摘要:
A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein and a layer (i-2) that includes a polycationic material and a layer (ii) that is layered on the layer (i) and includes a cell adhesion factor. The layer (ii) is disposed at a position at which the layer (ii) comes into contact with the cells, or the coat layer includes the layer (i) and the layer (i) is disposed at a position at which the layer (i) comes into contact with the cells, and the culture medium includes the cell adhesion factor.
摘要:
An object of the present invention is to provide a layered body of cells that is used in a co-culture technique and that is capable of recognizing as paracrine effect of each cell and detecting the effect with high intensity. The layered body of the present invention is a layered body 1A having a layered structure in which a gel layer 20a containing a hydrogel is disposed between at least two cell layers 10a and 10b containing cells of different types from each other, wherein the hydrogel is a multi-branched polymer hydrogel formed by a reaction of: Liquid A containing a multi-branched polymer A, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more electrophilic functional groups in at least one of a side chain(s) and an end(s); and Liquid B containing a multi-branched polymer B, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more nucleophilic functional groups in at least one of a side chain(s) and an end(s), the concentration of components derived from the multi-branched polymers A and B in the hydrogel is from 0.6 to 8% by weight, and the thickness is from 0.02 mm to 2 mm.
摘要:
A color image forming method, including: forming electrostatic latent image on electrostatic latent image bearing member; developing the image to form visible image with at least two toners containing releasing agent and selected from black, magenta, cyan and yellow toners; transferring the visible image to recording medium; fixing the transferred image thereon with fixing member having no releasing agent on surface thereof; and forming overcoat layer on the fixed image by polymerizing overcoat composition, wherein when lightness—L1, chromaticity—a1 and chromaticity—b1 according to L*a*b* color system of the fixed image formed with the at least two toners and lightness—L2, chromaticity—a2 and chromaticity-b2 of the fixed image after the overcoat composition is dropped at 0.4 mg/cm2 from height of 10 mm above the fixed image and the overcoat composition is removed after 10 seconds have passed are applied to the following formula (1), color difference ΔE* is 3.0 to 30.0: ΔE*=[(a2−a1)2+(b2−b1)2+(L2−L1)2]1/2 (1).
摘要翻译:一种彩色图像形成方法,包括:在静电潜像承载部件上形成静电潜像; 使用至少两个含有脱模剂的调色剂形成可见图像,并选自黑色,品红色,青色和黄色调色剂; 将可见图像传送到记录介质; 在其表面上用没有脱模剂的固定构件将转印图像固定在其上; 并且通过聚合外涂层组合物在固定图像上形成外涂层,其中当由至少两个调色剂形成的固定图像的L * a * b *颜色系统的亮度-L1,色度a1和色度b1, 外涂层组合物的固定图像的L2,色度a2和色度-b2以0.4mg / cm 2从固定图像上方的10mm的高度下降,并且通过10秒后除去外涂层组合物施加到下式 (1),色差Dgr; E *为3.0〜30.0:&Dgr; E * = [(a2-a1)2+(b2-b1)2+(L2-L1)2] 1/2(1)。
摘要:
An image forming method which applies a zinc-containing metal soap to a surface of an intermediate transfer belt including nitrogen on its surface. At least one of the following formulae is satisfied: (1−C/D)×100(%)−(B/A)×100(%)≦10(%) (F/E)×100(%)≦30(%) wherein A and B represent a percentage content of nitrogen at the surface of the intermediate transfer belt before and after image formation on 1,000 sheets, respectively, C represents a percentage content of zinc at the surface of the intermediate transfer belt after the image formation on 1,000 sheets, D represents a percentage content of zinc based on all elements other than hydrogen in the zinc-containing metal soap, and E and F represent a percentage content of nitrogen at the surface of the intermediate transfer belt before and after application of the metal soap thereto for 5 minutes without image formation, respectively.
摘要:
This invention is to develop a production method for a cell culture carrier comprising a hydrogel having high shape retainability, and cell culture carrier production device, and to provide a structure composed of a cell culture carrier produced using the same. The production method comprises a retention step of retaining on a support material a first solution containing a multiple branching polymer comprising polyethylene glycol as the backbone, and having at least one functional group which is one of a nucleophilic functional group and an electrophilic functional group at a side chain(s) and/or a terminal(s), and a gel formation step of forming one or more dot-shaped hydrogels in which a second solution containing a multiple branching polymer comprising polyethylene glycol as the backbone, and having at least one functional group which is the other one of a nucleophilic functional group and an electrophilic functional group at a side chain and/or a terminal, is discharged by a droplet discharge device to land in contact with the first solution retained on the support material.
摘要:
An image forming method including forming an image of a toner including a wax on a recording medium; fixing the toner image using by an oil-less fixing device; and then forming an overcoat layer on the fixed toner image. When a portion of the fixed toner image having the heaviest toner weight is subjected to an ATR FT-IR analysis, a peak area ratio Ab/Aa falls in a range of 3.0 to 7.0 or a peak area ratio Ab′/Aa′ falls in a range of 0.004 to 0.014, wherein Aa represents the area of a peak present in a range of 2896 cm−1 to 2943 cm−1, Ab represents the area of a peak present in a range of 2946 cm−1 to 2979 cm−1, Aa′ represents the area of a peak present in a range of 791 cm−1 to 860 cm−1, and Ab′ represents the area of a peak present in a range of 2834 cm−1 to 2862 cm−1.
摘要:
A cleaning blade for use in an image forming apparatus including a holder, and an elastic blade attached to the holder and in contact with an image bearing member, the elastic blade being impregnated with an acrylate polymer from at least one surface to a depth of from 5 to 100 μm.
摘要:
Provided is a cell tissue producing method including: a cell adhesive portion forming step of forming a cell adhesive portion formed of a cell adhesive material and having a predetermined shape at a predetermined position over a substrate having a cell non-adhesive surface; and a cell locating step of discharging a cell suspension containing at least cells and a cell drying inhibitor onto the cell adhesive portion in the form of a liquid droplet to locate the cells.
摘要:
A protective agent block including metal soap; and boron nitride, wherein the weight ratio Mr of boron nitride to the metal soap is calculated from the relationship 1 based on Raman spectrum measured at each of multiple places on the protective agent block and the average of the measured weight ratios Mr ranges from 0.050 to 0.330 with a variation coefficient of 0.075 or less: Mr=(log(ABN/ASP)+1.3588)/7.7374 Relationship 1 where ABN represents a peak area in a range of from a wavenumber of 1377.837 cm−1 to 1354.695 cm−1 and ASP represents a peak area in a range of from a wavenumber of 1481.012 cm−1 to 1420.264 cm−1.
摘要:
A color image forming method, including: forming electrostatic latent image on electrostatic latent image bearing member; developing the image to form visible image with at least two toners containing releasing agent and selected from black, magenta, cyan and yellow toners; transferring the visible image to recording medium; fixing the transferred image thereon with fixing member having no releasing agent on surface thereof; and forming overcoat layer on the fixed image by polymerizing overcoat composition, wherein when lightness-L1, chromaticity-a1 and so chromaticity-b1 according to L*a*b* color system of the fixed image formed with the at least two toners and lightness-L2, chromaticity-a2 and chromaticity-b2 of the fixed image after the overcoat composition is dropped at 0.4 mg/cm2 from height of 10 mm above the fixed image and the overcoat composition is removed after 10 seconds have passed are applied to the following formula (1), color difference ΔE* is 3.0 to 30.0: ΔE*=[(a2−a1)2+(b2−b1)2+(L2−L1)2]1/2 (1).
摘要翻译:一种彩色图像形成方法,包括:在静电潜像承载部件上形成静电潜像; 使用至少两个含有脱模剂的调色剂形成可见图像,并选自黑色,品红色,青色和黄色调色剂; 将可见图像传送到记录介质; 在其表面上用没有脱模剂的固定构件将转印图像固定在其上; 并且通过聚合外涂层组合物在固定图像上形成外涂层,其中当由至少两种调色剂形成的固定图像的L * a * b *颜色系统的亮度-L1,色度a1等色度b1时,亮度和亮度 在外涂层组合物从固定图像上方10mm的高度以0.4mg / cm 2下降后的固定图像的-L2,色度a2和色度-b2经过10秒后除去外涂层组合物 式(1),色差DeltaE *为3.0〜30.0:DeltaE * = [(a2-a1)2+(b2-b1)2+(L2-L1)2] 1/2(1)。