公开(公告)号：US11493507B2

公开(公告)日：2022-11-08

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/543

摘要： An immunoassay for the detection of an analyte in a sample includes a plurality of moieties capable of binding to the analyte. Capture moieties, which are not specific for the same epitope, are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker. The detectable marker is a large particle marker having a particle size of ≥50 nm and ≤5000 nm.

公开(公告)号：US20120258553A1

公开(公告)日：2012-10-11

申请号：US13518428

申请日：2010-12-16

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  David Walterus Cornelis Dekkers ,  Marco Hendrikus Hefti ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N27/72

CPC分类号： G01N33/54333 ,  G01N21/552 ,  G01N27/745 ,  G01N33/54393 ,  G01N2446/00

摘要： An apparatus for measuring a target molecule in a sample is disclosed. The apparatus comprises a moiety comprising a magnetic label (1) greater than 100 nm, a binding surface (12) for specifically binding the moiety, the amount of said moiety binding to said surface being indicative of the amount of the target molecule in said sample, detection means (31 31′) for detecting the amount of said moiety bound to said surface, and a salt (51) for reducing aggregation of the magnetic labels of respective moieties in said sample. The apparatus preferably also comprises a magnetic field generator (41) for attracting the magnetic labels to the binding surface. A method for measuring a target molecule in a sample and a disposable cartridge for use with the apparatus are also disclosed.

摘要翻译： 公开了一种用于测量样品中靶分子的装置。 该装置包括包含大于100nm的磁性标签（1）的部分，用于特异性结合该部分的结合表面（12），所述部分结合所述表面的量指示所述样品中靶分子的量 ，用于检测与所述表面结合的所述部分的量的检测装置（31 31'）和用于减少所述样品中各部分的磁性标记物的聚集的盐（51）。 该装置优选地还包括用于将磁性标签吸引到装订表面的磁场发生器（41）。 还公开了一种用于测量样品中的靶分子的方法和用于该设备的一次性盒。

公开(公告)号：US20120171781A1

公开(公告)日：2012-07-05

申请号：US13496023

申请日：2010-09-07

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Marco Hendrikus Hefti ,  David Walterus Cornelis Dekkers ,  Michael Franciscus Wilhelmus Cornelis Martens

IPC分类号： G01N33/566 ,  G01N21/75 ,  G01N33/577

CPC分类号： G01N33/54346 ,  G01N33/54333 ,  G01N2333/58

摘要： The present invention is related to an immunoassay for the detection of an analyte in a sample, said assay comprising a plurality of moieties capable of binding to said analyte, wherein capture moieties which are not specific for the same epitope are bound to a solid substrate, and at least one epitope-specific detection moiety is bound to a detectable marker, and wherein the detectable marker to which the epitope-specific detection moiety is bound is a large particle marker having a particle size of ≧50 nm and ≦5000 nm.

摘要翻译： 本发明涉及用于检测样品中分析物的免疫测定法，所述测定法包括能够结合所述分析物的多个部分，其中对相同表位不特异的捕获部分结合到固体底物上， 并且至少一个表位特异性检测部分结合到可检测标记上，并且其中表位特异性检测部分结合的可检测标记是具有≥50nm和＆lt; NE; 5000nm的粒度的大颗粒标记物。

公开(公告)号：US09720003B2

公开(公告)日：2017-08-01

申请号：US13141439

申请日：2009-12-15

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

IPC分类号： G01N33/68 ,  G01N33/543

CPC分类号： G01N33/6887 ,  G01N33/54373 ,  G01N2800/324

摘要： The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

公开(公告)号：US20110256640A1

公开(公告)日：2011-10-20

申请号：US13141439

申请日：2009-12-15

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

IPC分类号： G01N33/566 ,  G01N30/00

CPC分类号： G01N33/6887 ,  G01N33/54373 ,  G01N2800/324

摘要： The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

摘要翻译： 本发明涉及一种测量样品中肌钙蛋白I的方法，包括以下步骤：提供样品，使样品与偶联至磁性标签的单克隆抗肌钙蛋白I抗体接触，使样品与多克隆抗肌钙蛋白I抗体 耦合到传感器表面并检测传感器表面上的磁性标签。 本发明还涉及用于测量样品中的肌钙蛋白I的装置和盒。

公开(公告)号：US20110207229A1

公开(公告)日：2011-08-25

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US09841421B2

公开(公告)日：2017-12-12

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus Dionisius Van Zon

IPC分类号： G01N33/543 ,  G01N27/82 ,  G01N27/72 ,  G01N27/74 ,  G01N35/00 ,  G01N21/27 ,  G01N21/552

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

公开(公告)号：US09023651B2

公开(公告)日：2015-05-05

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/553 ,  G01N33/543 ,  B82Y25/00 ,  G01N27/74 ,  G01R33/09 ,  G01R33/12 ,  G01N35/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US20140057366A1

公开(公告)日：2014-02-27

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

摘要翻译： 本发明涉及一种传感器装置（100）和用于在具有接触表面（11））的样品室（2）中检测磁性颗粒（1）的方法。 传感器装置（100）包括用于检测目标区域（TR）中和/或接触表面上的至少一个参考区域中的磁性颗粒（1）的传感器单元（120,130）。 此外，它包括用于产生磁场的磁场发生器（140），该磁场将磁性颗粒引导到接触表面。 在这些组件的帮助下，确定与磁性颗粒（1）和/或它们的运动相关的“辅助参数”，但是与磁性颗粒和接触表面之间的目标区域中发生的结合过程无关。 辅助参数可以例如与在磁场和目标区域（TR）的影响下由磁性颗粒（1）达到的位置之间的失配程度相关。 评估结果可用于验证和/或校正在目标区域（TR）中获得的测量结果。

公开(公告)号：US10041939B2

公开(公告)日：2018-08-07

申请号：US13497813

申请日：2010-09-10

申请人： Jeroen Hans Nieuwennhuis ,  Toon Hendrik Evers

发明人： Jeroen Hans Nieuwennhuis ,  Toon Hendrik Evers

IPC分类号： G01N33/553 ,  G01N33/543 ,  G01N27/74

摘要： The present invention relates to a cartridge (1) for the detection of an analyte (2) in a binding assay with magnetic labels (3), the cartridge comprising at least one capture binding agent (4) against a binding site on the analyte and comprising at least two magnetically labelled tracer binding agents (51 and 52) against further different binding sites on the analyte, characterized in that the cartridge comprises at least two regions (61 and 62) wherein a first region (61) comprises the at least one capture binding agent (4) and a first magnetically labelled tracer binding agent (51) and wherein a second region (62) comprises at least one capture binding agent (4) and a second magnetically labelled tracer binding agent (52). Herein the first region (61) does not comprise a magnetically labelled tracer binding agent other than the first magnetically labelled tracer bind agent (51) and wherein the second region (62) does not comprise a magnetically labelled tracer binding agent other than the second magnetically labelled tracer binding agent (52).