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公开(公告)号：US10066257B2
公开(公告)日：2018-09-04
申请号：US13474596
申请日：2012-05-17
IPC分类号： C12Q1/68 , C07H21/04 , C07H21/00 , C12Q1/6813 , C12Q1/6809 , C12Q1/6834 , C12Q1/6855 , C12Q1/6862
摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.
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公开(公告)号：US09745616B2
公开(公告)日：2017-08-29
申请号：US14118524
申请日：2012-05-17
CPC分类号： C12Q1/6813 , C12Q1/6809 , C12Q1/6834 , C12Q1/6855 , C12Q1/6862 , C12Q2523/109 , C12Q2525/155 , C12Q2525/161 , C12Q2525/197 , C12Q2525/204 , C12Q2533/107 , C12Q2537/143 , C12Q2563/131 , C12Q2563/179 , C12Q2565/125 , C12Q2525/15
摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.
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公开(公告)号：US20130005594A1
公开(公告)日：2013-01-03
申请号：US13474596
申请日：2012-05-17
CPC分类号： C12Q1/6813 , C12Q1/6809 , C12Q1/6834 , C12Q1/6855 , C12Q1/6862 , C12Q2523/109 , C12Q2525/155 , C12Q2525/161 , C12Q2525/197 , C12Q2525/204 , C12Q2533/107 , C12Q2537/143 , C12Q2563/131 , C12Q2563/179 , C12Q2565/125 , C12Q2525/15
摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.
摘要翻译： 本发明提供了用于检测样品中存在的一种或多种核酸靶的组合物，装置和方法。 本发明的方法包括利用两个或更多个连接探针，其将靶核酸彼此紧密结合并且具有互补的反应性连接部分。 当这样的探针以正确的方向结合靶时，它们能够经历自发化学连接反应，产生直接检测到的连接产物或被扩增以产生随后检测到的扩增子的连接产物。 本发明还提供了稳定样品RNA使得降解不显着影响分析结果的方法。
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