公开(公告)号：US5384024A

公开(公告)日：1995-01-24

申请号：US850764

申请日：1992-03-13

申请人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

发明人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

IPC分类号： G01N27/447 ,  B01D61/46 ,  B01D57/02 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An instrument for capillary electrophoresis has a vertically translatable carrier holding one end of the capillary and an electrode connected to a power supply, and a rotary carousel for presenting containers of sample and buffer solutions to a load position where the end of the capillary and the electrode may be inserted by operating the carrier. The other end of the capillary and a second electrode connected to the power supply are immersed in an outlet buffer reservoir. The instrument has a vacuum supply for providing a relative vacuum over the solution in the outlet buffer reservoir, so simple material may be injected into the capillary either by electromigration or by differential pressure. After injection, the first end of the capillary and the electrode may be immersed in buffer to accomplish electrophoresis. By manipulating the carousel and carrier, multiple samples may be electrophoresed in series. In a preferred embodiment the instrument includes a flexible duck-billed septum for closing containers while permitting easy access for the electrode and the capillary tube. Also in a preferred embodiment the instrument is controlled by a microprocessor-based controller.

摘要翻译： 用于毛细管电泳的仪器具有保持毛细管的一端和连接到电源的电极的可垂直平移的载体，以及用于将样品和缓冲溶液的容器呈现到毛细管和电极的端部的负载位置的旋转转盘 可以通过操作载体来插入。 毛细管的另一端和连接到电源的第二电极浸入出口缓冲储存器中。 该仪器具有用于在出口缓冲储存器中的溶液上提供相对真空的真空源，因此可以通过电迁移或通过差压将简单的材料注入毛细管。 注射后，将毛细管和电极的第一端浸入缓冲液中以完成电泳。 通过操纵转盘和载体，可以将多个样品串联进行电泳。 在优选实施例中，该仪器包括用于封闭容器的柔性鸭嘴孔隔膜，同时允许容易地接近电极和毛细管。 同样在优选实施例中，仪器由基于微处理器的控制器控制。