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公开(公告)号:US11493507B2
公开(公告)日:2022-11-08
申请号:US13496023
申请日:2010-09-07
申请人: Wendy Uyen Dittmer , Toon Hendrik Evers , Marco Hendrikus Hefti , David Walterus Cornelis Dekkers , Michael Franciscus Wilhelmus Cornelis Martens
发明人: Wendy Uyen Dittmer , Toon Hendrik Evers , Marco Hendrikus Hefti , David Walterus Cornelis Dekkers , Michael Franciscus Wilhelmus Cornelis Martens
IPC分类号: G01N33/543
摘要: An immunoassay for the detection of an analyte in a sample includes a plurality of moieties capable of binding to the analyte. Capture moieties, which are not specific for the same epitope, are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker. The detectable marker is a large particle marker having a particle size of ≥50 nm and ≤5000 nm.
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公开(公告)号:US20120258553A1
公开(公告)日:2012-10-11
申请号:US13518428
申请日:2010-12-16
申请人: Wendy Uyen Dittmer , Toon Hendrik Evers , David Walterus Cornelis Dekkers , Marco Hendrikus Hefti , Joost Lambert Max Vissers , Michael Franciscus Wilhelmus Cornelis Martens
发明人: Wendy Uyen Dittmer , Toon Hendrik Evers , David Walterus Cornelis Dekkers , Marco Hendrikus Hefti , Joost Lambert Max Vissers , Michael Franciscus Wilhelmus Cornelis Martens
IPC分类号: G01N27/72
CPC分类号: G01N33/54333 , G01N21/552 , G01N27/745 , G01N33/54393 , G01N2446/00
摘要: An apparatus for measuring a target molecule in a sample is disclosed. The apparatus comprises a moiety comprising a magnetic label (1) greater than 100 nm, a binding surface (12) for specifically binding the moiety, the amount of said moiety binding to said surface being indicative of the amount of the target molecule in said sample, detection means (31 31′) for detecting the amount of said moiety bound to said surface, and a salt (51) for reducing aggregation of the magnetic labels of respective moieties in said sample. The apparatus preferably also comprises a magnetic field generator (41) for attracting the magnetic labels to the binding surface. A method for measuring a target molecule in a sample and a disposable cartridge for use with the apparatus are also disclosed.
摘要翻译: 公开了一种用于测量样品中靶分子的装置。 该装置包括包含大于100nm的磁性标签(1)的部分,用于特异性结合该部分的结合表面(12),所述部分结合所述表面的量指示所述样品中靶分子的量 ,用于检测与所述表面结合的所述部分的量的检测装置(31 31')和用于减少所述样品中各部分的磁性标记物的聚集的盐(51)。 该装置优选地还包括用于将磁性标签吸引到装订表面的磁场发生器(41)。 还公开了一种用于测量样品中的靶分子的方法和用于该设备的一次性盒。
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公开(公告)号:US20120171781A1
公开(公告)日:2012-07-05
申请号:US13496023
申请日:2010-09-07
申请人: Wendy Uyen Dittmer , Toon Hendrik Evers , Marco Hendrikus Hefti , David Walterus Cornelis Dekkers , Michael Franciscus Wilhelmus Cornelis Martens
发明人: Wendy Uyen Dittmer , Toon Hendrik Evers , Marco Hendrikus Hefti , David Walterus Cornelis Dekkers , Michael Franciscus Wilhelmus Cornelis Martens
IPC分类号: G01N33/566 , G01N21/75 , G01N33/577
CPC分类号: G01N33/54346 , G01N33/54333 , G01N2333/58
摘要: The present invention is related to an immunoassay for the detection of an analyte in a sample, said assay comprising a plurality of moieties capable of binding to said analyte, wherein capture moieties which are not specific for the same epitope are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker, and wherein the detectable marker to which the epitope-specific detection moiety is bound is a large particle marker having a particle size of ≧50 nm and ≦5000 nm.
摘要翻译: 本发明涉及用于检测样品中分析物的免疫测定法,所述测定法包括能够结合所述分析物的多个部分,其中对相同表位不特异的捕获部分结合到固体底物上, 并且至少一个表位特异性检测部分结合到可检测标记上,并且其中表位特异性检测部分结合的可检测标记是具有≥50nm和< NE; 5000nm的粒度的大颗粒标记物。
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