公开(公告)号：US06734342B2

公开(公告)日：2004-05-11

申请号：US09971020

申请日：2001-10-05

申请人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

发明人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

IPC分类号： C12N1529

CPC分类号： C12N15/8243 ,  C12N9/1007

摘要： A theobromine gene derived from Coffea arabica and the polypeptide it encodes are disclosed. The disclosed polypeptide catalyzes biosynthesis of theobromine using 7-methylxanthine as the substrate. Also disclosed are plants transformed with the theobromine gene as to increase or inhibit biosynthesis of theobromine, methods of producing such plants, and seeds obtained from such plants. Caffeineless coffee may be obtained from the disclosed plants, as theobromine synthase participates in the biosynthesis of caffeine.

摘要翻译： 公开了来自咖啡因的可可碱基因及其编码的多肽。 所公开的多肽使用7-甲基黄嘌呤作为底物催化可可碱的生物合成。 还公开了用可可碱基因转化以增加或抑制可可碱的生物合成的植物，产生这种植物的方法，以及从这种植物获得的种子。 咖啡因咖啡可以从所公开的植物获得，因为可可碱合酶参与咖啡因的生物合成。

公开(公告)号：US06818756B2

公开(公告)日：2004-11-16

申请号：US09805919

申请日：2001-03-15

申请人： Hiroshi Sano ,  Tomonobu Kusano

发明人： Hiroshi Sano ,  Tomonobu Kusano

IPC分类号： C12N1529

CPC分类号： C07K14/705 ,  C12N15/8273

摘要： A novel gene useful for production of a plant exhibiting resistance to environmental stress is provided. According to this invention, C7 gene, a novel gene encoding a receptor-like protein which is induced in response to injury stress, osmotic pressure stress, salt stress or low-temperature stress, is provided. Moreover, a polypeptide encoded by this gene is provided. A plant exhibiting resistance to environmental stress can be produced by incorporation of this novel gene into the plant.

摘要翻译： 提供了一种用于生产耐环境胁迫的植物的新基因。 根据本发明，提供C7基因，其是编码受损伤应激诱导的受体样蛋白质的新基因，渗透压应力，盐胁迫或低温胁迫。 此外，提供了由该基因编码的多肽。 可以通过将这种新基因并入植物中来产生耐环境胁迫的植物。

公开(公告)号：US06596929B2

公开(公告)日：2003-07-22

申请号：US09293836

申请日：1999-04-19

申请人： Koh Iba ,  Takiko Shimada ,  Tomonobu Kusano

发明人： Koh Iba ,  Takiko Shimada ,  Tomonobu Kusano

IPC分类号： C12N1582

CPC分类号： C12N15/8238 ,  C12N15/8223 ,  C12N15/8237 ,  C12N15/8239

摘要： To develop a promoter inducible in response to injury, this invention provides an injury-inducible element, which may be added to a promoter. The invention provides an injury-inducible element that is a base sequence referred to as nucleotide numbers from −611 to −629 SEQ ID No: 1.

摘要翻译： 为了开发可诱导应答损伤的启动子，本发明提供了可以加入到启动子中的损伤诱导元件。本发明提供了一种损伤诱导因子，其是被称为-611至 - 629 SEQ ID No：1。

公开(公告)号：US06479734B2

公开(公告)日：2002-11-12

申请号：US09192434

申请日：1998-11-16

申请人： Koh Iba ,  Takiko Shimada ,  Tomonobu Kusano

发明人： Koh Iba ,  Takiko Shimada ,  Tomonobu Kusano

IPC分类号： A01H500

CPC分类号： C12N9/0083 ,  C07K14/415 ,  C12N15/8237 ,  C12N15/8247 ,  C12N15/8273

摘要： This invention relates to a DNA fragment comprising a base sequence (a) the base sequence referred to as nucleotide numbers 1-3794 in a sequence number 1 in a sequence list; (b) the base sequence (a) a part of which is deleted or substituted by another base sequence, or to which another base sequence is added. Further, the invention relates to a recombinant DNA and transformed plant including the above fragment. Moreover, the invention relates to fragment developing a promoter activity with a responsive property to low temperatures which comprises a base sequence (c) the base sequence referred to as nucleotide numbers 1-2797 in a sequence number 1 in a sequence list; (d) A part of the base sequence (c) developing a promoter activity with a responsive property to low temperatures; or (e) The base sequence (c) or (d) a part of which is deleted or substituted by another base sequence, or to which another base sequence is added. Further, the invention relates to a recombinant DNA and transformed plant including the above fragment.

摘要翻译： 本发明涉及包含碱基序列的DNA片段（a）序列表中序列号1中称为核苷酸编号1-3794的碱基序列; （b）碱基序列（a）其一部分被另一个碱基序列删除或替代，或加入另一个碱基序列。 此外，本发明涉及包含上述片段的重组DNA和转化植物。 此外，本发明涉及片段开发具有低温响应特性的启动子活性，其包含碱基序列（c）序列表中序列号1中称为核苷酸编号1-2797的碱基序列; （d）碱基序列（c）的一部分产生具有低温响应特性的启动子活性; 或（e）碱基序列（c）或（d），其一部分被另一个碱基序列删除或取代，或加入另一个碱基序列。 此外，本发明涉及包含上述片段的重组DNA和转化植物。

公开(公告)号：US06392125B1

公开(公告)日：2002-05-21

申请号：US09468826

申请日：1999-12-22

申请人： Hiroshi Sano ,  Tomonobu Kusano

发明人： Hiroshi Sano ,  Tomonobu Kusano

IPC分类号： A01H500

CPC分类号： C12N15/8205

摘要： A method of genetic improvement of coffee plants, using technique of molecular bleeding, is disclosed. The method provides a transformant of coffee plants produced from embryogenic calli, using Agrobacterium method.

摘要翻译： 公开了一种使用分子渗流技术进行咖啡植物遗传改良的方法。 该方法提供了使用农杆菌法从胚发生愈伤组织产生的咖啡植物的转化体。

公开(公告)号：US5348888A

公开(公告)日：1994-09-20

申请号：US133347

申请日：1993-10-08

申请人： Toshikazu Shiratori ,  Chihiro Inoue ,  Yoshichika Kitagawa ,  Tomonobu Kusano

发明人： Toshikazu Shiratori ,  Chihiro Inoue ,  Yoshichika Kitagawa ,  Tomonobu Kusano

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12N15/74 ,  C12N15/00

CPC分类号： C12N9/0091 ,  C12N15/74 ,  C12Y116/01001

摘要： Microorganisms of the genus Thiobacillus find utility in the mining industry in such applications as bacterial leaching, the treatment of mine drainage water and the deiron in hydrometallurgy process. This invention discloses a DNA fragment which codes for a mercuric reductase in microorganisms of the genus Thiobacillus and is useful in constructing a new strain of Thiobacillus ferrooxidans capable of fast growth. A recombinant plasmid incorporating the DNA fragment, a shuttle vector plasmid incorporating the DNA fragment, host cells and a method of transforming the host cells are also disclosed.

摘要翻译： 硫杆菌属的微生物在采矿业中可用于诸如细菌浸出，矿井排水的处理和湿法冶金过程中的应用。 本发明公开了一种在硫杆菌属微生物中编码汞还原酶的DNA片段，可用于构建能快速生长的新型硫氧化硫杆菌菌株。 还公开了并入DNA片段的重组质粒，掺入DNA片段的穿梭载体质粒，宿主细胞和转化宿主细胞的方法。