公开(公告)号：US5824790A

公开(公告)日：1998-10-20

申请号：US572951

申请日：1995-12-15

申请人： Peter Lewis Keeling ,  Mary E. Knight ,  Hanping Guan

发明人： Peter Lewis Keeling ,  Mary E. Knight ,  Hanping Guan

IPC分类号： A01H5/10 ,  A21D2/18 ,  A23G1/00 ,  A23G1/42 ,  A23G1/56 ,  A23G3/00 ,  A23G3/34 ,  A23G3/36 ,  A23L1/0522 ,  C12N15/05 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  C12N15/29

CPC分类号： A01H5/10 ,  A21D2/186 ,  A23G1/423 ,  A23G1/56 ,  A23G3/346 ,  A23G3/366 ,  A23L29/212 ,  C12N15/8245 ,  A23G2200/06

摘要： A method of producing a plant with novel starch-synthesising ability comprises stably incorporating into the genome of a recipient plant at least one target gene encoding an enzyme involved in a starch or glycogen biosynthetic pathway. A plant with novel starch-synthesising ability may have novel starch quality (eg altered fine structure). Starch or glycogen biosynthetic enzymes include soluble starch synthase, branching enzyme, glycogen synthase, ADP-glucose pyrophosphorylase, self-glucosylating protein, glycogenin and amylogenin. Mutants involving said enzymes for use in this method are described, including specific gene-dosage combinations which provide novel starch fine structures and starch properties. DNA constructs for use in this method are described, as well as plants transformed with said DNA constructs, the seeds and progeny of such plants, and hybrids whose pedigree includes such plants.

摘要翻译： 制备具有新型淀粉合成能力的植物的方法包括将至少一种编码参与淀粉或糖原生物合成途径的酶的靶基因稳定地并入受体植物的基因组中。 具有新型淀粉合成能力的植物可能具有新颖的淀粉质量（例如改变的精细结构）。 淀粉或糖原生物合成酶包括可溶性淀粉合成酶，支化酶，糖原合成酶，ADP-葡萄糖焦磷酸化酶，自身葡萄糖基化蛋白，糖原素和代谢酶。 描述了涉及用于该方法的所述酶的突变体，包括提供新型淀粉精细结构和淀粉性质的特定基因 - 剂量组合。 描述了用于该方法的DNA构建体，以及用所述DNA构建体，这种植物的种子和子代转化的植物，以及其谱系包括这种植物的杂种。