0083 ,  A01H5/10 ,  A23D9/00 ,  C11B1/10 ,  C12N15/8218 ,  C12N15/8247

摘要： An endogenous oil extracted from Brassica seeds is disclosed that contains, after crushing and extraction, greater than 86% oleic acid and less than 2.5% α-linolenic acid. The oil also contains less than 7% linoleic acid. The Brassica seeds are produced by plants that contain seed-specific inhibition of microsomal oleate desaturase and microsomal linoleate desaturase gene expression. Such inhibition can be created by cosuppression or antisense technology. Such an oil has a very high oxidative stability in the absence of added antioxidants.

公开(公告)号：US20050150006A1

公开(公告)日：2005-07-07

申请号：US11070678

申请日：2005-03-02

申请人： Dharma Kodali ,  Zhegong Fan ,  Lorin DeBonte

发明人： Dharma Kodali ,  Zhegong Fan ,  Lorin DeBonte

IPC分类号： A01H5/00 ,  A01H5/10 ,  C10M101/04 ,  C10N30/02 ,  C10N30/06 ,  C10N30/10 ,  C10N30/12 ,  C10N30/18 ,  C10N40/08 ,  C11B1/00 ,  C11B1/04 ,  C11B5/00 ,  C11C3/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C12R1/91 ,  A01H1/00

CPC分类号： C10M101/04 ,  A01H5/10 ,  C10M2207/40 ,  C10M2207/404 ,  C10N2240/08 ,  C11B1/00 ,  C12N15/8247

摘要： A triacylglycerol containing oil having a 1,3-dierucoyl 2-oleoyl glycerol (EOE) content of at least about 50% is described. Plants for producing an oil having a high EOE content are also described.

摘要翻译： 描述了含有1,3-二琥珀酰基2-油酰甘油（EOE）含量为至少约50％的含三酰基甘油的油。 还描述了用于生产具有高EOE含量的油的植物。

公开(公告)号：US20070028328A1

公开(公告)日：2007-02-01

申请号：US11523383

申请日：2006-09-18

申请人： Richard Brogie ,  Lorin Debonte ,  William Hitz ,  Guo-Hua Miao ,  Robert Reiter

发明人： Richard Brogie ,  Lorin Debonte ,  William Hitz ,  Guo-Hua Miao ,  Robert Reiter

IPC分类号： A01H5/00 ,  A01H1/00 ,  C07H21/04 ,  C12N15/82

CPC分类号： C12N9/0083 ,  C12N15/8247

摘要： The preparation and use of nucleic acid fragments encoding fatty acid desaturase enzymes are described. The invention permits alteration of plant lipid composition. Chimeric genes incorporating such nucleic acid fragments with suitable regulatory sequences may be used to create transgenic plants with altered levels of unsaturated fatty acids.

摘要翻译： 描述了编码脂肪酸去饱和酶的核酸片段的制备和使用。 本发明允许改变植物脂质组成。 使用合适的调控序列掺入此类核酸片段的嵌合基因可用于产生具有改变水平的不饱和脂肪酸的转基因植物。

公开(公告)号：US20080034457A1

公开(公告)日：2008-02-07

申请号：US11754923

申请日：2007-05-29

申请人： Lorin DeBonte ,  Willie Loh ,  Zhegong Fan

发明人： Lorin DeBonte ,  Willie Loh ,  Zhegong Fan

IPC分类号： A01H5/00

CPC分类号： A01H1/02 ,  A01H5/10 ,  A23D9/00 ,  C11B1/00 ,  C11C3/123

摘要： A canola line has been stabilized to produce seeds having an α-linolenic acid content of less than that of generic canola oil, more preferably lass-than or equal to about 7% α-linolenic acid relative to total fatty acid content of said seed and a total glucosinolate content of less than 18 μmol/g of defatted meal, more preferably less than or equal to about 15 μmol/g of defatted meal. This canola line has reduced sulfur content of less than or equal to 3.0 ppm, improved sensory characteristics and increased oxidative stability.

摘要翻译： 已经稳定了油菜籽油生产线，以产生α-亚麻酸含量小于普通卡诺拉油的种子，更优选相对于所述种子的总脂肪酸含量为大于或等于约7％的α-亚麻酸， 总硫代葡萄糖苷含量小于18mumol / g脱脂粉，更优选小于或等于约15mumol / g脱脂粉。 该油菜线具有降低的硫含量小于或等于3.0ppm，改善的感官特性和增加的氧化稳定性。

公开(公告)号：US20050262591A1

公开(公告)日：2005-11-24

申请号：US11108185

申请日：2005-04-18

申请人： Lorin Debonte ,  Basil Shorrosh

发明人： Lorin Debonte ,  Basil Shorrosh

IPC分类号： C07C53/126 ,  C07C57/02 ,  C07C57/03 ,  C12N9/02 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/04

CPC分类号： C12N15/8247 ,  C07C53/126 ,  C07C57/02 ,  C07C57/03 ,  C12N9/0083

摘要： Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

公开(公告)号：US20050114922A1

公开(公告)日：2005-05-26

申请号：US10976585

申请日：2004-10-26

申请人： Lorin DeBonte ,  Willie Loh ,  Zhegong Fan

发明人： Lorin DeBonte ,  Willie Loh ,  Zhegong Fan

IPC分类号： A01H1/02 ,  A01H5/10 ,  A23D9/00 ,  C11B1/00 ,  C11C3/12 ,  A01H1/00 ,  A01H5/00 ,  C12N15/82

CPC分类号： A01H1/02 ,  A01H5/10 ,  A23D9/00 ,  C11B1/00 ,  C11C3/123

摘要： A canola line has been stabilized to produce seeds having an α-linolenic acid content of less than that of generic canola oil, more preferably lass than or equal to about 7% α-linolenic acid relative to total fatty acid content of said seed and a total glucosinolate content of less than 18 μmol/g of defatted meal, more preferably less than or equal to about 15 μmol/g of defatted meal. This canola line has reduced sulfur content of less than or equal to 3.0 ppm, improved sensory characteristics and increased oxidative stability.

摘要翻译： 已经稳定了油菜籽油生产线以产生α-亚麻酸含量小于普通卡诺拉油的种子，更优选相对于所述种子的总脂肪酸含量为大于或等于约7％的α-亚麻酸，和 总硫代葡萄糖苷含量小于18mumol / g脱脂粉，更优选小于或等于约15mumol / g脱脂粉。 该油菜线具有降低的硫含量小于或等于3.0ppm，改善的感官特性和增加的氧化稳定性。