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公开(公告)号：US5914109A
公开(公告)日：1999-06-22
申请号：US345321
申请日：1994-11-21
IPC分类号： A61P31/18 , C07K14/16 , C07K16/10 , C12N5/26 , A61K39/395
CPC分类号： C07K14/005 , C07K16/1063 , C12N5/166 , C12N2740/16122
摘要： Heterohybridomas producing human monoclonal antibodies against a neutralizing epitope of HIV-1 are prepared by transforming peripheral blood lymphocytes by Epstein-Barr virus, selecting cells producing an antibody to a synthetic peptide representing part of the neutralizing epitope, and fusing them with a heteromyeloma cell. A method for producing a human monoclonal antibody specific for a neutralizing HIV-1 epitope from the heterohybridoma is disclosed. Also provided are human neutralizing monoclonal antibodies specific for particular peptide epitopes of the V3 loop of the gp120 glycoprotein of HIV, in particular a broadly reactive antibody specific for diverse HIV-1 strains, preferably recognizing an epitope comprising an amino acid sequence GPXR or GRAF.
摘要翻译： 通过用Epstein-Barr病毒转化外周血淋巴细胞，选择产生代表部分中和表位的合成肽的抗体的细胞，并将它们与异源骨髓瘤细胞融合，制备产生HIV-1中和表位的人单克隆抗体的杂交杂交瘤。 公开了一种从异源杂交瘤产生中和HIV-1表位特异性的人单克隆抗体的方法。 还提供了对于HIV的gp120糖蛋白的V3环的特定肽表位特异的人中和单克隆抗体，特别是针对不同HIV-1毒株特异的广泛反应性抗体，优选识别包含氨基酸序列GPXR或GRAF的表位。
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2.发明申请IMMUNOGEN PRESENTING HIV GP120 V3 LOOP IN A CONFORMATION THAT INDUCES BROADLY NEUTRALIZING ANTIBODIES 有权免疫细胞提供HIV GP120 V3环路，诱导大量中和抗体公开(公告)号：US20090098144A1
公开(公告)日：2009-04-16
申请号：US12195318
申请日：2008-08-20
申请人： Susan ZOLLA-PAZNER , Miroslaw K. Gorny , Timothy J. Cardozo , Xiang-peng Kong , Ruben Abagyan , Maxim Totrov , Shan Lu , Abraham Pinter
发明人： Susan ZOLLA-PAZNER , Miroslaw K. Gorny , Timothy J. Cardozo , Xiang-peng Kong , Ruben Abagyan , Maxim Totrov , Shan Lu , Abraham Pinter
CPC分类号： C07K14/005 , A61K38/03 , A61K38/16 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/545 , A61K2039/55555 , C07K2319/35 , C07K2319/55 , C12N2740/16122 , C12N2740/16134 , Y02A50/472
摘要： Insertion of HIV-1 V3 loop peptides from the viral glycoprotein gp120 into selected, immunogenic scaffold proteins results in a recombinant polypeptide that is a potent V3 immunogen. V3 immunogens include natural and consensus V3 sequences and cyclic and reverse peptides. Preferred scaffold proteins are Cholera Toxin subunit B and homologues thereof including closely related E. coli enterotoxins. Such immunogenic polypeptides induce broadly reactive anti-gp120 antibodies specific for V3 epitopes that can neutralize heterologous HIV-1 subtypes and strains. These polypeptide, methods for preparing them, and methods for inducing anti-gp120 (V3-specific) antibody) responses using them are disclosed.
摘要翻译： 将HIV-1 V3环肽从病毒糖蛋白gp120插入到选定的免疫原性支架蛋白中导致重组多肽，其是有效的V3免疫原。 V3免疫原包括天然和共有的V3序列和环状和反向肽。 优选的支架蛋白是霍乱毒素亚基B及其同系物，包括密切相关的大肠杆菌肠毒素。 这样的免疫原性多肽诱导可以中和异源HIV-1亚型和菌株的V3表位特异性的广泛反应性抗gp120抗体。 公开了这些多肽，其制备方法和诱导抗gp120（V3特异性）抗体的方法）。
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3.发明授权Immunogen comprising the HIV GP120 V3 loop in a conformation that induces broadly neutralizing antibodies 有权包含诱导广泛中和抗体的构象的HIV GP120 V3环的免疫原公开(公告)号：US08961987B2
公开(公告)日：2015-02-24
申请号：US12195318
申请日：2008-08-20
申请人： Susan Zolla-Pazner , Miroslaw K. Gorny , Timothy J. Cardozo , Xiang-peng Kong , Ruben Abagyan , Maxim Totrov , Shan Lu , Abraham Pinter
发明人： Susan Zolla-Pazner , Miroslaw K. Gorny , Timothy J. Cardozo , Xiang-peng Kong , Ruben Abagyan , Maxim Totrov , Shan Lu , Abraham Pinter
CPC分类号： C07K14/005 , A61K38/03 , A61K38/16 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/545 , A61K2039/55555 , C07K2319/35 , C07K2319/55 , C12N2740/16122 , C12N2740/16134 , Y02A50/472
摘要： Insertion of HIV-1 V3 loop peptides from the viral glycoprotein gp120 into selected, immunogenic scaffold proteins results in a recombinant polypeptide that is a potent V3 immunogen. V3 immunogens include natural and consensus V3 sequences and cyclic and reverse peptides. Preferred scaffold proteins are Cholera Toxin subunit B and homologues thereof including closely related E. coli enterotoxins. Such immunogenic polypeptides induce broadly reactive anti-gp120 antibodies specific for V3 epitopes that can neutralize heterologous HIV-1 subtypes and strains. These polypeptide, methods for preparing them, and methods for inducing anti-gp120 (V3-specific) antibody) responses using them are disclosed.
摘要翻译： 将HIV-1 V3环肽从病毒糖蛋白gp120插入到选定的免疫原性支架蛋白中导致重组多肽，其是有效的V3免疫原。 V3免疫原包括天然和共有的V3序列和环状和反向肽。 优选的支架蛋白是霍乱毒素亚基B及其同系物，包括密切相关的大肠杆菌肠毒素。 这样的免疫原性多肽诱导可以中和异源HIV-1亚型和菌株的V3表位特异性的广泛反应性抗gp120抗体。 公开了这些多肽，其制备方法和诱导抗gp120（V3特异性）抗体的方法）。
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4.发明授权Human monoclonal antibodies to the CD4-binding domain of HIV, uses thereof and synergistic neutralization of HIV 失效针对HIV的CD4结合域的人单克隆抗体，其使用和HIV的协同中和公开(公告)号：US06241986B1
公开(公告)日：2001-06-05
申请号：US08215395
申请日：1994-03-21
IPC分类号： A61K3942
CPC分类号： C07K16/1063 , A61K38/00 , C07K2317/34
摘要： Human monoclonal antibodies specific for the CD4-binding domain of HIV gp120 are useful in the neutralization of HIV and in the prevention of HIV infection and the treatment of a subject infected with HIV. Such antibodies and heterohybridomas producing them are disclosed. Synergistic mixtures of at least two human monoclonal antibodies specific for two different epitopes of gp120 are used to neutralize HIV, to prevent HIV infection and to treat a subject infected with HIV. In this synergistic mixture, one antibody has broad HIV group specificity, and is preferably specific for the CD4-binding domain. The other antibody is preferably specific for the V3 loop and has a range of neutralizing activity such that it neutralizes virus of the MN strain, MN-like families, and widely divergent HIV-1 isolates which are members of various V3 lop classes.
摘要翻译： 对HIV gp120的CD4结合结构域特异的人单克隆抗体可用于HIV的中和和HIV感染的预防以及感染HIV的受试者的治疗。 公开了这样的抗体和产生它们的异源杂交瘤。 使用对gp120的两个不同表位特异的至少两种人单克隆抗体的协同混合物来中和HIV，以防止HIV感染并治疗感染HIV的受试者。 在该协同混合物中，一种抗体具有广泛的HIV组特异性，并且优选对CD4结合结构域是特异性的。 其它抗体优选对V3环具有特异性，并且具有一定范围的中和活性，使得其中和MN菌株，MN样家族的病毒和广泛分散的HIV-1分离物，其是各种V3 lop等级的成员。
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公开(公告)号：US5731189A
公开(公告)日：1998-03-24
申请号：US364007
申请日：1994-12-27
CPC分类号： C07K16/1063 , C07K16/1054 , C07K2317/732
摘要： Disclosed herein are eleven human lymphoblastoid cell lines producing monoclonal antibodies directed against human immunodeficiency virus (HIV) proteins gp41 and p24. Also disclosed are methods for treating HIV-infected individuals using the human monoclonal antibodies and pharmaceutical formulations comprising effective amounts of the human monoclonal antibodies.
摘要翻译： 本文公开了产生针对人免疫缺陷病毒（HIV）蛋白gp41和p24的单克隆抗体的11种人类淋巴母细胞样细胞系。 还公开了使用人单克隆抗体治疗HIV感染个体的方法和包含有效量的人单克隆抗体的药物制剂。
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