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公开(公告)号：US07575892B2
公开(公告)日：2009-08-18
申请号：US10590661
申请日：2005-02-25
CPC分类号： C12N9/0004 , C12N9/0083 , C12P33/06
摘要： Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
摘要翻译： 通过其前体β-二氨基吡啶的两步羟基化反应生物合成大豆皂甙B. 然而，该反应中涉及的羟化酶的基因尚未被揭示。 因此，不可能在羟化酶上应用遗传工程技术。 本发明人发现，与大豆来源的细胞色素P-450基因CYP93E1相对应的序列编码进行炔丙醇型三萜烯的24位的羟基化的酶蛋白，并且还提供了使用所述基因使用的方法 的遗传工程技术。
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公开(公告)号：US20080003639A1
公开(公告)日：2008-01-03
申请号：US10590661
申请日：2005-02-25
CPC分类号： C12N9/0004 , C12N9/0083 , C12P33/06
摘要： Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
摘要翻译： 通过其前体β-二氨基吡啶的两步羟基化反应生物合成大豆皂甙B. 然而，该反应中涉及的羟化酶的基因尚未被揭示。 因此，不可能在羟化酶上应用遗传工程技术。 本发明人发现，与大豆来源的细胞色素P-450基因CYP93E1相对应的序列编码进行炔丙醇型三萜烯的24位的羟基化的酶蛋白，并且还提供了使用所述基因使用的方法 的遗传工程技术。
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