公开(公告)号：US20060160153A1

公开(公告)日：2006-07-20

申请号：US10537500

申请日：2003-12-02

申请人： Jean-Pierre Abastado ,  Nadege Bercovici ,  Marc Ernstoff ,  Alice Givan ,  Alessandra Nardin ,  Margarita Magguilli born Salcedo ,  Paul Wallace

发明人： Jean-Pierre Abastado ,  Nadege Bercovici ,  Marc Ernstoff ,  Alice Givan ,  Alessandra Nardin ,  Margarita Magguilli born Salcedo ,  Paul Wallace

IPC分类号： G01N33/567

CPC分类号： G01N33/5094 ,  G01N33/505 ,  G01N33/56972

摘要： A method to characterize a T-cell response of a final population of T-lymphocytes resulting from the co-incubation of an initial population of T lymphocytes with a composition of antigen-presenting cells (APCs), said method comprising the steps of a) simultaneous measuring on a single cell basis at least two parameters: (i) proliferation of T lymphocytes and (ii) presence of a T cell antigen receptor on the surface of T lymphocytes and/or presence of at least one biological molecule produced by T lymphocytes, and the attribution of a positive or a negative value to each of these parameters, and b) classification of the final T-lymphocytes population into 2n different subsets of T lymphocytes, n being the number of parameters, each subset being characterized by a positive or a negative value respectively to each parameter and the determination of the proportion of T lymphocytes present in each subset with respect to the number of T lymphocytes in the final population, with said proportion being characteristic of the T-cell response. Use of the method for batch release assay and potency assay.

摘要翻译： 表征由初始T淋巴细胞群与抗原呈递细胞（APC）的组合共孵育的最终T淋巴细胞群的T细胞应答的方法，所述方法包括以下步骤：a） 在单细胞基础上同时测量至少两个参数：（i）T淋巴细胞的增殖和（ii）T淋巴细胞表面上存在T细胞抗原受体和/或由T淋巴细胞产生的至少一种生物分子的存在 ，以及对这些参数中的每一个的正值或负值的归属，以及b）将最终T淋巴细胞群分类为T淋巴细胞的2个不同子集，n是参数的数目 每个子集分别以每个参数的正值或负值表示，并确定每个子集中存在的T淋巴细胞相对于最终群体中T淋巴细胞数目的比例，其中sa id比例是T细胞应答的特征。 使用批次释放测定法和效力测定法。