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公开(公告)号：US4450238A
公开(公告)日：1984-05-22
申请号：US439378
申请日：1982-11-05
CPC分类号： C12N9/2465 , Y10S435/942
摘要： The invention relates to a process for the production of the alpha-galactosidase enzyme by culturing yeasts of the Saccharomyces cerevisiae genus in a temperature range from 20.degree. C. to 40.degree. C.The invention also relates to a process for enzymic hydrolysis of raffinose by alpha-galactosidase from Saccharomyces cerevisiae. Such hydrolysis may take place with the yeast cells being present or also in the presence of enzymic extracts, both as such and enriched. An important advantage of the invention is the high alpha-galactosidasic activity of the selected microorganisms, together with the absence of any invertasic activity.
摘要翻译： 本发明涉及通过在20℃至40℃的温度范围内培养酿酒酵母的酵母来生产α-半乳糖苷酶的方法。本发明还涉及一种通过以下方法酶棉酶的水解方法： 来自酿酒酵母的α-半乳糖苷酶。 这种水解可以在酵母细胞存在的情况下进行，也可以在酶提取物的存在下进行，既可以这样又富集。 本发明的一个重要优点是选择的微生物的高α-半乳糖苷酶活性高，以及不存在任何非转化活性。
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2.发明授权Process for producing the enzyme cholesterol esterase and for hydrolyzing cholesterol esters of fatty acids by using the enzyme itself 失效通过使用酶本身，生产酶胆固醇酯酶和用于水解脂肪酸胆固醇酯的方法
公开(公告)号：US4387163A
公开(公告)日：1983-06-07
申请号：US282744
申请日：1981-07-13
CPC分类号： C12R1/025 , C12N9/18 , Y10S435/824
摘要： The enzyme cholesterol esterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exocellular and therefore does not require extraction from the cells.In addition, the micro-organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium before inoculating the strain.
摘要翻译： 酶胆固醇酯酶是通过培养从蔬菜碎片分离并通过符号NRRL B-12115鉴定的无精细型无色杆菌菌株产生的。 在所述培养条件下，酶是细胞外的，因此不需要从细胞中提取。 此外，微生物的复制时间小于霉菌和放线菌的重复时间。 脂肪酸的胆固醇酯可以在接种菌株之前将其加入到培养基中来水解。
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公开(公告)号：US4376167A
公开(公告)日：1983-03-08
申请号：US213657
申请日：1980-12-05
CPC分类号： C12N9/2465 , Y10S435/942
摘要： The invention relates to a process for the production of the alpha-galactoxidase enzyme by culturing yeasts of the Saccharomyces cerevisiae genus in a temperature range from 20.degree. C. to 40.degree. C.The invention also relates to a process for enzymic hydrolysis of raffinose by alpha-galactoxidase from Saccharomyces cerevisiae. Such hydrolysis may take place with the yeast cells being present or also in the presence of enzymic extracts, both as such and enriched. An important advantage of the invention is the high alpha-galactoxidasic activity of the selected microorganisms, together with the absence of any invertasic activity.
摘要翻译： 本发明涉及通过在20℃至40℃的温度范围内培养酿酒酵母的酵母来生产α-半乳糖氧化酶的方法。本发明还涉及一种通过以下步骤将棉子糖酶水解的方法： 来自酿酒酵母的α-半乳糖氧化酶。 这种水解可以在酵母细胞存在的情况下进行，也可以在酶提取物的存在下进行，既可以这样又富集。 本发明的一个重要优点是选择的微生物的高α-半乳糖氧化活性高，以及不存在任何非转化活性。
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4.发明授权Production of fructose and fructose-base syrups and means for carrying out such production 失效生产果糖和果糖基糖浆以及进行这种生产的方法
公开(公告)号：US4291123A
公开(公告)日：1981-09-22
申请号：US913536
申请日：1978-06-08
申请人： Ludwig Degen , Paolo Branduzzi , Roberto Olivieri , Nadia Cimini
发明人： Ludwig Degen , Paolo Branduzzi , Roberto Olivieri , Nadia Cimini
CPC分类号： C13K11/00 , C12N9/92 , Y10S435/886
摘要： A method is disclosed for the production of fructose and syrups containing fructose and glucose, comprising the step of contacting a solution of glucose with a micro-organism of the genus Streptomyces sp. and more particularly of the strains NRRL 11.120 and NRRL 11.121, as designated by the Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Ill.
摘要翻译： 公开了用于生产含有果糖和葡萄糖的果糖和糖浆的方法，包括使葡萄糖溶液与链霉菌属（Streptomyces sp。）的微生物接触的步骤。 更具体地是由北方地区研究中心指定的NRRL 11.120和NRRL 11.121菌株，美国农业部Peoria，Ill。
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