公开(公告)号：US06808926B1

公开(公告)日：2004-10-26

申请号：US09645337

申请日：2000-08-25

申请人： Keqiang Wu ,  Brian L. A. Miki ,  Lining Tian ,  Daniel C. W. Brown

发明人： Keqiang Wu ,  Brian L. A. Miki ,  Lining Tian ,  Daniel C. W. Brown

IPC分类号： C12N1529

CPC分类号： C12N15/829 ,  C12N9/16 ,  C12N15/1079 ,  C12N15/1137 ,  C12N15/63 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8237 ,  C12N15/8241 ,  C12N15/8249 ,  C12N15/8261 ,  Y02A40/146

摘要： Posttranslational modification of histones, in particular acetylation and deacetylation are involved in the regulation of gene expression. Histone deacetylases remove acetyl groups from histone proteins. The present invention is directed to a method of regulating gene expression in a transgenic plant comprising, introducing into a plant a first chimeric nucleotide sequence comprising a first regulatory element in operative association with a coding sequence of interest, and an upstream activating sequence, and a second chimeric nucleotide sequence comprising a second regulatory element in operative association with a nucleotide sequence encoding histone deaceytlase and a nucleotide sequence encoding a DNA binding protein, and growing the transgenic plant. Furthermore, a method for regulating gene expression of an endogenous coding sequence of interest, or modifying a developmental, physiological or biochemical pathway in a plant is provided comprising introducing into a plant a chimeric nucleotide sequence comprising a regulatory element in operative association with a nucleotide sequence encoding histone deaceytlase fused with a nucleotide sequence encoding a DNA binding protein capable of interacting with an endogenous controlling sequence, for example an upstream activating sequence, and growing the transgenic plant. This invention also relates to novel histone deacetylase obtained from plants, to novel chimeric construct comprising these, or other histone deacetylase, and to transgenic plants, plant cells, or seeds comprising these chimeric constructs.

摘要翻译： 组蛋白的翻译后修饰，特别是乙酰化和脱乙酰化参与基因表达的调控。 组蛋白脱乙酰酶从组蛋白中去除乙酰基。 本发明涉及一种调节转基因植物中基因表达的方法，包括：向植物中引入包含与感兴趣的编码序列有效结合的第一调节元件的第一嵌合核苷酸序列和上游激活序列，以及 第二嵌合核苷酸序列，其包含与编码组蛋白脱乙酰酶的核苷酸序列有效连接的第二调节元件和编码DNA结合蛋白的核苷酸序列，以及生长所述转基因植物。 此外，提供了调节植物内源性编码序列的基因表达或修饰植物中的发育，生理或生物化学途径的方法，包括向植物中引入包含与核苷酸序列有效结合的调控元件的嵌合核苷酸序列 编码与编码能够与内源性控制序列（例如上游激活序列）相互作用的DNA结合蛋白的核苷酸序列融合的组蛋白脱乙酰肌氨酸酶，以及生长所述转基因植物。 本发明还涉及从植物获得的新型组蛋白脱乙酰酶，包含这些或其它组蛋白脱乙酰酶的新型嵌合构建体以及包含这些嵌合构建体的转基因植物，植物细胞或种子。

公开(公告)号：US06791012B1

公开(公告)日：2004-09-14

申请号：US09763330

申请日：2001-03-27

申请人： Tseh An Chen ,  Shou-Yi Chen ,  Gen-Yun Zhang ,  Faith C. Belanger

发明人： Tseh An Chen ,  Shou-Yi Chen ,  Gen-Yun Zhang ,  Faith C. Belanger

IPC分类号： C12N1529

CPC分类号： C12N9/0008 ,  C12N15/8243 ,  C12N15/8273

摘要： A transgenic turfgrass plant expressing a betaine aldehyde dehydrogenase-encoding transgene is provided. The transgenic plant displays significantly increased tolerance to salinity than does its non-transgenic equivalents. The plant also displays increased tolerance to drought conditions. The salt- and drought-tolerant transgenic turfgrass may be planted in regions of high salinity, such as seaside, or in regions where irrigation water is scarce.

摘要翻译： 提供了表达编码甜菜碱醛脱氢酶的转基因的转基因草坪草植物。 转基因植物显示比其非转基因等同物显着增加的盐度耐受性。 该植物还表现出对干旱条件的增加的耐受性。 耐盐耐旱转基因草坪草可以种植在高盐度的地区，如海边，或灌溉水稀缺的地区。

公开(公告)号：US06734342B2

公开(公告)日：2004-05-11

申请号：US09971020

申请日：2001-10-05

申请人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

发明人： Hiroshi Sano ,  Tomonobu Kusano ,  Nozomu Koizumi

IPC分类号： C12N1529

CPC分类号： C12N15/8243 ,  C12N9/1007

摘要： A theobromine gene derived from Coffea arabica and the polypeptide it encodes are disclosed. The disclosed polypeptide catalyzes biosynthesis of theobromine using 7-methylxanthine as the substrate. Also disclosed are plants transformed with the theobromine gene as to increase or inhibit biosynthesis of theobromine, methods of producing such plants, and seeds obtained from such plants. Caffeineless coffee may be obtained from the disclosed plants, as theobromine synthase participates in the biosynthesis of caffeine.

摘要翻译： 公开了来自咖啡因的可可碱基因及其编码的多肽。 所公开的多肽使用7-甲基黄嘌呤作为底物催化可可碱的生物合成。 还公开了用可可碱基因转化以增加或抑制可可碱的生物合成的植物，产生这种植物的方法，以及从这种植物获得的种子。 咖啡因咖啡可以从所公开的植物获得，因为可可碱合酶参与咖啡因的生物合成。

公开(公告)号：US06734019B1

公开(公告)日：2004-05-11

申请号：US09529239

申请日：2000-10-27

申请人： Marie-Pascale Doutriaux ,  Andreas Stefan Betzner ,  Georges Freyssinet ,  Pascal Perez

发明人： Marie-Pascale Doutriaux ,  Andreas Stefan Betzner ,  Georges Freyssinet ,  Pascal Perez

IPC分类号： C12N1529

CPC分类号： C07K14/415 ,  C12N15/8241

摘要： The present invention relates to an isolated and purified DNA comprising a nucleotide sequence that encodes a polypeptide functionally involved in the DNA mismatch repair system of a plant.

摘要翻译： 本发明涉及分离和纯化的DNA，其包含编码植物的DNA错配修复系统功能性参与的多肽的核苷酸序列。

公开(公告)号：US06727411B2

公开(公告)日：2004-04-27

申请号：US09727758

申请日：2000-12-04

申请人： Hiroaki Kisaka ,  Takao Kida

发明人： Hiroaki Kisaka ,  Takao Kida

IPC分类号： C12N1529

CPC分类号： C12N9/88 ,  C12N15/8251 ,  C12N15/8253

摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate decarboxylase(GAD) gene is introduced into a plant in anti-sense direction together with an appropriate regulatory sequence, and the expression of GAD gene is supressed.

摘要翻译： 提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法 在该方法中，将谷氨酸脱羧酶（GAD）基因连同适当的调节序列一起引入反义方向的植物中，并且抑制GAD基因的表达。

公开(公告)号：US06710227B1

公开(公告)日：2004-03-23

申请号：US09526597

申请日：2000-03-16

申请人： Dirk Inze ,  Lieven De Veylder ,  Janice De Almeida ,  Isabelle Landrieu

发明人： Dirk Inze ,  Lieven De Veylder ,  Janice De Almeida ,  Isabelle Landrieu

IPC分类号： C12N1529

CPC分类号： C07K14/415 ,  A01N65/08 ,  C12N15/8261 ,  Y02A40/146

摘要： Provided are DNA sequences encoding cyclin-dependent kinase inhibitor(s) as well as to methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Furthermore, regulatory sequences which naturally regulate the expression of the above described DNA sequences are described. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cyclin-dependent kinase inhibitor(s) functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided is a process for disruption plant cell division by interfering in the expression or activity of a cyclin-dependent protein kinase inhibitor using a DNA sequence according to the invention wherein said plant cell is part of a transgenic plant. Further described are diagnostic compositions comprising the aforementioned DNA sequences, proteins, antibodies and regulatory sequences. Methods for the identification of compounds being capable of activating or inhibiting the cyclin-dependent kinase inhibitors are described as well. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies, regulatory sequences and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.

公开(公告)号：US06664447B2

公开(公告)日：2003-12-16

申请号：US09803286

申请日：2001-03-09

申请人： Steven D. Tanksley ,  Sergio H. Brommonschenkel

发明人： Steven D. Tanksley ,  Sergio H. Brommonschenkel

IPC分类号： C12N1529

CPC分类号： C07K14/415 ,  C12N15/8283

摘要： The present invention relates to an isolated plant nucleic acid which confers resistance to Tospoviruses as well as expression systems, host cells, and transgenic plants transformed with such a nucleic acid. Other aspects of the present invention relate to a method of imparting to plants, resistance to Tospoviruses by transforming plants with the nucleic acid of the present invention or to a method of eliciting a hypersensitive response in plants.

摘要翻译： 本发明涉及一种分离的植物核酸，其赋予对流感病毒的抗性以及用这种核酸转化的表达系统，宿主细胞和转基因植物。 本发明的其它方面涉及通过用本发明的核酸转化植物或通过在植物中引发过敏反应的方法赋予植物，抗皮肤病毒的方法。

公开(公告)号：US06653463B1

公开(公告)日：2003-11-25

申请号：US09686332

申请日：2000-10-11

申请人： Ching-San Chen ,  Kuan-Chung Chen ,  Cheng-Chun Kuan ,  Ching-Yu Lin

发明人： Ching-San Chen ,  Kuan-Chung Chen ,  Cheng-Chun Kuan ,  Ching-Yu Lin

IPC分类号： C12N1529

CPC分类号： C07K14/415 ,  C12N15/8282 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention relates to novel nucleic acid and protein sequences from the mung bean Vigna radiata. The nucleic acid sequence, isolated from a bruchid resistant mung bean line, encodes a thionin-like protein with insecticidal properties.

摘要翻译： 本发明涉及来自绿豆Vigna radiata的新型核酸和蛋白质序列。 从Bruchid resistant绿豆系分离的核酸序列编码具有杀虫性质的硫素样蛋白质。

公开(公告)号：US06586252B2

公开(公告)日：2003-07-01

申请号：US09916338

申请日：2001-07-30

申请人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

发明人： Jeong-Gu Kang ,  Pill-Soon Song ,  Chung-Mo Park

IPC分类号： C12N1529

CPC分类号： C12N9/16 ,  C12N15/827

摘要： This invention relates to a nucleic acid molecule encoding the catalytic subunit of a protein phosphatase (PP2AC-JD) that belongs to the PP2A family. The PP2AC-JD interacts with the phytochrome A, a primary photoreceptor in the light signal transduction in plants, in the photoperiodic control of flowering. The present invention also provides the methods and processes for generating transgenic higher plants transformed with said nucleic acid molecule to engineer flowing time of economically important crop plants.

摘要翻译： 本发明涉及编码属于PP2A家族的蛋白质磷酸酶（PP2AC-JD）的催化亚基的核酸分子。 PP2AC-JD与植物色素A（植物光信号转导中的初级感光体）在开花的光周期控制中相互作用。 本发明还提供用于产生用所述核酸分子转化的转基因高等植物以设计经济上重要的作物植物的流动时间的方法和方法。

公开(公告)号：US06441270B1

公开(公告)日：2002-08-27

申请号：US08842445

申请日：1997-04-24

申请人： Philip N. Benfey ,  Laura Di Laurenzio ,  Joanna Wysocka-Diller ,  Jocelyn E. Malamy ,  Leonard Pysh ,  Yrjo Helariutta

发明人： Philip N. Benfey ,  Laura Di Laurenzio ,  Joanna Wysocka-Diller ,  Jocelyn E. Malamy ,  Leonard Pysh ,  Yrjo Helariutta

IPC分类号： C12N1529

CPC分类号： C12N15/8227 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8261 ,  Y02A40/146

摘要： The structure and function of a regulatory gene, SCARECROW (SCR), is described. The SCR gene is expressed specifically in root progenitor tissues of embryos, and in roots and stems of seedlings and plants. SCR expression controls cell division of certain cell types in roots and affects the organization of root and stem tissues, and affects gravitropism of aerial structures. The invention relates to the SCARECROW (SCR) gene, SCR gene products, (including but not limited to transcriptional products such as mRNAs, antisense, and ribozyme molecules, and translational products such the SCR protein, polypeptides, peptides and fusion proteins related thereto), antibodies to SCR gene products, SCR promoters and regulatory regions and the use of the foregoing to improve agronomically valuable plants.

摘要翻译： 描述了调节基因SCARECROW（SCR）的结构和功能。 SCR基因在胚胎的根祖细胞组织，以及幼苗和植物的根和茎中特异性表达。 SCR表达控制根中某些细胞类型的细胞分裂，并影响根和茎组织的组织，并影响空中结构的引力。 本发明涉及SCARECROW（SCR）基因，SCR基因产物（包括但不限于转录产物如mRNA，反义核酸酶和核酶分子，以及与其相关的SCR蛋白，多肽，肽和融合蛋白的翻译产物） ，针对SCR基因产物的抗体，SCR启动子和调控区域以及上述用途来改善农艺有价值的植物。