公开(公告)号：US20220002704A1

公开(公告)日：2022-01-06

申请号：US17479037

申请日：2021-09-20

Applicant: Naoki Satoh ,  Hidekazu Yaginuma ,  Tomoyuki Aratani ,  Momoko Shionoiri ,  Natsuko Iwashita ,  Tatsuya Sameshima ,  Takehiro Yamazaki

Inventor： Naoki Satoh ,  Hidekazu Yaginuma ,  Tomoyuki Aratani ,  Momoko Shionoiri ,  Natsuko Iwashita ,  Tatsuya Sameshima ,  Takehiro Yamazaki

IPC: C12N11/04

Abstract: This invention is to develop a production method for a cell culture carrier comprising a hydrogel having high shape retainability, and cell culture carrier production device, and to provide a structure composed of a cell culture carrier produced using the same.
The production method comprises a retention step of retaining on a support material a first solution containing a multiple branching polymer comprising polyethylene glycol as the backbone, and having at least one functional group which is one of a nucleophilic functional group and an electrophilic functional group at a side chain(s) and/or a terminal(s), and a gel formation step of forming one or more dot-shaped hydrogels in which a second solution containing a multiple branching polymer comprising polyethylene glycol as the backbone, and having at least one functional group which is the other one of a nucleophilic functional group and an electrophilic functional group at a side chain and/or a terminal, is discharged by a droplet discharge device to land in contact with the first solution retained on the support material.

公开(公告)号：US20190284524A1

公开(公告)日：2019-09-19

申请号：US16299873

申请日：2019-03-12

Applicant: Hidekazu YAGINUMA ,  Naoki SATOH ,  Tomoyuki ARATANI ,  Chihiro KUBO ,  Daisuke TAKAGI ,  Momoko SHIONOIRI ,  Natsuko IWASHITA

Inventor： Hidekazu YAGINUMA ,  Naoki SATOH ,  Tomoyuki ARATANI ,  Chihiro KUBO ,  Daisuke TAKAGI ,  Momoko SHIONOIRI ,  Natsuko IWASHITA

IPC: C12N5/00 ,  G16B40/00

Abstract: Provided is a cell tissue producing method including: a cell adhesive portion forming step of forming a cell adhesive portion formed of a cell adhesive material and having a predetermined shape at a predetermined position over a substrate having a cell non-adhesive surface; and a cell locating step of discharging a cell suspension containing at least cells and a cell drying inhibitor onto the cell adhesive portion in the form of a liquid droplet to locate the cells.

公开(公告)号：US20210154932A1

公开(公告)日：2021-05-27

申请号：US16950133

申请日：2020-11-17

Applicant: Tatsuya SAMESHIMA ,  Hidekazu YAGINUMA ,  Takahiko MATSUMOTO ,  Momoko SHIONOIRI ,  Natsuko IWASHITA ,  Chihiro KUBO ,  Naoki SATOH ,  Takehiro YAMAZAKI

Inventor： Tatsuya SAMESHIMA ,  Hidekazu YAGINUMA ,  Takahiko MATSUMOTO ,  Momoko SHIONOIRI ,  Natsuko IWASHITA ,  Chihiro KUBO ,  Naoki SATOH ,  Takehiro YAMAZAKI

IPC: B29C64/209 ,  B33Y30/00 ,  B33Y70/00

Abstract: A liquid discharge head including: a film member including a discharge hole configured to discharge liquid; and a displacement member configured to displace a position of the film member to discharge the liquid from the discharge hole, wherein an edge of the film member forming the discharge hole has a curve in a cross section of the film member in a thickness direction of the film member, and a surface of the film member includes a passive film.

公开(公告)号：US20210147795A1

公开(公告)日：2021-05-20

申请号：US17094935

申请日：2020-11-11

Applicant: Natsuko IWASHITA ,  Hidekazu YAGINUMA ,  Momoko SHIONOIRI ,  Tatsuya SAMESHIMA ,  Chihiro KUBO ,  Naoki SATOH ,  Takehiro YAMAZAKI

Inventor： Natsuko IWASHITA ,  Hidekazu YAGINUMA ,  Momoko SHIONOIRI ,  Tatsuya SAMESHIMA ,  Chihiro KUBO ,  Naoki SATOH ,  Takehiro YAMAZAKI

IPC: C12N5/00 ,  G01N33/50 ,  G01N21/64

Abstract: An object of the present invention is to provide a layered body of cells that is used in a co-culture technique and that is capable of recognizing as paracrine effect of each cell and detecting the effect with high intensity. The layered body of the present invention is a layered body 1A having a layered structure in which a gel layer 20a containing a hydrogel is disposed between at least two cell layers 10a and 10b containing cells of different types from each other, wherein the hydrogel is a multi-branched polymer hydrogel formed by a reaction of: Liquid A containing a multi-branched polymer A, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more electrophilic functional groups in at least one of a side chain(s) and an end(s); and Liquid B containing a multi-branched polymer B, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more nucleophilic functional groups in at least one of a side chain(s) and an end(s), the concentration of components derived from the multi-branched polymers A and B in the hydrogel is from 0.6 to 8% by weight, and the thickness is from 0.02 mm to 2 mm.