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    Modification of starch synthesis in plants
    1.
    发明授权
    Modification of starch synthesis in plants 失效
    植物中淀粉合成的修饰

    公开(公告)号：US5824790A

    公开(公告)日：1998-10-20

    申请号：US572951

    申请日：1995-12-15

    申请人： Peter Lewis Keeling ,  Mary E. Knight ,  Hanping Guan

    发明人： Peter Lewis Keeling ,  Mary E. Knight ,  Hanping Guan

    IPC分类号： A01H5/10 ,  A21D2/18 ,  A23G1/00 ,  A23G1/42 ,  A23G1/56 ,  A23G3/00 ,  A23G3/34 ,  A23G3/36 ,  A23L1/0522 ,  C12N15/05 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  C12N15/29

    CPC分类号： A01H5/10 ,  A21D2/186 ,  A23G1/423 ,  A23G1/56 ,  A23G3/346 ,  A23G3/366 ,  A23L29/212 ,  C12N15/8245 ,  A23G2200/06

    摘要： A method of producing a plant with novel starch-synthesising ability comprises stably incorporating into the genome of a recipient plant at least one target gene encoding an enzyme involved in a starch or glycogen biosynthetic pathway. A plant with novel starch-synthesising ability may have novel starch quality (eg altered fine structure). Starch or glycogen biosynthetic enzymes include soluble starch synthase, branching enzyme, glycogen synthase, ADP-glucose pyrophosphorylase, self-glucosylating protein, glycogenin and amylogenin. Mutants involving said enzymes for use in this method are described, including specific gene-dosage combinations which provide novel starch fine structures and starch properties. DNA constructs for use in this method are described, as well as plants transformed with said DNA constructs, the seeds and progeny of such plants, and hybrids whose pedigree includes such plants.

    摘要翻译： 制备具有新型淀粉合成能力的植物的方法包括将至少一种编码参与淀粉或糖原生物合成途径的酶的靶基因稳定地并入受体植物的基因组中。 具有新型淀粉合成能力的植物可能具有新颖的淀粉质量（例如改变的精细结构）。 淀粉或糖原生物合成酶包括可溶性淀粉合成酶，支化酶，糖原合成酶，ADP-葡萄糖焦磷酸化酶，自身葡萄糖基化蛋白，糖原素和代谢酶。 描述了涉及用于该方法的所述酶的突变体，包括提供新型淀粉精细结构和淀粉性质的特定基因 - 剂量组合。 描述了用于该方法的DNA构建体，以及用所述DNA构建体，这种植物的种子和子代转化的植物，以及其谱系包括这种植物的杂种。

    Starch encapsulation
    2.
    发明授权
    Starch encapsulation 失效
    淀粉封装

    公开(公告)号：US6107060A

    公开(公告)日：2000-08-22

    申请号：US941445

    申请日：1997-09-30

    申请人： Peter Keeling ,  Hanping Guan

    发明人： Peter Keeling ,  Hanping Guan

    IPC分类号： A01H1/00 ,  C12N1/21 ,  C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/82 ,  C12N15/62 ,  C12N15/63 ,  C12N15/70

    CPC分类号： C12N15/8251 ,  C12N15/8245 ,  C12N9/1048 ,  C07K2319/00

    摘要： Hybrid polypeptides are provided formed with encapsulating regions from genes that encode for anabolic proteins. More particularly, the present invention relates to recombinant nucleic acid molecules that code for genes which encapsulate an attached protein within a matrix; preferably, these genes encapsulate a desired ("payload") polypeptide within starch, and more specifically within the starch granule matrix. Expression vectors comprising these recombinant nucleic acid molecules, and hosts therefor, and more specifically the starch-bearing portions of such hosts, transformed with such vectors, are also provided. Preferably, grain containing a foreign protein encapsulated within the starch is provided, useful to produce mammalian, fish and avian food. The invention also encompasses methods of producing purified protein from starch and particularly from starch granules, and industrial uses of such protein.

    摘要翻译： 提供了从编码合成代谢蛋白的基因的包封区形成杂交多肽。 更具体地，本发明涉及编码将附着的蛋白质包封在基质内的基因的重组核酸分子; 优选地，这些基因将淀粉内的期望（“有效载荷”）多肽包封，更具体地在淀粉颗粒基质内包封。 还提供了包含这些重组核酸分子及其宿主的表达载体，更具体地，用这些载体转化的这种宿主的含淀粉部分。 优选地，提供了包含在淀粉内的外来蛋白质的谷粒，可用于产生哺乳动物，鱼和禽类食物。 本发明还包括从淀粉，特别是淀粉颗粒产生纯化蛋白质的方法和这种蛋白质的工业用途。

    METHODS OF INCREASING PROTEIN, OIL, AND/OR AMINO ACID CONTENT IN A PLANT
    4.
    发明申请
    METHODS OF INCREASING PROTEIN, OIL, AND/OR AMINO ACID CONTENT IN A PLANT 审中-公开
    在植物中增加蛋白质，油和/或氨基酸含量的方法

    公开(公告)号：US20130045324A1

    公开(公告)日：2013-02-21

    申请号：US13587074

    申请日：2012-08-16

    申请人： Hanping Guan ,  Heiko Härtel ,  Suzy M. Cocciolone

    发明人： Hanping Guan ,  Heiko Härtel ,  Suzy M. Cocciolone

    IPC分类号： A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  A23L1/10 ,  A23L1/212 ,  A23K1/00 ,  A23L1/307 ,  A23K1/18 ,  C12N15/82 ,  A01H1/02

    CPC分类号： C12N15/8251 ,  A23K20/142 ,  A23K20/147 ,  A23K20/158 ,  A23L7/10 ,  C12N9/1051 ,  C12N15/8247

    摘要： This invention relates generally to methods for preparing plants, plant cells, or plant parts with increased content in one or more of protein, oil, or one or more amino acids. The invention also relates to transgenic plants comprising multiple genes of interest including a nucleic acid molecule encoding a polypeptide having pyruvate kinase activity and a nucleic acid molecule encoding a trehalose-6-phosphate synthase (TPS) homolog. Expression cassettes for achieving such gene expression manipulation, as well as recombinant constructs, vectors and plants, plant cells, or plant parts comprising the same, are also provided. Plants, plant cells, or plant parts with increased content in one or more of protein, oil, or one or more amino acids thus obtained may be useful in preparing foodstuffs and animal feeds and in plant breeding programs for developing further hybrid or inbred lines.

    摘要翻译： 本发明一般涉及在蛋白质，油或一种或多种氨基酸中的一种或多种中增加含量的植物，植物细胞或植物部分的制备方法。 本发明还涉及包含感兴趣的多个基因的转基因植物，包括编码具有丙酮酸激酶活性的多肽的核酸分子和编码海藻糖-6-磷酸合成酶（TPS）同源物的核酸分子。 还提供了用于实现这种基因表达操纵的表达盒，以及重组构建体，载体和植物，植物细胞或包含其的植物部分。 在如此获得的一种或多种蛋白质，油或一种或多种氨基酸中增加含量的植物，植物细胞或植物部分可用于制备食物和动物饲料以及用于开发进一步杂种或近交系的植物育种程序。

    Transgenic corn having enhanced nutritional qualities
    5.
    发明授权
    Transgenic corn having enhanced nutritional qualities 失效
    具有增强营养品质的转基因玉米

    公开(公告)号：US08030540B2

    公开(公告)日：2011-10-04

    申请号：US10907936

    申请日：2005-04-21

    申请人： Hanping Guan ,  Deborah Wetterberg ,  Angela L. McKean ,  Peter L. Keeling

    发明人： Hanping Guan ,  Deborah Wetterberg ,  Angela L. McKean ,  Peter L. Keeling

    IPC分类号： C12N15/82 ,  C12N15/63 ,  C12N15/31 ,  A01H5/10 ,  A01H5/00 ,  A01H3/00

    CPC分类号： C12N15/8247 ,  C12N9/1241 ,  C12N15/8251 ,  C12N15/8261 ,  Y02A40/146

    摘要： The invention provides transgenic corn seed, which expresses a gene encoding a double mutant of the E. coli glgC gene in endosperm plastids, wherein the mutant protein has a proline to aspartic acid substitution at amino acid 295 and a glutamic acid to lysine substitution at amino acid 296. The transgenic corn seed of the invention is characterized by enhanced levels of a number of amino acids and oil, when compared to isogenic corn seed, which does not express the transgene in an endosperm plastid. However, the amount of starch in the transgenic corn seed of the invention is decreased or unchanged when compared to the amount of starch in the isogenic control corn seed.

    摘要翻译： 本发明提供了转基因玉米种子，其表达编码胚乳质体中大肠杆菌glgC基因的双重突变体的基因，其中突变蛋白质具有在氨基酸295处天冬氨酸取代的脯氨酸，并且在氨基酸处具有谷氨酸至赖氨酸取代 酸296.当与在胚乳质体中不表达转基因的等基因玉米种子相比，本发明的转基因玉米种子的特征在于增加多种氨基酸和油的水平。 然而，与同基因对照玉米种子中的淀粉的量相比，本发明的转基因玉米种子中的淀粉的量减少或不变。

    POLYNUCLEOTIDES FOR REGULATION OF HIGH LEVEL TISSUE-PREFERRED EXPRESSION IN CROP PLANTS
    6.
    发明申请
    POLYNUCLEOTIDES FOR REGULATION OF HIGH LEVEL TISSUE-PREFERRED EXPRESSION IN CROP PLANTS 审中-公开
    用于调控作物植物中高水平组织优先表达的多核苷酸

    公开(公告)号：US20100043100A1

    公开(公告)日：2010-02-18

    申请号：US12595276

    申请日：2008-04-07

    申请人： Christopher Kafer ,  Hanping Guan

    发明人： Christopher Kafer ,  Hanping Guan

    IPC分类号： A01H1/00 ,  C07H21/04 ,  A01H5/00 ,  C12N15/63 ,  C12N5/10

    CPC分类号： C12N15/8234

    摘要： This invention provides polynucleotides regulating high level tissue-preferred expression. Compositions comprising the polynucleotides include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the tissue-preferred regulatory elements.

    摘要翻译： 本发明提供调节高水平组织优选表达的多核苷酸。 包含多核苷酸的组合物包括可用于植物转化的DNA构建体和用这种DNA构建体转化的植物。 还提供了使用组织优选的调节元件在植物中表达转基因的方法。

    METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY
    7.
    发明申请
    METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY 审中-公开
    修饰木质素生物合成和改善可溶性的方法

    公开(公告)号：US20120272406A1

    公开(公告)日：2012-10-25

    申请号：US13434035

    申请日：2012-03-29

    申请人： Hanping Guan ,  Beomseok Seo ,  Peifeng Ren ,  David Deppong ,  Huihua Fu

    发明人： Hanping Guan ,  Beomseok Seo ,  Peifeng Ren ,  David Deppong ,  Huihua Fu

    IPC分类号： C12N15/82 ,  C12N5/10 ,  C12N1/13 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/113 ,  A01H5/00

    CPC分类号： C12N9/1011 ,  C12N15/8255 ,  C12Y201/01104

    摘要： The present invention relates to methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.

    摘要翻译： 本发明涉及通过改变植物的木质素生物合成来提高植物的消化率和/或粗蛋白质的方法。 该方法涉及通过使用miRNA直接降低基因的表达或使用转录因子的调节来操纵木质素生物合成途径中基因的表达水平。 还提供了用于实现这种基因表达操纵的表达盒和包含构建体和盒的转基因植物细胞和植物。 还开发了用于表达盒的转录调节核苷酸序列以及使用这些序列和盒的方法。

    Methods in Increasing Grain Value by Improving Grain Yield and Quality
    8.
    发明申请
    Methods in Increasing Grain Value by Improving Grain Yield and Quality 审中-公开
    提高粮食产量和质量提高粮食价值的方法

    公开(公告)号：US20120216315A1

    公开(公告)日：2012-08-23

    申请号：US12996020

    申请日：2009-06-10

    申请人： Hanping Guan ,  Beomseok Seo

    发明人： Hanping Guan ,  Beomseok Seo

    IPC分类号： A01H5/00 ,  A01H1/06 ,  A01H5/10 ,  C12N15/82

    CPC分类号： C12N15/8262 ,  C12N9/1025

    摘要： The invention provides a transgenic plant, which expresses a transgene encoding a citrate synthase (CS) wherein the transgenic plant seed of the invention is characterized by increased yield and/or enhanced levels of protein, essential amino acids or oil, when compared to an isoline that does not express the transgene; and also provides methods of producing transgenic plants with economically relevant traits and provides expression vectors comprising polynucleotides encoding Citrate Synthase.

    摘要翻译： 本发明提供了一种转基因植物，其表达编码柠檬酸合酶（CS）的转基因，其中本发明的转基因植物种子的特征在于与等位基因相比增加的产量和/或增加的蛋白质，必需氨基酸或油的水平 不表达转基因; 并且还提供生产具有经济相关性状的转基因植物的方法，并提供包含编码柠檬酸盐合酶的多核苷酸的表达载体。

    Starch encapsulation
    9.
    发明授权
    Starch encapsulation 失效
    淀粉封装

    公开(公告)号：US07141659B2

    公开(公告)日：2006-11-28

    申请号：US10628525

    申请日：2003-07-28

    申请人： Peter Keeling ,  Hanping Guan

    发明人： Peter Keeling ,  Hanping Guan

    IPC分类号： C07H21/04 ,  A61K49/14 ,  C12P19/14

    CPC分类号： C12N15/8251 ,  C07K2319/00 ,  C12N9/1048 ,  C12N15/8245

    摘要： The present invention relates to a recombinant nucleic acid encoding a hybrid polypeptide which comprises a transit peptide for the translocation of the polypeptide into an appropriate organelles such as plastids, a starch-encapsulating region from maize starch synthase and a payload polypeptide, wherein said payload polypeptide can be either N- or C-terminal to the starch encapsulating region. The invention also relates to the expression vectors comprising said nucleic acid, and hosts comprising the said vector. Also, the invention encompasses methods of producing the hybrid polypeptide thereof from starch and particularly from starch granules, and industrial uses of the payload polypeptide recombinantly produced in said hybrid polypeptide wherein said payload polypeptide is a biologically active molecule.

    摘要翻译： 本发明涉及编码杂合多肽的重组核酸，其包含用于将多肽转运到合适的细胞器中的转运肽，例如质体，来自玉米淀粉合成酶的淀粉包封区和有效载荷多肽，其中所述有效载荷多肽 可以是淀粉包封区域的N-或C-末端。 本发明还涉及包含所述核酸的表达载体和包含所述载体的宿主。 此外，本发明包括从淀粉，特别是淀粉颗粒产生其杂合多肽的方法，以及在所述杂合多肽中重组产生的有效载荷多肽的工业用途，其中所述有效载荷多肽是生物活性分子。