公开(公告)号：US12036265B2

公开(公告)日：2024-07-16

申请号：US17362008

申请日：2021-06-29

申请人： Wayne State University

发明人： Hyeong-Reh C. Kim

IPC分类号： A61K38/18 ,  A61K35/28 ,  A61K47/64 ,  A61P19/08 ,  C07K14/49

CPC分类号： A61K38/1858 ,  A61K35/28 ,  A61K47/642 ,  A61P19/08 ,  C07K14/49

摘要： Methods of treating a subject in need thereof are provided which include: administering a recombinant platelet derived growth factor D (PDGF D) composition to a mesenchymal stem cell of the subject and/or a progenitor derived therefrom, in vivo, or ex vivo, producing a treated mesenchymal stem cell of the subject and/or a progenitor derived therefrom, thereby stimulating the mesenchymal stem cell (MSC) and/or a progenitor derived therefrom. Cells expressing a recombinant PDGF D composition of the present are administered to the subject for in vivo delivery of the recombinant PDGF D composition according to aspects of the present disclosure. Methods and compositions are provided including recombinant PDGF D hemidimer (HD) including a full-length PDGF D polypeptide and a C-terminal growth factor domain of PDGF D, which lacks a CUB domain, promoting regulation of bone marrow MSC differentiation into osteogenic lineage cells.

公开(公告)号：US20240189423A1

公开(公告)日：2024-06-13

申请号：US18553447

申请日：2022-03-30

申请人： The Regents of the University of California

发明人： James A. WELLS ,  Katarina PANCE ,  Josef A. GRAMESPACHER ,  Kaan Kumru

IPC分类号： A61K39/00 ,  A61K47/68 ,  A61P35/00 ,  C07K14/475 ,  C07K14/48 ,  C07K14/49 ,  C07K14/495 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/52 ,  C07K14/525 ,  C07K14/53 ,  C07K14/54 ,  C07K14/55 ,  C07K14/56 ,  C07K14/565 ,  C07K14/57 ,  C07K14/575 ,  C07K14/61 ,  C07K14/65 ,  C07K16/28 ,  C12N5/0781

CPC分类号： A61K39/4644 ,  A61K39/4612 ,  A61K39/4633 ,  A61K47/6851 ,  A61P35/00 ,  C07K14/475 ,  C07K14/48 ,  C07K14/49 ,  C07K14/495 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/521 ,  C07K14/525 ,  C07K14/53 ,  C07K14/5403 ,  C07K14/5406 ,  C07K14/5409 ,  C07K14/5412 ,  C07K14/5418 ,  C07K14/5425 ,  C07K14/5431 ,  C07K14/5434 ,  C07K14/5437 ,  C07K14/55 ,  C07K14/56 ,  C07K14/565 ,  C07K14/57 ,  C07K14/575 ,  C07K14/61 ,  C07K14/65 ,  C07K16/2803 ,  C07K16/2887 ,  C12N5/0635 ,  C07K2317/31 ,  C07K2319/30 ,  C12N2510/00

摘要： The present disclosure relates to targeted degradation platform technology. For example, the present disclosure relates to bispecific binding agents for degrading endogenous proteins, whether membrane-associated or soluble, using the lysosome pathway. The disclosure also provides methods useful for producing such agents, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for the treatment of various disorders.

公开(公告)号：US20230340024A1

公开(公告)日：2023-10-26

申请号：US18331851

申请日：2023-06-08

申请人： Trustees of Tufts College

发明人： Rajendra Kumar-Singh

IPC分类号： C07K7/08 ,  A61P27/02 ,  A61K47/42 ,  C07K7/06 ,  C07K14/47 ,  C07K14/49 ,  C12N15/86

CPC分类号： C07K7/08 ,  A61P27/02 ,  A61K47/42 ,  C07K7/06 ,  C07K14/47 ,  C07K14/49 ,  C12N15/86 ,  A61K38/00

摘要： Cell penetrating peptides that target many cells types including cells of the retina and cornea with high efficiency are provided herein. These peptides can be used to deliver cargo molecules across a plasma membrane, without the need for chemical conjugation. Compositions and viral vectors comprising these cell-penetrating peptides are also provided. Methods of using the peptides, compositions and viruses to deliver various agents to target cells and tissues are also provided.

公开(公告)号：US20230250132A1

公开(公告)日：2023-08-10

申请号：US17990438

申请日：2022-11-18

申请人： UBIPROTEIN, CORP.

发明人： Kyunggon Kim ,  Kwang-Hyun Baek ,  Sung-Ryul Bae ,  Myung-Sun Kim ,  Hyeonmi Kim ,  Yeeun Yoo ,  Lan Li ,  Jung-Hyun Park ,  Jin-Ok Kim

IPC分类号： C07K1/107 ,  C07K14/49 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/535 ,  C07K14/56 ,  C07K14/565 ,  C07K14/575 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K16/00

CPC分类号： C07K1/1075 ,  C07K14/49 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/535 ,  C07K14/56 ,  C07K14/565 ,  C07K14/575 ,  C07K14/5759 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K16/00 ,  A61K38/00

摘要： The present invention relates to a method for prolonging half-life of a protein or a (poly)peptide by replacing one or more amino acid residues of the protein. Further, the present invention is about the protein having a prolonged half-life prepared by the method above.

公开(公告)号：US20230242576A1

公开(公告)日：2023-08-03

申请号：US17990507

申请日：2022-11-18

申请人： UBIPROTEIN, CORP.

发明人： Kyunggon Kim ,  Kwang-Hyun Baek ,  Sung-Ryul Bae ,  Myung-Sun Kim ,  Hyeonmi Kim ,  Yeeun Yoo ,  Lan Li ,  Jung-Hyun Park ,  Jin-Ok Kim

IPC分类号： C07K1/107 ,  C07K14/49 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/535 ,  C07K14/56 ,  C07K14/565 ,  C07K14/575 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K16/00

CPC分类号： C07K1/1075 ,  C07K14/49 ,  C07K14/50 ,  C07K14/505 ,  C07K14/51 ,  C07K14/535 ,  C07K14/56 ,  C07K14/565 ,  C07K14/575 ,  C07K14/5759 ,  C07K14/605 ,  C07K14/61 ,  C07K14/62 ,  C07K16/00 ,  A61K38/00

摘要： The present invention relates to a method for prolonging half-life of a protein or a (poly)peptide by replacing one or more amino acid residues of the protein. Further, the present invention is about the protein having a prolonged half-life prepared by the method above.

公开(公告)号：US20180271987A1

公开(公告)日：2018-09-27

申请号：US15736407

申请日：2016-06-23

申请人： The University of Nottingham

发明人： James Dixon

IPC分类号： A61K47/64 ,  A61K33/26 ,  A61K41/00

CPC分类号： A61K47/64 ,  A61K33/26 ,  A61K41/0052 ,  A61K47/62 ,  C07K14/485 ,  C07K14/49 ,  C07K14/50 ,  C07K17/14 ,  C07K2319/10 ,  C07K2319/20 ,  C07K2319/33 ,  C12N15/62

摘要： The invention relates to apH mediated cell delivery vehicle comprising: a cargo or a cargo-binding molecule for binding to a cargo; a protein transduction domain; and a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell, wherein the GAG binding element is a peptide which is modified to comprise one or more histidine residues which are capable of being protonated in an acidic environment. The invention further relates to the use of such a delivery vehicle including methods of treatment, such as treatment for cancer.

公开(公告)号：US09789162B2

公开(公告)日：2017-10-17

申请号：US14932826

申请日：2015-11-04

申请人： The United States of America, as represented by the Department of Veterans Affairs ,  Loma Linda University

发明人： David Baylink ,  Kin-Hing William Lau ,  Wanqiu Chen ,  Xiao-Bing Zhang

IPC分类号： C12N5/0789 ,  C12N5/10 ,  C12N15/63 ,  A61K38/18 ,  C07K14/49 ,  C12N15/86 ,  C12N7/00 ,  A61K35/28 ,  A61K47/48 ,  C12N5/0775 ,  A61K48/00

CPC分类号： A61K38/1858 ,  A61K35/28 ,  A61K47/548 ,  A61K47/62 ,  A61K47/64 ,  A61K48/00 ,  A61K48/005 ,  C07K14/49 ,  C12N5/0663 ,  C12N7/00 ,  C12N15/86 ,  C12N2501/135 ,  C12N2510/00 ,  C12N2740/15043 ,  C12N2740/15071 ,  C12N2800/00

摘要： Methods are disclosed herein for increasing bone mass and strength or bone fracture healing in a subject. The methods include administering to the subject a therapeutically effective amount of multipotent stem cells, wherein each multipotent stem cell is transformed with a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a nucleic acid encoding platelet derived growth factor (PDGF) B, and wherein the multipotent stem cells express a sufficient amount of PDGFB to increase bone mass and strength or bone fracture healing. A lentiviral vector also is disclosed that includes a phosphoglycerate kinase-1 (PGK) promoter operably linked to a nucleic acid encoding PDGFB.

公开(公告)号：US20160324989A1

公开(公告)日：2016-11-10

申请号：US15110211

申请日：2015-01-13

申请人： WAKE FOREST UNIVERSITY HEALTH SCIENCES

发明人： King C. Li ,  Akiva Mintz ,  Xiaobing Xiong ,  Youngkyoo Jung ,  Yao Sun

IPC分类号： A61K48/00 ,  A61K45/06 ,  A61K38/19 ,  A61K38/18 ,  C07K14/525 ,  A61M37/00 ,  C07K14/49 ,  A61K35/30 ,  C07K14/52 ,  A61K9/00 ,  A61K41/00 ,  C12N15/86 ,  A61K35/28 ,  C07K14/495

CPC分类号： A61K48/0083 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/30 ,  A61K35/545 ,  A61K38/1841 ,  A61K38/1866 ,  A61K38/19 ,  A61K38/191 ,  A61K41/0047 ,  A61K45/06 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0066 ,  A61M37/0092 ,  A61P35/00 ,  C07K14/49 ,  C07K14/495 ,  C07K14/52 ,  C07K14/525 ,  C12N15/86 ,  C12N2830/002

摘要： A method of increasing blood-brain barrier permeability of selected brain tissue in a subject in need thereof is carried out by: (a) parenterally administering to the subject stem cells that migrate to the brain tissue, the stem cells containing a recombinant nucleic acid, the recombinant nucleic acid comprising a nucleic acid encoding a barrier-opening protein or peptide operably associated with a heat-inducible promoter; and then (b) selectively heating the selected brain tissue sufficient to induce the expression of the barrier-opening protein or peptide in an amount effective to increase the permeability of the blood-brain barrier in the selected brain tissue. Nucleic acids, vectors, stem cells and compositions useful for carrying out such methods are also described.

摘要翻译： 通过以下方法进行选择性脑组织中选择的脑组织的血脑屏障通透性的方法：（a）胃肠外给予迁移至脑组织的干细胞，含有重组核酸的干细胞， 所述重组核酸包含编码与热诱导型启动子可操作地相关联的阻断开启蛋白或肽的核酸; 然后（b）选择性地加热所选择的脑组织足以诱导阻断开启蛋白或肽的表达，其量可以有效增加所选择的脑组织中血脑屏障的通透性。 还描述了用于实施这些方法的核酸，载体，干细胞和组合物。

公开(公告)号：US20160297868A1

公开(公告)日：2016-10-13

申请号：US14917460

申请日：2014-10-01

申请人： UNIVERSITY OF MISSISSIPPI MEDICAL CENTER

发明人： Gene L. Bidwell, III ,  Eric M. George

IPC分类号： C07K14/78 ,  C07K7/08 ,  A61K38/17 ,  C07K14/47 ,  A61K38/18 ,  C07K14/49

CPC分类号： C07K14/78 ,  A61K38/005 ,  A61K38/1866 ,  A61K47/6435 ,  C07K7/08 ,  C07K14/4703 ,  C07K14/49 ,  C07K2319/00 ,  C07K2319/33

摘要： A composition including an elastin-like polypeptide (ELP) coupled to a therapeutic agent is provided. The ELP comprises at least about 5 repeats of the amino acid sequence VPGXG. Further provided is a method of using the composition for therapeutic agent delivery during pregnancy to reduce the amount of the therapeutic agent crossing a placenta in a pregnant subject. The method includes administering to the pregnant subject an effective amount of the composition comprising the ELP coupled to the therapeutic agent.

摘要翻译： 提供了包含与治疗剂偶联的弹性蛋白样多肽（ELP）的组合物。 ELP包含至少约5个重复的氨基酸序列VPGXG。 进一步提供的是使用该组合物用于怀孕期间治疗剂递送的方法，以减少在怀孕受试者中穿过胎盘的治疗剂的量。 该方法包括向孕妇施用有效量的包含与治疗剂偶联的ELP的组合物。

公开(公告)号：US20160120944A1

公开(公告)日：2016-05-05

申请号：US14932826

申请日：2015-11-04

申请人： The United States Government as represented by the Department of Veterans Affairs ,  Loma Linda University

发明人： David Baylink ,  Kin-Hing William Lau ,  Wanqiu Chen ,  Xiao-Bing Zhang

IPC分类号： A61K38/18 ,  A61K35/28 ,  C12N7/00 ,  C07K14/49 ,  C12N15/86

CPC分类号： A61K38/1858 ,  A61K35/28 ,  A61K47/548 ,  A61K47/62 ,  A61K47/64 ,  A61K48/00 ,  A61K48/005 ,  C07K14/49 ,  C12N5/0663 ,  C12N7/00 ,  C12N15/86 ,  C12N2501/135 ,  C12N2510/00 ,  C12N2740/15043 ,  C12N2740/15071 ,  C12N2800/00

摘要： Methods are disclosed herein for increasing bone mass and strength or bone fracture healing in a subject. The methods include administering to the subject a therapeutically effective amount of multipotent stem cells, wherein each multipotent stem cell is transformed with a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a nucleic acid encoding platelet derived growth factor (PDGF) B, and wherein the multipotent stem cells express a sufficient amount of PDGFB to increase bone mass and strength or bone fracture healing. A lentiviral vector also is disclosed that includes a phosphoglycerate kinase-1 (PGK) promoter operably linked to a nucleic acid encoding PDGFB.

摘要翻译： 本文公开了用于增加受试者的骨质量和强度或骨折愈合的方法。 所述方法包括向受试者施用治疗有效量的多能干细胞，其中每个多能干细胞用包含与编码血小板衍生生长因子（PDGF）B的核酸可操作连接的异源启动子的重组核酸分子转化，以及 其中所述多能干细胞表达足够量的PDGFB以增加骨量和强度或骨折愈合。 还公开了包含与编码PDGFB的核酸可操作地连接的磷酸甘油酸激酶-1（PGK）启动子的慢病毒载体。