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公开(公告)号:US08206969B2
公开(公告)日:2012-06-26
申请号:US12809118
申请日:2008-12-18
申请人: Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
发明人: Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
IPC分类号: C12N9/84
摘要: A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.
摘要翻译: 具有丙烯酸酯化活性的蛋白质通过引入至少一种下列氨基酸取代:具有SEQ ID NO:1或NO:2的多肽序列:L278A,L278V,W104F,T42A,S47A。
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公开(公告)号:US20100273223A1
公开(公告)日:2010-10-28
申请号:US12809118
申请日:2008-12-18
申请人: Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
发明人: Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
摘要: A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.
摘要翻译: 具有丙烯酸酯化活性的蛋白质通过引入至少一种下列氨基酸取代:具有SEQ ID NO:1或NO:2的多肽序列:L278A,L278V,W104F,T42A,S47A。
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公开(公告)号:US20100151502A1
公开(公告)日:2010-06-17
申请号:US12295889
申请日:2007-04-05
申请人: Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
发明人: Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
IPC分类号: G01N33/554 , C12N9/20 , C07H21/00 , C12N15/74 , C12N1/21
CPC分类号: C12N9/20 , C12Q1/44 , C12Q1/61 , G01N2333/918
摘要: The present invention relates to nucleic acids that code for triacylglycerol lipases, vectors comprising said nucleic acids, host cells comprising said nucleic acids or vectors, methods for the expression of triacylglycerol lipases in prokaryotes, methods for the detection and for the production of triacylglycerol lipases, triacylglycerol lipases obtainable thereby, and the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for said methods.
摘要翻译: 本发明涉及编码三酰基甘油脂肪酶的核酸,包含所述核酸的载体,包含所述核酸或载体的宿主细胞,在原核生物中表达三酰甘油脂肪酶的方法,用于检测和产生三酰甘油脂肪酶的方法, 由此获得的三酰基甘油脂酶,以及用于所述方法的用于酰基甘油脂肪酶编码核酸,载体和重组宿主细胞的用途。
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