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    Magnetic immunochromatographic test method and device
    6.
    发明授权
    Magnetic immunochromatographic test method and device 有权
    磁免疫层析试验方法及装置

    公开(公告)号：US09329181B2

    公开(公告)日：2016-05-03

    申请号：US12293726

    申请日：2007-03-21

    申请人： Stephane Legastelois ,  Petr Ivanovich Nikitin ,  Jean-Marie Billiotte

    发明人： Stephane Legastelois ,  Petr Ivanovich Nikitin ,  Jean-Marie Billiotte

    IPC分类号： G01N33/558 ,  G01N33/58

    CPC分类号： G01N33/558 ,  G01N33/585 ,  G01N2446/00

    摘要： Method for detecting and quantifying an analyte in a liquid sample, using a test strip and magnetic particles as a detectable label and based on the detection of an amount of magnetic particles which become bound to a reading zone of a test strip as a result of performing the method, said amount being linked through a function to the analyte content of the sample, wherein the magnetic particles exhibit a nonlinear magnetization characteristic, which test strip is made of porous material and is arranged inside a case, cartridge or the like to form an assay device, wherein in its part surrounding the reading zone, the case is made of a material that is permeable to magnetic field, wherein for the reading of the reaction, the assay device is positioned in a measure cell of a magnetic reading device which detects the amount of magnetic particles in the reading zone by submitting it to at least one excitation magnetic field having about 90% of its power within one frequency band or a plurality of frequency bands, and the reading device measures the induced magnetic response field outside said frequency band or said plurality of frequency bands.

    摘要翻译： 用于检测和定量液体样品中的分析物的方法，使用测试条和磁性颗粒作为可检测标记，并且基于检测结合到测试条的读取区的磁性颗粒的量，作为执行结果 所述量通过功能与样品的分析物含量相关联，其中磁性颗粒表现出非线性磁化特性，该测试条由多孔材料制成并且布置在壳体，盒等内部以形成 测定装置，其中围绕读取区的部分，壳体由可渗透磁场的材料制成，其中为了读取反应，测定装置位于磁读取装置的测量单元中，该测量单元检测 读取区域中的磁性颗粒的量通过将其提供到在一个频带内具有大约90％的功率的至少一个激励磁场或多个 读取装置测量所述频带或所述多个频带之外的感应磁响应场。

    Detection of Specific Antigens in a Population of Antigens
    7.
    发明申请
    Detection of Specific Antigens in a Population of Antigens 审中-公开
    检测抗原人群中的具体抗原

    公开(公告)号：US20160069877A1

    公开(公告)日：2016-03-10

    申请号：US14756975

    申请日：2015-11-04

    申请人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

    发明人： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56966 ,  G01N33/5304 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54326 ,  G01N33/80 ,  G01N2333/705 ,  G01N2446/00

    摘要： Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH).The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique.Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition.Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.

    摘要翻译： 用于检测在具有Rh阴性细胞的混合群体中的至少两组细胞特别是Rh阳性细胞的混合细胞群中的一组细胞的存在或不存在并用于定量的方法，如在胎儿母体出血中发现的 （FMH）。 用抗D抗体包被的磁性颗粒与Rh阴性母体血液中的Rh阳性胎儿细胞反应，然后进行特异性分离和定量技术。 重力或磁力用于移动反应的磁性颗粒以分离，区分和定量由抗原组成分化的细胞。 Rh阳性细胞体积与原始血液样品的体积相关，作为需要施用于母亲以防止随后的Rh免疫的RhIG剂量数量的指示。

    SIMULTANEOUS ASSAY FOR DETERMINING DRUGS
    9.
    发明申请
    SIMULTANEOUS ASSAY FOR DETERMINING DRUGS 有权
    同时测定药物的同时测定

    公开(公告)号：US20110190169A1

    公开(公告)日：2011-08-04

    申请号：US13086229

    申请日：2011-04-13

    申请人： William F. Link ,  Renato B. del Rosario ,  Randy V. Sweet ,  David L. King

    发明人： William F. Link ,  Renato B. del Rosario ,  Randy V. Sweet ,  David L. King

    IPC分类号： C40B40/10

    CPC分类号： G01N33/54326 ,  G01N33/94 ,  G01N2446/00 ,  Y10T436/101666 ,  Y10T436/108331 ,  Y10T436/25125

    摘要： Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies. In an alternative procedure, the added drugs are not coupled to the microparticles but are pre-labeled, while the antibodies are pre-coupled to the microparticles, and the assay proceeds without further incubation. In both alternatives, the microparticles are ultimately recovered from the assay medium and from any unbound species, and the recovered microparticles are analyzed by flow cytometry to obtain indications of the presence of the various drugs in the sample in an inverse manner by detection of the label, each drug differentiable from the others by the distinguishing features of the microparticles.

    摘要翻译： 在同时测定中分析体液中所选择的药物组的药物的存在，其中将流体样品与附加量的所有药物，面板每种药物特异的抗体和微粒 ，将微粒分为子集，面板中每种药物的一个子集，以及与其他子集可区分的每个子集。 孵育在其中发生竞争性结合的液体培养基中进行，样品中的药物与添加到测定培养基中的药物与抗体结合。 在一个程序中，将所添加的药物预先偶联到微粒，而不是抗体，然后孵育后，用对抗体具有亲和性的标记配体进一步孵育微粒。 在替代方法中，所添加的药物不与微粒偶联，而是预先标记，而抗体预偶联于微粒，并且测定在不进一步孵育的情况下进行。 在两种替代方案中，微粒最终从测定培养基和任何未结合物质中回收，并且通过流式细胞术分析回收的微粒，以通过检测标签以相反的方式获得样品中各种药物的存在的迹象 ，每种药物通过微粒的区别特征与其他药物不同。

    Method and apparatus for imaging target components in a biological sample using permanent magnets
    10.
    发明申请
    Method and apparatus for imaging target components in a biological sample using permanent magnets 审中-公开
    用于使用永磁体对生物样品中的目标成分进行成像的方法和装置

    公开(公告)号：US20110014686A1

    公开(公告)日：2011-01-20

    申请号：US12888562

    申请日：2010-09-23

    申请人： Arjan G.J. Tibbe ,  Leon W.M.M. Terstappen

    发明人： Arjan G.J. Tibbe ,  Leon W.M.M. Terstappen

    IPC分类号： C12M1/42

    CPC分类号： G01N33/582 ,  G01N33/54326 ,  G01N33/56972 ,  G01N2333/70514 ,  G01N2446/00

    摘要： A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution. Image analysis can be performed with a novel algorithm to provide a count of the cells on the surface, reflecting the target cell concentration of the original sample.

    摘要翻译： 描述了通过图像细胞计数法检测流体细胞的系统，用于评估目标群体，例如不同体液中的白细胞亚群或环境样品，食品和体液中的细菌污染物。 简言之，荧光标记的靶细胞与磁性颗粒或珠粒连接。 在一个实施例中，将小的永磁体直接插入到包含标记电池的腔室中。 这些磁体用PDMS硅橡胶涂覆，以提供平滑和均匀的表面，允许在单个焦平面上进行成像。 从样品中除去磁体，并用由CCD照相机俘获的靶细胞发射的荧光照射。 在另一个实施例中，具有永磁体的浮子允许靶细胞沿着样品溶液内的单个成像平面排列。 可以用新颖的算法进行图像分析，以提供表面上的细胞计数，反映原始样品的靶细胞浓度。