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    METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI
    2.
    发明申请
    METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI 有权
    使用重组蛋白酶生产柠檬酸的方法

    公开(公告)号：US20120295322A1

    公开(公告)日：2012-11-22

    申请号：US13536455

    申请日：2012-06-28

    申请人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    发明人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    IPC分类号： C12P5/02

    CPC分类号： C12P23/00 ,  C12N9/0004 ,  C12N9/0083 ,  C12N9/1085 ,  C12N15/52

    摘要： A method of producing lycopene, with high productivity by means of a recombinant bacterial strain includes preparing the recombinant vector containing genes encoding proteins, which are required for lycopene biosynthesis. The genes involved in lycopene biosynthesis are crtE, crtB and crtI, and at least one of the said three genes (crtE, crtB and crtI) is selected from the group consisting of crtE with the SEQ ID NO:1, crtB with the SEQ ID NO:3 and crtI with the SEQ ID NO:5, of the Sequence List. The said recombinant vector is transformed into Escherichia coli (hereafter E. coli). The E. coli transformant is cultured to recover lycopene from the culture medium.

    摘要翻译： 通过重组细菌菌株以高生产率生产番茄红素的方法包括制备含有编码蛋白质的基因的重组载体，其是番茄红素生物合成所需的。 涉及番茄红素生物合成的基因是crtE，crtB和crtI，并且所述三种基因（crtE，crtB和crtI）中的至少一种选自由SEQ ID NO：1的crtE，具有SEQ ID NO：1的crtB组成的组 和序列表中SEQ ID NO：5的crtI。 将所述重组载体转化到大肠杆菌（以下称为大肠杆菌）中。 培养大肠杆菌转化体以从培养基中回收番茄红素。

    GENE INVOLVED IN THE BIOSYNTHESES OF LYCOPENE, RECOMBINANT VECTOR COMPRISING THE GENE, AND TRANSFORMED MICROORGANISM WITH THE RECOMBINANT VECTOR
    3.
    发明申请
    GENE INVOLVED IN THE BIOSYNTHESES OF LYCOPENE, RECOMBINANT VECTOR COMPRISING THE GENE, AND TRANSFORMED MICROORGANISM WITH THE RECOMBINANT VECTOR 审中-公开
    涉及LYCOPENE的生物合成基因，包含该基因的重组载体和与重组载体的转化的微生物

    公开(公告)号：US20110124090A1

    公开(公告)日：2011-05-26

    申请号：US12594659

    申请日：2008-04-07

    申请人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    发明人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    IPC分类号： C12N1/21 ,  C07H21/04 ,  C12N15/70

    CPC分类号： C12N9/0004 ,  C12N9/1085 ,  C12P23/00

    摘要： There are provided genes involved in the biosynthesis of lycopene and having DNA sequences set forth in SEQ ID NO: 1, SEQ ID NO: 3 and SEQ ID NO: 5 encoding proteins required for the biosynthesis of lycopene, a recombinant vector comprising at least one of the genes, and a mi croorganism transformed with the recombinant vector and having a high content of lycopene. The lycopene is obtained at a yield of 15.3 mg/L and a content of 4.2 mg/gDCW when the recombined E. coli with the crt genes is cultivated, and the lycopene is also obtained with the maximum content of 5.4 mg/gDCW when a microorganism is transformed with the combination of the gene of the present invention and the known genes. Therefore, provided is the lycopene-producing strain having a more increased content of lycopene per dry cell weight than the known lycopene-producing strain with the genes. Accordingly, the genes may be useful to mass-produce lycopene in microorganisms, and also to mass-produce carotenoids.

    摘要翻译： 提供参与番茄红素生物合成的基因，并且具有SEQ ID NO：1，SEQ ID NO：3和SEQ ID NO：5所示的DNA序列，所述DNA序列编码番茄红素生物合成所需的蛋白质，重组载体包含至少一个 的基因，以及用重组载体转化并具有高含量番茄红素的微生物。 当培养具有crt基因的重组大肠杆菌时，以15.3mg / L的产率和4.2mg / gDCW的含量获得番茄红素，并且当获得最大含量为5.4mg / gDCW的番茄红素时 用本发明基因与已知基因的组合转化微生物。 因此，提供的番茄红素生产菌株的干细胞重量比具有该基因的已知番茄红素生产菌株的番茄红素含量更高。 因此，该基因可用于在微生物中大量生产番茄红素，也可用于大量生产类胡萝卜素。

    METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI
    4.
    发明申请
    METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI 有权
    使用重组蛋白酶生产柠檬酸的方法

    公开(公告)号：US20100227370A1

    公开(公告)日：2010-09-09

    申请号：US12598808

    申请日：2008-05-06

    申请人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    发明人： Nahm Ryune Cho ,  Min Soo Park ,  Dong Hyun Lee ,  Ho Seung Chung ,  Jong Keun Kim

    IPC分类号： C12P5/02

    CPC分类号： C12P23/00 ,  C12N9/0004 ,  C12N9/0083 ,  C12N9/1085 ,  C12N15/52

    摘要： A method of producing lycopene, with high productivity by means of a recombinant bacterial strain includes preparing the recombinant vector containing genes encoding proteins, which are required for lycopene biosynthesis. The genes involved in lycopene biosynthesis are crtE, crtB and crtI, and at least one of the said three genes (crtE, crtB and crtI) is selected from the group consisting of crtE with the nucleotide Sequence 1, crtB with the nucleotide Sequence 3 and crtI with the nucleotide Sequence 5, of the Sequence List. The said recombinant vector is transformed into Escherichia coli (hereafter E. coli). The E. coli transformant is cultured to recover lycopene from the culture medium.

    摘要翻译： 通过重组细菌菌株以高生产率生产番茄红素的方法包括制备含有编码蛋白质的基因的重组载体，其是番茄红素生物合成所需的。 涉及番茄红素生物合成的基因是crtE，crtB和crtI，所述三种基因（crtE，crtB和crtI）中的至少一种选自由核苷酸序列1的crtE，核苷酸序列3的crtB和 crtI与序列表的核苷酸序列5。 将所述重组载体转化到大肠杆菌（以下称为大肠杆菌）中。 培养大肠杆菌转化体以从培养基中回收番茄红素。

    Method for preparing (s)-indoline-2-carboxylic acid and (s)-indoline-2-carboxylic acid methyl ester using hydrolytic enzyme
    8.
    发明授权
    Method for preparing (s)-indoline-2-carboxylic acid and (s)-indoline-2-carboxylic acid methyl ester using hydrolytic enzyme 失效
    使用水解酶制备（S） - 二氢吲哚-2-羧酸和（S） - 二氢吲哚-2-羧酸甲酯的方法

    公开(公告)号：US07405070B2

    公开(公告)日：2008-07-29

    申请号：US10596068

    申请日：2004-11-03

    申请人： Nahm Ryune Cho ,  Jong Ho Lim ,  Jong Keun Kim

    发明人： Nahm Ryune Cho ,  Jong Ho Lim ,  Jong Keun Kim

    IPC分类号： C12P41/00 ,  C12P17/10 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16

    CPC分类号： C12P17/10 ,  C07D209/42 ,  C12P41/005

    摘要： Disclosed is a method for preparing (S)-indoline-2-carboxylic acid and (S)-indoline-2-carboxylic acid methyl ester using an inexpensive industrially available enzyme capable of assuring superior optical purity and yield. At this time, the hydrolytic enzyme is selected from the group consisting of Savinase, Alcalase, Novozym 243, Everlase, Esperase, Protease 7 and Acylase, whereby (S)-indoline-2-carboxylic acid and methyl ester thereof having an optical purity of at least 99% e.e. can be obtained through a simplified preparation process, thus generating economic benefits.

    摘要翻译： 公开了一种使用廉价的工业可得的酶制备（S） - 二氢吲哚-2-羧酸和（S） - 二氢吲哚-2-羧酸甲酯的方法，其能够确保优异的光学纯度和产率。 此时，水解酶选自Savinase，Alcalase，Novozym 243，Everlase，Esperase，Protease 7和Acylase，由此得到具有光学纯度的（S） - 二氢吲哚-2-羧酸及其甲酯 至少99％ee 可以通过简化的制备过程获得，从而产生经济效益。