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1.发明授权
公开(公告)号：US4871840A
公开(公告)日：1989-10-03
申请号：US151805
申请日：1988-02-03
申请人： Shoichi Kobayashi , Masaomi Arahira
发明人： Shoichi Kobayashi , Masaomi Arahira
CPC分类号： C08B37/0012 , C12P19/16
摘要： Different from conventional heterogeneous multiple-branched cyclodextrins having branches derived from the same kind of saccharide molecule, the inventive heterogeneous multiple-branched cyclodextrin has branches derived from different kinds of saccharides of, one, glucose and, the other, a maltooligosaccharide. The inventive heterogeneous multiple-branched cyclodextrins are more stable than conventional ones and useful as an ingredient of medical, foodstuff and cosmetic preparations. The heterogeneous multiple-branched cyclodextrin can be prepared from a mixture of a maltooligosaccharide and a glucosyl cyclodextrin by the reverse action of debranching enzyme.
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2.发明授权Plant-derived, asparagine residue-specific endoprotease cDNA and a gene 失效植物来源的天冬酰胺残基特异性内切蛋白酶cDNA和基因
公开(公告)号：US6107471A
公开(公告)日：2000-08-22
申请号：US410028
申请日：1999-10-01
CPC分类号： C12N9/6478
摘要： Disclosed are a cDNA encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 1 in the sequence list and a gene encoding a plant-derived, asparagine residue-specific endoprotease having an amino sequence described in SEQ ID NO: 2 in the sequence listing. The enzymes enable production of asparagine residue-specific endoprotease in large amounts.
摘要翻译： 公开了编码具有序列表中SEQ ID NO：1所示的氨基序列的植物来源的天冬酰胺残基特异性内切蛋白酶的cDNA，以及编码具有氨基酸序列的植物来源的天冬酰胺残基特异性内切蛋白酶的基因 序列表中的SEQ ID NO：2。 这些酶能够大量生产天冬酰胺残基特异性内切蛋白酶。
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3.发明授权Method for preparing template DNA from processed vegetable food, which is feasible for amplification of DNA region by PCR method 失效从加工蔬菜食品中制备模板DNA的方法，可通过PCR方法扩增DNA区域公开(公告)号：US06492114B2
公开(公告)日：2002-12-10
申请号：US09494438
申请日：2000-01-31
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12N15/1003 , Y10T436/143333
摘要： Disclosed is a method for preparing a template DNA from a processed vegetable food, comprising the steps of: extracting a crude DNA fraction from a processed vegetable food subjected to at least one of a high temperature treatment, a high temperature grinding treatment, a high pressure treatment and a fermentation treatment, and optionally subjected to defatting; and performing a DNA fractionation treatment of the crude DNA fraction by a polyethylene glycol precipitation method and/or a polyacrylamide gel electrophoresis method. Also disclosed is a method for detecting a gene of a plant in a processed vegetable food derived from the plant by a PCR method in which such a template DNA is used. The processed vegetable food may be an oil source seed.
摘要翻译： 本发明公开了一种从加工蔬菜食品中制备模板DNA的方法，包括以下步骤：从经过高温处理，高温研磨处理，高压 处理和发酵处理，任选地进行脱脂; 并通过聚乙二醇沉淀法和/或聚丙烯酰胺凝胶电泳法对粗制DNA部分进行DNA分级处理。 还公开了通过使用这样的模板DNA的PCR法检测来自植物的加工植物性食品中的植物的基因的方法。 经处理的蔬菜食品可以是油源种子。
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4.发明授权CDNA encoding plant-derived epoxide hydrolase, gene encoding same and transformant 失效CDNA编码植物来源的环氧化物水解酶，基因编码相同和转化体公开(公告)号：US06372469B1
公开(公告)日：2002-04-16
申请号：US09412600
申请日：1999-10-06
IPC分类号： C12N912
CPC分类号： C12N9/14
摘要： Isolation of a cDNA encoding a plant-derived epoxide hydrolase and an expression system of the enzyme in Escherichia coli are established, so as to permit the mass-scale production of the plant-derived epoxide hydrolase. The invention provides a cDNA encoding the plant-derived epoxide hydrolase having the amino acid sequence of SQ ID No. 1 in the Sequence Listing, a gene encoding the plant-derived epoxide hydrolase having the amino acid sequence of SQ ID No. 2 in the Sequence Listing, a plasmid vector carrying the cDNA and the transformant (FERM BP-6624) retaining the plasmid vector.
摘要翻译： 建立了编码植物来源的环氧化物水解酶的cDNA和大肠杆菌中的酶的表达系统的分离，从而允许大规模生产植物来源的环氧化物水解酶。 本发明提供了编码植物来源的环氧化物水解酶的cDNA，该水解酶具有序列表中的SQID No.1序列的氨基酸序列，该基因编码植物来源的环氧化物水解酶，该氨基酸序列具有SEQ ID No.2的氨基酸序列 序列表，携带有保留质粒载体的cDNA和转化体（FERM BP-6624）的质粒载体。
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5.发明授权Quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease 失效用于植物来源的天冬酰胺残基特异性内切蛋白酶活性的快速测定方法公开(公告)号：US06251623B1
公开(公告)日：2001-06-26
申请号：US09410025
申请日：1999-10-01
IPC分类号： C12Q137
CPC分类号： C12Q1/37 , Y10S435/814
摘要： A quick assay method for the activity of a plant-derived, asparagine residue-specific endoprotease is disclosed which comprises measuring fluorescence generated by a fluorescence quenching substrate split by an asparagine residue-specific endoprotease. The fluorescence quenching substrate comprises an oligopeptide having in its amino acid sequence at least one asparagine residue, whose C-terminal side is other than an isoleucine residue, a leucine residue, or a valine residue, wherein the oligopeptide has a 7-methoxycoumarin-4-yl-acetyl group arranged on its N-terminal side and a 2,4-dinitrophenyl group arranged on its C-terminal side.
摘要翻译： 公开了一种用于植物衍生的天冬酰胺残基特异性内切蛋白酶活性的快速测定方法，其包括测量由天冬酰胺残基特异性内切蛋白酶分裂的荧光猝灭底物产生的荧光。 荧光猝灭底物包含在其氨基酸序列中具有至少一个天冬酰胺残基的寡肽，其C末端侧不是异亮氨酸残基，亮氨酸残基或缬氨酸残基，其中寡肽具有7-甲氧基香豆素-4 - 酰基乙酰基和其C-末端侧排列的2,4-二硝基苯基。
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