公开(公告)号：US6107471A

公开(公告)日：2000-08-22

申请号：US410028

申请日：1999-10-01

申请人： Masaomi Arahira ,  Chikafusa Fukazawa

发明人： Masaomi Arahira ,  Chikafusa Fukazawa

IPC分类号： C12N15/09 ,  C12N9/50 ,  C12N9/64 ,  C12N15/00 ,  C12N15/29 ,  C12N15/57 ,  C12R1/91

CPC分类号： C12N9/6478

摘要： Disclosed are a cDNA encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 1 in the sequence list and a gene encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 2 in the sequence listing. The enzymes enable production of asparagine residue-specific endoprotease in large amounts.

摘要翻译： 公开了编码具有序列表中SEQ ID NO：1所示的氨基序列的植物来源的天冬酰胺残基特异性内切蛋白酶的cDNA，以及编码具有氨基酸序列的植物来源的天冬酰胺残基特异性内切蛋白酶的基因 序列表中的SEQ ID NO：2。 这些酶能够大量生产天冬酰胺残基特异性内切蛋白酶。

公开(公告)号：US4871840A

公开(公告)日：1989-10-03

申请号：US151805

申请日：1988-02-03

申请人： Shoichi Kobayashi ,  Masaomi Arahira

发明人： Shoichi Kobayashi ,  Masaomi Arahira

IPC分类号： A23L1/03 ,  A23L1/308 ,  A61K8/72 ,  A61K8/73 ,  A61K47/40 ,  C08B37/16 ,  C12N1/38 ,  C12P19/16

CPC分类号： C08B37/0012 ,  C12P19/16

摘要： Different from conventional heterogeneous multiple-branched cyclodextrins having branches derived from the same kind of saccharide molecule, the inventive heterogeneous multiple-branched cyclodextrin has branches derived from different kinds of saccharides of, one, glucose and, the other, a maltooligosaccharide. The inventive heterogeneous multiple-branched cyclodextrins are more stable than conventional ones and useful as an ingredient of medical, foodstuff and cosmetic preparations. The heterogeneous multiple-branched cyclodextrin can be prepared from a mixture of a maltooligosaccharide and a glucosyl cyclodextrin by the reverse action of debranching enzyme.

公开(公告)号：US06492114B2

公开(公告)日：2002-12-10

申请号：US09494438

申请日：2000-01-31

申请人： Masaomi Arahira ,  Chikafusa Fukazawa

发明人： Masaomi Arahira ,  Chikafusa Fukazawa

IPC分类号： C12Q168

CPC分类号： C07H21/00 ,  C12N15/1003 ,  Y10T436/143333

摘要： Disclosed is a method for preparing a template DNA from a processed vegetable food, comprising the steps of: extracting a crude DNA fraction from a processed vegetable food subjected to at least one of a high temperature treatment, a high temperature grinding treatment, a high pressure treatment and a fermentation treatment, and optionally subjected to defatting; and performing a DNA fractionation treatment of the crude DNA fraction by a polyethylene glycol precipitation method and/or a polyacrylamide gel electrophoresis method. Also disclosed is a method for detecting a gene of a plant in a processed vegetable food derived from the plant by a PCR method in which such a template DNA is used. The processed vegetable food may be an oil source seed.

摘要翻译： 本发明公开了一种从加工蔬菜食品中制备模板DNA的方法，包括以下步骤：从经过高温处理，高温研磨处理，高压 处理和发酵处理，任选地进行脱脂; 并通过聚乙二醇沉淀法和/或聚丙烯酰胺凝胶电泳法对粗制DNA部分进行DNA分级处理。 还公开了通过使用这样的模板DNA的PCR法检测来自植物的加工植物性食品中的植物的基因的方法。 经处理的蔬菜食品可以是油源种子。

公开(公告)号：US06372469B1

公开(公告)日：2002-04-16

申请号：US09412600

申请日：1999-10-06

申请人： Masaomi Arahira ,  Chikafusa Fukazawa

发明人： Masaomi Arahira ,  Chikafusa Fukazawa

IPC分类号： C12N912

CPC分类号： C12N9/14

摘要： Isolation of a cDNA encoding a plant-derived epoxide hydrolase and an expression system of the enzyme in Escherichia coli are established, so as to permit the mass-scale production of the plant-derived epoxide hydrolase. The invention provides a cDNA encoding the plant-derived epoxide hydrolase having the amino acid sequence of SQ ID No. 1 in the Sequence Listing, a gene encoding the plant-derived epoxide hydrolase having the amino acid sequence of SQ ID No. 2 in the Sequence Listing, a plasmid vector carrying the cDNA and the transformant (FERM BP-6624) retaining the plasmid vector.

摘要翻译： 建立了编码植物来源的环氧化物水解酶的cDNA和大肠杆菌中的酶的表达系统的分离，从而允许大规模生产植物来源的环氧化物水解酶。 本发明提供了编码植物来源的环氧化物水解酶的cDNA，该水解酶具有序列表中的SQID No.1序列的氨基酸序列，该基因编码植物来源的环氧化物水解酶，该氨基酸序列具有SEQ ID No.2的氨基酸序列 序列表，携带有保留质粒载体的cDNA和转化体（FERM BP-6624）的质粒载体。

公开(公告)号：US06251623B1

公开(公告)日：2001-06-26

申请号：US09410025

申请日：1999-10-01

申请人： Masaomi Arahira ,  Chikafusa Fukazawa

发明人： Masaomi Arahira ,  Chikafusa Fukazawa

IPC分类号： C12Q137

CPC分类号： C12Q1/37 ,  Y10S435/814

摘要： A quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease is disclosed which comprises measuring fluorescence generated by a fluorescence quenching substrate split by an asparagine residue-specific endoprotease. The fluorescence quenching substrate comprises an oligopeptide having in its amino acid sequence at least one asparagine residue, whose C-terminal side is other than an isoleucine residue, a leucine residue, or a valine residue, wherein the oligopeptide has a 7-methoxycoumarin-4-yl-acetyl group arranged on its N-terminal side and a 2,4-dinitrophenyl group arranged on its C-terminal side.

摘要翻译： 公开了一种用于植物衍生的天冬酰胺残基特异性内切蛋白酶活性的快速测定方法，其包括测量由天冬酰胺残基特异性内切蛋白酶分裂的荧光猝灭底物产生的荧光。 荧光猝灭底物包含在其氨基酸序列中具有至少一个天冬酰胺残基的寡肽，其C末端侧不是异亮氨酸残基，亮氨酸残基或缬氨酸残基，其中寡肽具有7-甲氧基香豆素-4 - 酰基乙酰基和其C-末端侧排列的2,4-二硝基苯基。