公开(公告)号：US20160115459A1

公开(公告)日：2016-04-28

申请号：US14894484

申请日：2014-05-26

申请人： Martín Diego Linus SPATZ ,  INMUNOVA S.A.

发明人： Fernando Alberto GOLDBAUM ,  Vanesa ZYLBERMAN ,  Patricio Oliver CRAIG ,  Giselle GHERSI ,  Marina Sandra PALERMO ,  Maria Pilar MEJIAS ,  Leticia Veronica BENTANCOR

IPC分类号： C12N9/10 ,  C07K16/12 ,  A61K38/45 ,  C07K16/40 ,  C07K14/245 ,  A61K39/108

CPC分类号： C12N9/1085 ,  A61K38/00 ,  A61K38/45 ,  A61K39/0258 ,  A61K39/098 ,  A61K2039/505 ,  A61K2039/523 ,  A61K2039/545 ,  A61K2039/55505 ,  A61K2039/55566 ,  A61K2039/575 ,  A61K2039/6037 ,  A61K2039/6068 ,  C07K14/245 ,  C07K16/1232 ,  C07K16/18 ,  C07K16/40 ,  C07K2317/20 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2319/55 ,  C12Y205/01078 ,  Y02A50/474

摘要： Chimeric polypeptides useful as immunogens for inducing protective immune responses and neutralizing antibodies against Shiga toxin (Stx) in mammals. More specifically, chimeric polypeptides having a monomer of the homopentameric B subunit of the Shiga 2 toxin fused to the N-terminus of a monomer of Brucella lumazine synthase, and to oligomeric protein complexes formed from said chimeric polypeptides. Polynucleotides and vectors encoding said chimeric polypeptides, transgenic cells having said polynucleotides and vectors, pharmaceutical compositions, such as a vaccine, having said chimeric polypeptides and chimeric polynucleotides, antibodies which bind to the chimeric polypeptides, a method for obtaining antibodies which bind specifically to the subunit B of the Shiga 2 toxin and methods of lowering the bacterial load of enterohemorrhagic Escherichia coli in mammals, which can be used for prevention of inter cilia, hemolytic-ureic syndrome (HUS), are also provided.

摘要翻译： 嵌合多肽可用作诱导保护性免疫应答的免疫原并中和哺乳动物志贺毒素（Stx）的抗体。 更具体地，具有与Brucella lumazine合酶的单体的N末端融合的志贺二毒素的同基因聚合物B亚基的单体的嵌合多肽和由所述嵌合多肽形成的寡聚蛋白复合物。 编码所述嵌合多肽的多核苷酸和载体，具有所述多核苷酸和载体的转基因细胞，具有所述嵌合多肽和嵌合多核苷酸的药物组合物，例如疫苗，与嵌合多肽结合的抗体，获得与 也提供了滋贺2毒素的亚基B和降低哺乳动物肠出血性大肠杆菌的细菌负荷的方法，其可用于预防纤维蛋白溶解性尿毒症综合征（HUS）。