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    TRIMERIZING POLYPEPTIDES
    1.
    发明申请
    TRIMERIZING POLYPEPTIDES 失效
    调整多糖

    公开(公告)号：US20080220478A1

    公开(公告)日：2008-09-11

    申请号：US11530672

    申请日：2006-09-11

    申请人： Margaret Dow Moore ,  Brian A. Fox

    发明人： Margaret Dow Moore ,  Brian A. Fox

    IPC分类号： C12P21/02

    CPC分类号： C12N15/62 ,  A61K38/00 ,  C07K14/005 ,  C07K14/705 ,  C07K2319/03 ,  C07K2319/81 ,  C12N2740/10022 ,  C12N2740/13022

    摘要： The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译： 本发明涉及一种三聚蛋白的制备方法，其包括用编码包含ZymoZipper（ZZ）结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中，异源蛋白质是膜蛋白，融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域，并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中，ZZ结构域衍生自病毒包膜蛋白的跨膜（TM）亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子，表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。

    Materials and methods for preparing dimeric growth factors
    2.
    发明授权
    Materials and methods for preparing dimeric growth factors 失效
    制备二聚生长因子的材料和方法

    公开(公告)号：US07241593B2

    公开(公告)日：2007-07-10

    申请号：US10365095

    申请日：2003-02-11

    申请人： Brian A. Fox ,  Margaret Dow Moore ,  Kristine M. Swiderek ,  Carl W. Birks

    发明人： Brian A. Fox ,  Margaret Dow Moore ,  Kristine M. Swiderek ,  Carl W. Birks

    IPC分类号： C12P21/04 ,  C07K14/49

    CPC分类号： C07K14/49 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/735 ,  C12N2710/14043

    摘要： Proteins consisting of, from amino to carboxyl terminus, a first PDGF-D growth factor domain polypeptide, a linker polypeptide, and a second PDGF-D growth factor domain polypeptide, and materials and methods for making the proteins are disclosed. Each of the first and second PDGF-D growth factor domain polypeptides consists of a sequence of amino acid residues as shown in SEQ ID NO:2 or SEQ ID NO:4 from amino acid x to amino acid y, wherein x is an integer from 246 to 258, inclusive, and y is an integer from 365–370, inclusive. The linker polypeptide consists of from 11–40 amino acid residues. The proteins can be used to stimulate the production of bone and/or connective tissue in both humans and non-human animals.

    摘要翻译： 公开了由氨基至羧基末端组成第一PDGF-D生长因子结构域多肽，接头多肽和第二PDGF-D生长因子结构域多肽的蛋白质，以及用于制备蛋白质的材料和方法。 第一和第二PDGF-D生长因子结构域多肽中的每一个由从氨基酸x至氨基酸y的SEQ ID NO：2或SEQ ID NO：4所示的氨基酸残基序列组成，其中x是 246至258（含），y为365-370的整数，包括端值。 接头多肽由11-40个氨基酸残基组成。 蛋白质可用于刺激人和非人动物中骨和/或结缔组织的产生。

    Dimerized PDGF-D and materials and methods for producing it
    5.
    发明授权
    Dimerized PDGF-D and materials and methods for producing it 失效
    二聚PDGF-D及其制备方法

    公开(公告)号：US07122351B2

    公开(公告)日：2006-10-17

    申请号：US10274638

    申请日：2002-10-18

    申请人： Margaret Dow Moore ,  Brian A. Fox

    发明人： Margaret Dow Moore ,  Brian A. Fox

    IPC分类号： C12N5/10 ,  C12N15/16 ,  C12N15/63

    CPC分类号： C07K14/49 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2799/026

    摘要： Proteins consisting of two PDGF-D polypeptide chains, polynucleotides encoding the polypeptides, and materials and methods for making the proteins are disclosed. Each of the polypeptide chains consists of, from amino terminus to carboxyl terminus, the following operably linked segments: P1-P2-h-CH2-CH3; P1-P2-CH2-CH3; h-CH2-CH3-P2-P1; or CH2-CH3-P2-P1. Within these polypeptide chains, P1 is a first polypeptide segment as shown in SEQ ID NO:2 or SEQ ID NO:4 from amino acid x to amino acid y, wherein x is an integer from 246 to 258, inclusive, and y is an integer from 365–370, inclusive; P2 is a second polypeptide segment consisting of from 4 to 20 amino acid residues; h is an immunoglobulin hinge region or portion thereof; and CH2 and CH3 are CH2 and CH3 domains of an immunoglobulin heavy chain, respectively. Within the protein, the two polypeptide chains are joined by one or more disulfide bonds, each of the chains is optionally glycosylated, and the protein binds to and activates cell-surface PDGF receptors.

    摘要翻译： 公开了由两个PDGF-D多肽链组成的蛋白质，编码多肽的多核苷酸，以及用于制备蛋白质的材料和方法。 每个多肽链由氨基末端至羧基末端组成以下可操作连接的片段：P1-P2-h-C H-2-C 3 H 3; P1-P2-C H-2-C H 3; H-C H-2-C 3-P2-P1; 或C H 2 -C 3 -PO-P-2。 在这些多肽链中，P1是从氨基酸x至氨基酸y的SEQ ID NO：2或SEQ ID NO：4所示的第一个多肽区段，其中x是246至258的整数，包括端值，y是 整数为365-370，含 P2是由4至20个氨基酸残基组成的第二多肽区段; h是免疫球蛋白铰链区域或其部分; 和C H 2和C H 3是免疫球蛋白重链的C H 2 H 2和C 3 H 3结构域 ， 分别。 在蛋白质内，两条多肽链通过一个或多个二硫键连接，每个链任选被糖基化，并且蛋白质结合并激活细胞表面PDGF受体。

    Trimerizing polypeptides
    6.
    发明授权
    Trimerizing polypeptides 失效
    三聚多肽

    公开(公告)号：US07655439B2

    公开(公告)日：2010-02-02

    申请号：US11530672

    申请日：2006-09-11

    申请人： Margaret Dow Moore ,  Brian A. Fox

    发明人： Margaret Dow Moore ,  Brian A. Fox

    IPC分类号： C12P21/06

    CPC分类号： C12N15/62 ,  A61K38/00 ,  C07K14/005 ,  C07K14/705 ,  C07K2319/03 ,  C07K2319/81 ,  C12N2740/10022 ,  C12N2740/13022

    摘要： The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译： 本发明涉及一种三聚蛋白的制备方法，其包括用编码包含ZymoZipper（ZZ）结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中，异源蛋白质是膜蛋白，融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域，并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中，ZZ结构域衍生自病毒包膜蛋白的跨膜（TM）亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子，表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。

    Trimerizing polypeptides
    8.
    发明授权
    Trimerizing polypeptides 有权
    三聚多肽

    公开(公告)号：US08084230B2

    公开(公告)日：2011-12-27

    申请号：US12686896

    申请日：2010-01-13

    申请人： Margaret Dow Moore ,  Brian A. Fox

    发明人： Margaret Dow Moore ,  Brian A. Fox

    IPC分类号： C12P21/06

    CPC分类号： C12N15/62 ,  A61K38/00 ,  C07K14/005 ,  C07K14/705 ,  C07K2319/03 ,  C07K2319/81 ,  C12N2740/10022 ,  C12N2740/13022

    摘要： The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译： 本发明涉及一种三聚蛋白的制备方法，其包括用编码包含ZymoZipper（ZZ）结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中，异源蛋白质是膜蛋白，融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域，并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中，ZZ结构域衍生自病毒包膜蛋白的跨膜（TM）亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子，表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。

    TRIMERIZING POLYPEPTIDES
    10.
    发明申请
    TRIMERIZING POLYPEPTIDES 有权
    调整多糖

    公开(公告)号：US20100297701A1

    公开(公告)日：2010-11-25

    申请号：US12686896

    申请日：2010-01-13

    申请人： Margaret Dow Moore ,  Brian A. Fox

    发明人： Margaret Dow Moore ,  Brian A. Fox

    IPC分类号： C12P21/06

    CPC分类号： C12N15/62 ,  A61K38/00 ,  C07K14/005 ,  C07K14/705 ,  C07K2319/03 ,  C07K2319/81 ,  C12N2740/10022 ,  C12N2740/13022

    摘要： The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

    摘要翻译： 本发明涉及一种三聚蛋白的制备方法，其包括用编码包含ZymoZipper（ZZ）结构域和异源蛋白质的融合蛋白的表达载体转化或转染的宿主细胞。 在一个实施方案中，异源蛋白质是膜蛋白，融合蛋白中包含的异源蛋白质的部分是该蛋白质的细胞外结构域，并且所得的融合蛋白质是可溶的。 在本发明的另一个实施方案中，ZZ结构域衍生自病毒包膜蛋白的跨膜（TM）亚基或其它含有病毒基因组的七重复重复基因。 该方法可用于产生同型和异三聚体蛋白质。 本发明还包括本方法中使用的DNA分子，表达载体和宿主细胞以及通过本发明方法产生的融合蛋白。