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1.发明申请Nucleic Acid Sequences That Can Be Used As Primers And Probes In The Amplification And Detection Of Sars Coronavirus 失效可用于引物和探针的核酸序列在Sars冠状病毒的扩增和检测中公开(公告)号：US20090226885A1
公开(公告)日：2009-09-10
申请号：US10559949
申请日：2004-03-08
Applicant: P.T.G Sillekens , Marlieke Overdijk , Saskia van de Laar
Inventor： P.T.G Sillekens , Marlieke Overdijk , Saskia van de Laar
CPC classification number: C12Q1/701 , C12Q2531/113 , C12Q2531/143 , C12Q2561/101
Abstract: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with a novel human coronavirus causing Severe Acute Respiratory Syndrome (SARS). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of SARS nucleic acid. The oligonucleotide sequences provided with the present invention are located in the replicase gene, the nucleocapsid gene and the 3′ end non-coding region of the SARS Coronavirus genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of SARS Coronavirus can be obtained. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
Abstract translation: 本发明涉及可用于病毒诊断领域的核酸序列，更具体地说，涉及引起严重急性呼吸综合征（SARS）的新型人冠状病毒感染的诊断。 本发明提供了可用作扩增和检测SARS核酸的引物和探针的核苷酸序列。 本发明提供的寡核苷酸序列位于SARS冠状病毒基因组的复制酶基因，核衣壳基因和3'端非编码区。 已经发现，通过将本发明的序列用于扩增和检测核酸的方法，可以获得SARS的敏感和特异性检测冠状病毒。 根据本发明的寡核苷酸序列特别可用于扩增核酸的方法。
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2.发明授权Nucleic acid sequences that can be used as primers and probes in the amplification and detection of SARS coronavirus 失效可用作扩增和检测SARS冠状病毒的引物和探针的核酸序列公开(公告)号：US08106172B2
公开(公告)日：2012-01-31
申请号：US10559949
申请日：2004-03-08
Applicant: Peter T. G. Sillekens , Marlieke Overdijk , Saskia van de Laar
Inventor： Peter T. G. Sillekens , Marlieke Overdijk , Saskia van de Laar
CPC classification number: C12Q1/701 , C12Q2531/113 , C12Q2531/143 , C12Q2561/101
Abstract: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with a novel human coronavirus causing Severe Acute Respiratory Syndrome (SARS). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of SARS nucleic acid. The oligonucleotide sequences provided with the present invention are located in the replicase gene, the nucleocapsid gene and the 3′ end non-coding region of the SARS Coronavirus genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of SARS Coronavirus can be obtained. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
Abstract translation: 本发明涉及可用于病毒诊断领域的核酸序列，更具体地说，涉及引起严重急性呼吸综合征（SARS）的新型人冠状病毒感染的诊断。 本发明提供了可用作扩增和检测SARS核酸的引物和探针的核苷酸序列。 本发明提供的寡核苷酸序列位于SARS冠状病毒基因组的复制酶基因，核衣壳基因和3'端非编码区。 已经发现，通过将本发明的序列用于扩增和检测核酸的方法，可以获得SARS的敏感和特异性检测冠状病毒。 根据本发明的寡核苷酸序列特别可用于扩增核酸的方法。
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公开(公告)号：US09005896B2
公开(公告)日：2015-04-14
申请号：US13818325
申请日：2011-08-31
Applicant: Harma Feitsma , Marlieke Overdijk , Anja Van De Stople , Pieter Jan Van Der Zaag
Inventor： Harma Feitsma , Marlieke Overdijk , Anja Van De Stople , Pieter Jan Van Der Zaag
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2525/301
Abstract: The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridize to capture probe nucleic acids which are immobilized to a support via their 5′ end. The hybridization product is further extended with a polymerase to form a double stranded nucleic acid. To this double stranded nucleic acid, a hairpin nucleic acid is ligated. This ligation product is further amplified and sequenced.
Abstract translation: 本发明描述了用于扩增靶核酸的方法，其中靶核酸与捕获通过其5'末端固定在载体上的探针核酸杂交。 进一步用聚合酶扩增杂交产物以形成双链核酸。 向该双链核酸中连接发夹核酸。 该连接产物进一步扩增和测序。
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公开(公告)号：US20130210008A1
公开(公告)日：2013-08-15
申请号：US13818325
申请日：2011-08-31
Applicant: Harma Feitsma , Marlieke Overdijk , Anja Van De Stople , Pieter Jan Van Der Zaag
Inventor： Harma Feitsma , Marlieke Overdijk , Anja Van De Stople , Pieter Jan Van Der Zaag
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2525/301
Abstract: The present invention describes methods for amplifying a target nucleic acid wherein target nucleic acids hybridise to capture probe nucleic acids which are immobilized to a support via their 5′ end. The hybridization product is further extended with a polymerase to form a double stranded nucleic acid. To this double stranded nucleic acid, a hairpin nucleic acid is ligated. This ligation product is further amplified and sequenced.
Abstract translation: 本发明描述了用于扩增目标核酸的方法，其中靶核酸与通过其5'末端固定在载体上的探针核酸杂交。 进一步用聚合酶扩增杂交产物以形成双链核酸。 向该双链核酸中连接发夹核酸。 该连接产物进一步扩增和测序。
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