公开(公告)号：US20190002924A1

公开(公告)日：2019-01-03

申请号：US15912385

申请日：2018-03-05

申请人： Defeng Xing ,  Najiaowa Yu ,  Wei Li ,  Zhen Li ,  Yang Yang

发明人： Defeng Xing ,  Najiaowa Yu ,  Wei Li ,  Zhen Li ,  Yang Yang

IPC分类号： C12P3/00 ,  C12N1/20 ,  C12R1/145 ,  C12R1/385 ,  C07H3/02 ,  C07H3/04 ,  C07C323/58

CPC分类号： C12P3/00 ,  C07C323/58 ,  C07H3/02 ,  C07H3/04 ,  C12N1/20 ,  C12R1/145 ,  C12R1/385

摘要： The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, E. harbinense YUAN-3, and a facultative anaerobic bacterium, P. aeruginosa PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth of E. harbinense YUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.

公开(公告)号：US10378028B2

公开(公告)日：2019-08-13

申请号：US15912385

申请日：2018-03-05

申请人： Defeng Xing ,  Najiaowa Yu ,  Wei Li ,  Zhen Li ,  Yang Yang

发明人： Defeng Xing ,  Najiaowa Yu ,  Wei Li ,  Zhen Li ,  Yang Yang

IPC分类号： C12N1/20 ,  C12P39/00 ,  C12P3/00 ,  C12R1/145 ,  C07C323/58 ,  C07H3/02 ,  C07H3/04 ,  C12R1/385

摘要： The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, E. harbinense YUAN-3, and a facultative anaerobic bacterium, P. aeruginosa PAO1. The two bacteria are co-cultured to produce hydrogen gas in a culture medium without any anaerobic treatment. Sucrose, lactose or glucose are used as the carbon source for the co-culture which can promote the growth of E. harbinense YUAN-3 and reduce substrate competition between two bacteria. L-cysteine is added to increase the hydrogen yield and the production rate. Using 15 g/L glucose and 5 mmol/L L-cysteine, the invented method achieved the hydrogen production yield of 1.11 mol-hydrogen/mol-glucose.