公开(公告)号：US20080127373A1

公开(公告)日：2008-05-29

申请号：US10521244

申请日：2005-01-14

申请人： Shinjiro Ogita ,  Hiroshi Sano ,  Nozomu Koizumi ,  Atsuhiko Shinmyo

发明人： Shinjiro Ogita ,  Hiroshi Sano ,  Nozomu Koizumi ,  Atsuhiko Shinmyo

IPC分类号： A01H5/00

CPC分类号： C12N15/8243 ,  A01H5/00 ,  C12N9/1007

摘要： The invention provides a process for producing a decaffeinated coffee plant by genetic recombination comprising a step for preparing an antisense sequence or an RNAi sequence of a gene coding for an enzyme related to the caffeine biosynthetic pathway and constructing an expression vector for transformation, a step for introducing the obtained expression vector into Agrobacterium, a step for infecting a cell division-activated tissue piece of a coffee plant or a callus or an adventitious embryo induced from a tissue piece of a coffee plant with the Agrobacterium to transform the tissue piece, the callus or the adventitious embryo and a step for obtaining an transformed coffee plant from a transformed tissue piece, a transformed callus or a transformed adventitious embryo so that a coffee plant with low caffeine content can be produced by suppressing expression of a gene coding for an enzyme related to the caffeine biosynthetic pathway by the antisense method or the RNAi method.

摘要翻译： 本发明提供了一种通过遗传重组生产脱咖啡因咖啡植物的方法，包括制备编码与咖啡因生物合成途径相关的酶的基因的反义序列或RNAi序列的步骤，并构建用于转化的表达载体的步骤 将获得的表达载体导入农杆菌中，用于感染咖啡植物或愈伤组织的细胞分裂激活的组织片或由土壤杆菌从咖啡植物的组织块诱导的不定胚以转化组织块，愈伤组织 或不定胚，以及从转化的组织片，转化愈伤组织或转化的不定胚获得转化的咖啡植物的步骤，从而可以通过抑制编码相关酶的基因的表达来产生咖啡因含量低的咖啡植物 通过反义方法或RNAi方法转化为咖啡因生物合成途径。

公开(公告)号：US06734342B2

公开(公告)日：2004-05-11

申请号：US09971020

申请日：2001-10-05

申请人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

发明人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

IPC分类号： C12N1529

CPC分类号： C12N15/8243 ,  C12N9/1007

摘要： A theobromine gene derived from Coffea arabica and the polypeptide it encodes are disclosed. The disclosed polypeptide catalyzes biosynthesis of theobromine using 7-methylxanthine as the substrate. Also disclosed are plants transformed with the theobromine gene as to increase or inhibit biosynthesis of theobromine, methods of producing such plants, and seeds obtained from such plants. Caffeineless coffee may be obtained from the disclosed plants, as theobromine synthase participates in the biosynthesis of caffeine.

摘要翻译： 公开了来自咖啡因的可可碱基因及其编码的多肽。 所公开的多肽使用7-甲基黄嘌呤作为底物催化可可碱的生物合成。 还公开了用可可碱基因转化以增加或抑制可可碱的生物合成的植物，产生这种植物的方法，以及从这种植物获得的种子。 咖啡因咖啡可以从所公开的植物获得，因为可可碱合酶参与咖啡因的生物合成。