公开(公告)号：US08639449B2

公开(公告)日：2014-01-28

申请号：US13097430

申请日：2011-04-29

申请人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

IPC分类号： G01N33/48 ,  G01N33/50

CPC分类号： H01L33/0062 ,  B82Y15/00 ,  C09K11/025 ,  C09K11/565 ,  C09K11/883 ,  C09K11/89 ,  C09K11/892 ,  C12Q1/6816 ,  C12Q2563/107 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  H01L33/0083 ,  Y10T436/141111 ,  Y10T436/142222

摘要： A semiconductor nanocrystal compound and probe are described. The compound is capable of linking to one or more affinity molecules. The compound comprises (1) one or more semiconductor nanocrystals capable of, in response to exposure to a first energy, providing a second energy, and (2) one or more linking agents, having a first portion linked to the one or more semiconductor nanocrystals and a second portion capable of linking to one or more affinity molecules. One or more semiconductor nanocrystal compounds are linked to one or more affinity molecules to form a semiconductor nanocrystal probe capable of bonding with one or more detectable substances in a material being analyzed, and capable of, in response to exposure to a first energy, providing a second energy. Also described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and treating materials with the probe.

摘要翻译： 描述了半导体纳米晶体化合物和探针。 该化合物能够连接至一个或多个亲和分子。 化合物包括（1）能够响应于暴露于第一能量提供第二能量的一种或多种半导体纳米晶体，和（2）一种或多种连接剂，其具有连接到所述一种或多种半导体纳米晶体的第一部分 和能够连接到一个或多个亲和分子的第二部分。 一个或多个半导体纳米晶体化合物与一个或多个亲和分子连接以形成半导体纳米晶体探针，该半导体纳米晶体探针能够与正在分析的材料中的一种或多种可检测物质结合，并且能够响应于暴露于第一能量而提供 第二节能量。 还描述了分别制造半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并用探针处理材料。

公开(公告)号：US08158194B2

公开(公告)日：2012-04-17

申请号：US12624283

申请日：2009-11-23

申请人： Edward Adams ,  Marcel Bruchez

发明人： Edward Adams ,  Marcel Bruchez

IPC分类号： B05D7/00

CPC分类号： B82Y15/00 ,  C09K11/02 ,  C09K11/883 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2998 ,  Y10T428/31928

摘要： Water-dispersible nanoparticles are prepared by applying a coating of a amphipathic dispersant to the surface of a hydrophobic nanoparticle comprised of a semiconductive or metallic material. The amphipathic dispersant is typically polymeric. Preferred polymeric dispersants are comprised of (1) a hydrophobic backbone with hydrophilic branches, (2) a hydrophilic backbone with hydrophobic branches, or (3) a backbone that may be either hydrophobic or hydrophilic, and substituted with both hydrophilic and hydrophobic branches. Monodisperse populations of water-dispersible nanoparticles are also provided, as are conjugates of the water-dispersible nanoparticles with affinity molecules such as peptides, oligonucleotides, and the like.

摘要翻译： 通过将两性分散剂的涂层施加到由半导体或金属材料构成的疏水性纳米颗粒的表面来制备水分散性纳米颗粒。 两亲性分散剂通常是聚合物。 优选的聚合物分散剂包括（1）具有亲水分支的疏水主链，（2）具有疏水分支的亲水性骨架，或（3）可以是疏水性或亲水性的主链，并且被亲水和疏水分支两者取代。 还提供了水分散性纳米颗粒的单分散群体，以及水分散性纳米颗粒与亲和分子如肽，寡核苷酸等的缀合物​​。

公开(公告)号：US08071361B2

公开(公告)日：2011-12-06

申请号：US12714284

申请日：2010-02-26

申请人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

IPC分类号： C12M1/00 ,  C12Q1/68

CPC分类号： H01L33/0062 ,  B82Y15/00 ,  C09K11/025 ,  C09K11/565 ,  C09K11/883 ,  C09K11/89 ,  C09K11/892 ,  C12Q1/6816 ,  C12Q2563/107 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  H01L33/0083 ,  Y10T436/141111 ,  Y10T436/142222

摘要： A semiconductor nanocrystal compound and probe are described. The compound is capable of linking to one or more affinity molecules. The compound comprises (1) one or more semiconductor nanocrystals capable of, in response to exposure to a first energy, providing a second energy, and (2) one or more linking agents, having a first portion linked to the one or more semiconductor nanocrystals and a second portion capable of linking to one or more affinity molecules. One or more semiconductor nanocrystal compounds are linked to one or more affinity molecules to form a semiconductor nanocrystal probe capable of bonding with one or more detectable substances in a material being analyzed, and capable of, in response to exposure to a first energy, providing a second energy. Also described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and treating materials with the probe.

公开(公告)号：US20060177945A1

公开(公告)日：2006-08-10

申请号：US11370656

申请日：2006-03-07

申请人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

IPC分类号： G01N33/551

CPC分类号： C12Q1/6816 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T436/24 ,  C12Q2563/107

摘要： A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) an affinity molecule linked to the semiconductor nanocrystal. The semiconductor nanocrystal is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Exposure of the semiconductor nanocrystal to excitation energy will excite the semiconductor nanocrystal causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

摘要翻译： 描述能够连接到亲和分子的半导体纳米晶体化合物。 化合物包括（1）当被电磁辐射源或粒子束激发时能够发射电磁辐射和/或吸收能量和/或散射或衍射电磁辐射的半导体纳米晶体; 和（2）与半导体纳米晶体连接的亲和分子。 半导体纳米晶体与亲和分子连接以形成能够与可检测物质结合的半导体纳米晶体探针。 将半导体纳米晶体暴露于激发能将激发导致电磁辐射发射的半导体纳米晶体。 进一步描述的是分别制造发光半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并使用探针来确定材料中可检测物质的存在。

公开(公告)号：US06927069B2

公开(公告)日：2005-08-09

申请号：US10155759

申请日：2002-05-24

申请人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

IPC分类号： C12Q1/68 ,  G01N33/533 ,  G01N33/542 ,  G01N33/543 ,  G01N33/58 ,  G01Q70/08 ,  G01Q70/18

CPC分类号： C12Q1/6816 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T436/24 ,  C12Q2563/107

摘要： A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

摘要翻译： 描述能够连接到亲和分子的半导体纳米晶体化合物。 化合物包括（1）当被电磁辐射源或粒子束激发时能够发射电磁辐射和/或吸收能量和/或散射或衍射电磁辐射的半导体纳米晶体; 和（2）至少一种连接剂，其具有连接到半导体纳米晶体的第一部分和能够连接到亲和分子的第二部分。 该化合物与亲和分子连接以形成能够与可检测物质结合的半导体纳米晶体探针。 随后暴露于激发能量将激发探针中的半导体纳米晶体，导致电磁辐射的发射。 进一步描述的是分别制造发光半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并使用探针来确定材料中可检测物质的存在。

公开(公告)号：US07314764B2

公开(公告)日：2008-01-01

申请号：US11370656

申请日：2006-03-07

申请人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

IPC分类号： G01N33/552

CPC分类号： C12Q1/6816 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T436/24 ,  C12Q2563/107

摘要： A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) an affinity molecule linked to the semiconductor nanocrystal. The semiconductor nanocrystal is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Exposure of the semiconductor nanocrystal to excitation energy will excite the semiconductor nanocrystal causing the emission of electromagnetic radiation. Further described are processes for respectively: making the luminescent semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

摘要翻译： 描述能够连接到亲和分子的半导体纳米晶体化合物。 化合物包括（1）当被电磁辐射源或粒子束激发时能够发射电磁辐射和/或吸收能量和/或散射或衍射电磁辐射的半导体纳米晶体; 和（2）与半导体纳米晶体连接的亲和分子。 半导体纳米晶体与亲和分子连接以形成能够与可检测物质结合的半导体纳米晶体探针。 将半导体纳米晶体暴露于激发能将激发导致电磁辐射发射的半导体纳米晶体。 进一步描述的是分别制造发光半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并使用探针来确定材料中可检测物质的存在。

公开(公告)号：US20070111350A1

公开(公告)日：2007-05-17

申请号：US11566998

申请日：2006-12-05

申请人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

IPC分类号： H01L21/00 ,  H01L33/00 ,  H01G9/20

CPC分类号： H01L33/0062 ,  B82Y15/00 ,  C09K11/025 ,  C09K11/565 ,  C09K11/883 ,  C09K11/89 ,  C09K11/892 ,  C12Q1/6816 ,  C12Q2563/107 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  H01L33/0083 ,  Y10T436/141111 ,  Y10T436/142222

摘要： A semiconductor nanocrystal compound and probe are described. The compound is capable of linking to one or more affinity molecules. The compound comprises (1) one or more semiconductor nanocrystals capable of, in response to exposure to a first energy, providing a second energy, and (2) one or more linking agents, having a first portion linked to the one or more semiconductor nanocrystals and a second portion capable of linking to one or more affinity molecules. One or more semiconductor nanocrystal compounds are linked to one or more affinity molecules to form a semiconductor nanocrystal probe capable of bonding with one or more detectable substances in a material being analyzed, and capable of, in response to exposure to a first energy, providing a second energy. Also described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and treating materials with the probe.

摘要翻译： 描述了半导体纳米晶体化合物和探针。 该化合物能够连接至一个或多个亲和分子。 化合物包括（1）能够响应于暴露于第一能量提供第二能量的一种或多种半导体纳米晶体，和（2）一种或多种连接剂，其具有连接到所述一种或多种半导体纳米晶体的第一部分 和能够连接到一个或多个亲和分子的第二部分。 一个或多个半导体纳米晶体化合物与一个或多个亲和分子连接以形成半导体纳米晶体探针，该半导体纳米晶体探针能够与正在分析的材料中的一种或多种可检测物质结合，并且能够响应于暴露于第一能量而提供 第二节能量。 还描述了分别制造半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并用探针处理材料。

公开(公告)号：US06699723B1

公开(公告)日：2004-03-02

申请号：US09865130

申请日：2001-05-24

申请人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

IPC分类号： G01N33543

CPC分类号： B82Y15/00 ,  G01N33/54373 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T436/24

摘要： A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affinity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in the probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

摘要翻译： 描述能够连接到亲和分子的半导体纳米晶体化合物。 化合物包括（1）当被电磁辐射源或粒子束激发时能够发射电磁辐射和/或吸收能量和/或散射或衍射电磁辐射的半导体纳米晶体; 和（2）至少一种连接剂，其具有连接到半导体纳米晶体的第一部分和能够连接到亲和分子的第二部分。 该化合物与亲和分子连接以形成能够与可检测物质结合的半导体纳米晶体探针。 随后暴露于激发能量将激发探针中的半导体纳米晶体，导致电磁辐射的发射。 进一步描述了分别制造半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并使用探针来确定材料中可检测物质的存在。

公开(公告)号：US06423551B1

公开(公告)日：2002-07-23

申请号：US09349833

申请日：1999-07-08

申请人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez, Jr. ,  Paul Alivisatos

IPC分类号： G01N33543

CPC分类号： C12Q1/6816 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  Y10T428/2982 ,  Y10T428/2989 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2996 ,  Y10T436/24 ,  C12Q2563/107

摘要： A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation—when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in he probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

摘要翻译： 描述能够连接到亲和分子的半导体纳米晶体化合物。 化合物包括（1）当被电磁辐射源或粒子束激发时能够发射电磁辐射和/或吸收能量和/或散射或衍射电磁辐射的半导体纳米晶体; 和（2）具有连接到半导体纳米晶体的第一部分和能够连接到亲和分子的第二部分的至少一种连接剂。 该化合物与亲和分子连接以形成能够与可检测物质结合的半导体纳米晶体探针。 随后暴露于激发能将激发探针中的半导体纳米晶体，导致电磁辐射的发射。 进一步描述了分别制造半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并使用探针来确定材料中可检测物质的存在。

公开(公告)号：US08288153B2

公开(公告)日：2012-10-16

申请号：US12408462

申请日：2009-03-20

申请人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

发明人： Shimon Weiss ,  Marcel Bruchez ,  Paul Alivisatos

IPC分类号： C12M1/00 ,  C12Q1/68

CPC分类号： H01L33/0062 ,  B82Y15/00 ,  C09K11/025 ,  C09K11/565 ,  C09K11/883 ,  C09K11/89 ,  C09K11/892 ,  C12Q1/6816 ,  C12Q2563/107 ,  G01N33/533 ,  G01N33/542 ,  G01N33/54373 ,  G01N33/588 ,  H01L33/0083 ,  Y10T436/141111 ,  Y10T436/142222

摘要： A semiconductor nanocrystal compound and probe are described. The compound is capable of linking to one or more affinity molecules. The compound comprises (1) one or more semiconductor nanocrystals capable of, in response to exposure to a first energy, providing a second energy, and (2) one or more linking agents, having a first portion linked to the one or more semiconductor nanocrystals and a second portion capable of linking to one or more affinity molecules. One or more semiconductor nanocrystal compounds are linked to one or more affinity molecules to form a semiconductor nanocrystal probe capable of bonding with one or more detectable substances in a material being analyzed, and capable of, in response to exposure to a first energy, providing a second energy. Also described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and treating materials with the probe.

摘要翻译： 描述了半导体纳米晶体化合物和探针。 该化合物能够连接至一个或多个亲和分子。 化合物包括（1）能够响应于暴露于第一能量提供第二能量的一种或多种半导体纳米晶体，和（2）一种或多种连接剂，其具有连接到所述一种或多种半导体纳米晶体的第一部分 和能够连接到一个或多个亲和分子的第二部分。 一个或多个半导体纳米晶体化合物与一个或多个亲和分子连接以形成半导体纳米晶体探针，该半导体纳米晶体探针能够与正在分析的材料中的一种或多种可检测物质结合，并且能够响应于暴露于第一能量而提供 第二节能量。 还描述了分别制造半导体纳米晶体化合物的方法; 制造半导体纳米晶体探针; 并用探针处理材料。