公开(公告)号：US20140093864A1

公开(公告)日：2014-04-03

申请号：US13632677

申请日：2012-10-01

申请人： Csaba Pribenszky ,  Miklós Molnár ,  András Horváth

发明人： Csaba Pribenszky ,  Miklós Molnár ,  András Horváth

IPC分类号： C12N5/071 ,  C12N1/04 ,  C12N1/20 ,  C12N5/076 ,  C12N5/075

CPC分类号： C12N1/04 ,  A01N1/0289

摘要： The present invention relates to a method for improving viability and/or stress tolerance of viable biological material and using the said material comprising applying hydrostatic pressure to said biological material; keeping the said viable biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and using the said material for any desired purpose in accordance with any useful protocol. The usage of the said biological material incorporates any techniques, protocols that are applicable in the field of assisted reproductive techniques, biotechnical and/or biotechnological manipulations.

摘要翻译： 本发明涉及一种用于改善活的生物材料的活力和/或应力耐受性的方法，并且使用所述材料包括向所述生物材料施加静水压力; 将所述可行的生物材料保持在静水压力下一段预定时间段; 释放静水压力; 并根据任何有用的方案将所述材料用于任何所需目的。 所述生物材料的使用包括适用于辅助生殖技术，生物技术和/或生物技术操作领域的任何技术方案。

公开(公告)号：US08415093B2

公开(公告)日：2013-04-09

申请号：US13016512

申请日：2011-01-28

申请人： Csaba Pribenszky ,  Miklós Molnár

发明人： Csaba Pribenszky ,  Miklós Molnár

IPC分类号： A01N1/00

CPC分类号： A01N1/0289 ,  A01N1/02

摘要： The present invention relates to a method for improving post-thaw survival of cryopreserved biological material comprising applying hydrostatic pressure to said biological material; keeping the said biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and freezing the said biological material using any protocol applicable thereto.

摘要翻译： 本发明涉及一种用于改善冷冻保存的生物材料的解冻后存活的方法，包括对所述生物材料施加静水压力; 将所述生物材料保持在静水压力达预定时间段; 释放静水压力; 并使用适用于其的任何方案冷冻所述生物材料。

公开(公告)号：US20110189648A1

公开(公告)日：2011-08-04

申请号：US13016512

申请日：2011-01-28

申请人： Csaba Pribenszky ,  Miklós Molnár

发明人： Csaba Pribenszky ,  Miklós Molnár

IPC分类号： A01N1/02 ,  C12N5/071 ,  C12N5/075 ,  C12N5/073

CPC分类号： A01N1/0289 ,  A01N1/02

摘要： The present invention relates to a method for improving post-thaw survival of cryopreserved biological material comprising applying hydrostatic pressure to said biological material; keeping the said biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and freezing the said biological material using any protocol applicable thereto.

摘要翻译： 本发明涉及一种用于改善冷冻保存的生物材料的解冻后存活的方法，包括对所述生物材料施加静水压力; 将所述生物材料保持在静水压力达预定时间段; 释放静水压力; 并使用适用于其的任何方案冷冻所述生物材料。

公开(公告)号：US20140329222A1

公开(公告)日：2014-11-06

申请号：US14332910

申请日：2014-07-16

申请人： Csaba PRIBENSZKY ,  Miklós MOLNÁR ,  András HORVÁTH

发明人： Csaba PRIBENSZKY ,  Miklós MOLNÁR ,  András HORVÁTH

IPC分类号： C12N5/076 ,  C12N5/073 ,  C12N5/075 ,  A61D19/04 ,  A61B17/43 ,  A61B17/435 ,  A61D19/02 ,  C12N1/36 ,  C12N5/0735

CPC分类号： A61D19/04 ,  A01N1/0289 ,  A61B17/43 ,  A61B17/435 ,  A61D19/02 ,  C12N1/04

摘要： The present invention relates to a method for improving viability and/or stress tolerance of viable biological material and using the said material comprising applying hydrostatic pressure to said biological material; keeping the said viable biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and using the said material for any desired purpose in accordance with any useful protocol. The usage of the said biological material incorporates any techniques, protocols that are applicable in the field of assisted reproductive techniques, biotechnical and/or biotechnological manipulations.

公开(公告)号：US20130215252A1

公开(公告)日：2013-08-22

申请号：US13829924

申请日：2013-03-14

申请人： Csaba Pribenszky ,  Miklós Molnár

发明人： Csaba Pribenszky ,  Miklós Molnár

IPC分类号： H04N7/18

CPC分类号： H04N7/18 ,  C12M21/06 ,  C12M41/14 ,  C12M41/36 ,  C12M41/46 ,  G02B21/361 ,  G02B21/362 ,  G02B21/365

摘要： A microscope unit positioned in a culturing space for imaging cells or tissues also located therein. The microscope unit has a frame, an object holder provided on the frame and allowing the cells or tissues to be held substantially immobile during a culturing period, an imaging device arranged on the frame for the optical imaging of the cells or tissues held on the object holder and an image capturing device for capturing an image projected by the imaging device. A connection leading out of the culturing space provides electrical power to the microscope unit and is adapted to be connected to a control unit and, in turn, a processor, both located outside of the culturing space. The invention also relates to a method for monitoring cells or tissues located in the culturing space and transmitting the image thereof.

摘要翻译： 位于培养空间中用于成像也位于其中的细胞或组织的显微镜单元。 显微镜单元具有框架，设置在框架上的物体保持器，并且允许细胞或组织在培养期间基本上不能移动;成像装置，布置在框架上，用于对保持在物体上的细胞或组织进行光学成像 保持器和用于捕获由成像装置投影的图像的图像捕获装置。 从培养空间导出的连接为显微镜单元提供电力，并且适于连接到控制单元，并且还连接到位于培养空间外部的处理器。 本发明还涉及一种用于监测位于培养空间中并传播其图像的细胞或组织的方法。

公开(公告)号：US08278031B2

公开(公告)日：2012-10-02

申请号：US12094582

申请日：2006-11-21

申请人： Csaba Pribenszky ,  Miklós Molnár ,  András Horváth

发明人： Csaba Pribenszky ,  Miklós Molnár ,  András Horváth

IPC分类号： A01N1/00 ,  A61B17/43

CPC分类号： A01N1/02 ,  A01N1/0289

摘要： The present invention relates to a method for improving viability and/or stress tolerance of viable biological material and using the said material comprising applying hydrostatic pressure to said biological material; keeping the said viable biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and using the said material for any desired purpose in accordance with any useful protocol. The usage of the said biological material incorporates any techniques, protocols that are applicable in the field of assisted reproductive techniques, biotechnical and/or biotechnological manipulations.

摘要翻译： 本发明涉及一种用于改善活的生物材料的活力和/或应力耐受性的方法，并且使用所述材料包括向所述生物材料施加静水压力; 将所述可行的生物材料保持在静水压力下一段预定时间段; 释放静水压力; 并根据任何有用的方案将所述材料用于任何所需目的。 所述生物材料的使用包括适用于辅助生殖技术，生物技术和/或生物技术操作领域的任何技术方案。

公开(公告)号：US07879539B2

公开(公告)日：2011-02-01

申请号：US10571236

申请日：2004-09-08

申请人： Csaba Pribenszky ,  Miklós Molnár

发明人： Csaba Pribenszky ,  Miklós Molnár

IPC分类号： A01N1/00

CPC分类号： A01N1/02 ,  A01N1/0289

摘要： The present invention relates to a method for improving post-thaw survival of cryopreserved biological material comprising applying hydrostatic pressure to said biological material; keeping the said biological material at the hydrostatic pressure for a predetermined time period; releasing the hydrostatic pressure; and freezing the said biological material using any protocol applicable thereto. The invention also relates to the use of a pressurizing device for the pretreatment of a biological material that is to be cryopreserved, as well as to a pressurizing device for the pretreatment of a biological material that is to be cryopreserved, said device comprising a pressure chamber for receiving biological material, means to produce said pressure, and means to maintain said pressure in said chamber.

摘要翻译： 本发明涉及一种用于改善冷冻保存的生物材料的解冻后存活的方法，包括对所述生物材料施加静水压力; 将所述生物材料保持在静水压力达预定时间段; 释放静水压力; 并使用适用于其的任何方案冷冻所述生物材料。 本发明还涉及一种加压装置用于对待冷冻保存的生物材料进行预处理的加压装置以及用于预冷冻待生物材料的加压装置，所述装置包括压力室 用于接收生物材料，产生所述压力的装置，以及在所述室中保持所述压力的装置。

公开(公告)号：US20120140056A1

公开(公告)日：2012-06-07

申请号：US13382619

申请日：2010-07-09

申请人： Csaba Pribenszky ,  Miklós Molnár

发明人： Csaba Pribenszky ,  Miklós Molnár

IPC分类号： H04N7/18

CPC分类号： H04N7/18 ,  C12M21/06 ,  C12M41/14 ,  C12M41/36 ,  C12M41/46 ,  G02B21/361 ,  G02B21/362 ,  G02B21/365

摘要： The invention relates to a sample imaging system (1) for transmitting an image of cells or tissues located in a culturing space (6) to data processing means (7), including a microscope unit (2) intended to be used in the culturing space (6), the microscope unit (2) comprising a frame, an object holder provided on the frame and allowing the cells or tissues to be held substantially immobile during a culturing period, an imaging means arranged on the frame for the optical imaging of the cells or tissues held on the object holder and an image capturing means capturing an image projected by the imaging means. Furthermore, the microscope unit (2) has a connecting means (4) being able to be led out of the culturing space (6) and providing electrical power supply to the microscope unit (2) and transmitting the captured image and the connecting means (4), which connecting means (4) is adapted to be connected to a control unit (3) intended to be used outside of the culturing space (6) and transmitting the captured image to the data processing means (7) and the control unit (3) comprises means adapted to suspend the electrical power supply of the microscope unit (2) with the exception of a period for capturing the image of the cells or tissues and transmitting the captured image to the control unit (3) via the connecting means (4). The invention also relates to a method for transmitting the image of the cells or tissues located in the culturing space (6) to the data processing means (7).

摘要翻译： 本发明涉及一种用于将位于培养空间（6）中的细胞或组织的图像传送到数据处理装置（7）的样品成像系统（1），包括用于培养空间的显微镜单元（2） （6），所述显微镜单元（2）包括框架，设置在所述框架上的物体保持器，并且允许所述细胞或组织在培养期间基本上不能移动;成像装置，布置在所述框架上用于所述 保持在物体保持器上的细胞或组织和捕获由成像装置投影的图像的图像捕获装置。 此外，显微镜单元（2）具有能够从培养空间（6）引出并且向显微镜单元（2）提供电力并将捕获的图像和连接装置（ 4），连接装置（4）适于连接到旨在在培养空间（6）外部使用的控制单元（3），并将捕获的图像发送到数据处理装置（7）和控制单元 （3）包括用于暂停显微镜单元（2）的电源的装置，除了用于捕获小区或组织的图像的周期之外，并且经由连接装置将捕获的图像发送到控制单元（3） （4）。 本发明还涉及一种将位于培养空间（6）中的细胞或组织的图像传送到数据处理装置（7）的方法。