公开(公告)号：US20110097768A1

公开(公告)日：2011-04-28

申请号：US12226029

申请日：2007-04-05

申请人： Oh-Jin Park ,  Sang-Hyun Lee

发明人： Oh-Jin Park ,  Sang-Hyun Lee

IPC分类号： C12P13/02 ,  C12N1/20 ,  C12N9/80 ,  C12P41/00

CPC分类号： C12N9/78 ,  C12P7/40 ,  C12P41/006 ,  C12R1/01

摘要： The present invention relates to a novel Burkholderia multivorans, an amidase produced from the same, and a method for optical resolution of a racemic mixture using the same, and more particularly to a strain Burkholderia multivorans LG 31-3, an amidase having stereoselective substrate specificity, and a method for optical resolution of a racemic mixture using the same. The amidase produced from the novel Burkholderia multivorans LG 31-3 (KCTC 10920BP) according to the present invention can be useful to produce single enantiomer at a high optical purity since the racemic mixture may be easily optically resolved under enzyme reaction conditions of room temperature and normal pressure.

摘要翻译： 本发明涉及一种新颖的伯克霍尔德杆菌多组分，由其产生的酰胺酶，以及使用其的外消旋混合物的光学拆分方法，更具体地涉及一种具有立体选择底物特异性的多克隆多克隆菌株LG 31-3 以及使用该外消旋混合物的光学拆分方法。 由本发明的新型伯克霍尔德杆菌LG 31-3（KCTC 10920BP）生产的酰胺酶可用于以高光学纯度制备单一的对映异构体，因为外消旋混合物可以容易地在室温的酶反应条件下光学拆分， 正常压力。

公开(公告)号：US20090104669A1

公开(公告)日：2009-04-23

申请号：US12224548

申请日：2007-03-02

申请人： Oh-Jin Park ,  Sang-Hyun Lee ,  Sang-Who Lee ,  Goon-Ho Joe ,  Hong-Sun Uh

发明人： Oh-Jin Park ,  Sang-Hyun Lee ,  Sang-Who Lee ,  Goon-Ho Joe ,  Hong-Sun Uh

IPC分类号： C12P17/04

CPC分类号： C07D307/32

摘要： The present invention relates to a method for preparing S-HGB ((S)-3-hydroxy-γ-butyrolactone) using hydrolase, and more particularly to a method for preparing S-HGB in a high purity by hydrolyzing S-BBL ((S)-β-benzoyloxy-γ-butyrolactone) in the presence of hydrolase. According to the present invention, the S-HGB having an optical purity can be obtained in a high yield under simple process conditions without requiring reaction conditions of high pressure and high temperature or complex operating conditions by hydrolyzing S-BBL with hydrolase.

摘要翻译： 本发明涉及使用水解酶制备S-HGB（（S）-3-羟基-γ-丁内酯）的方法，更具体地说，涉及一种通过水解S-BBL（（ S） - 苯甲酰氧基-γ-丁内酯）。 根据本发明，可以在简单的工艺条件下以高产率获得具有光学纯度的S-HGB，而不需要通过用水解酶水解S-BBL的高压和高温或复杂操作条件的反应条件。

公开(公告)号：US08252564B2

公开(公告)日：2012-08-28

申请号：US12226029

申请日：2007-04-05

申请人： Oh-Jin Park ,  Sang-Hyun Lee

发明人： Oh-Jin Park ,  Sang-Hyun Lee

IPC分类号： C12P13/02

CPC分类号： C12N9/78 ,  C12P7/40 ,  C12P41/006 ,  C12R1/01

摘要： The present invention relates to a novel Burkholderia multivorans, an amidase produced from the same, and a method for optical resolution of a racemic mixture using the same, and more particularly to a strain Burkholderia multivorans LG 31-3, an amidase having stereoselective substrate specificity, and a method for optical resolution of a racemic mixture using the same. The amidase produced from the novel Burkholderia multivorans LG 31-3 (KCTC 10920BP) according to the present invention can be useful to produce single enantiomer at a high optical purity since the racemic mixture may be easily optically resolved under enzyme reaction conditions of room temperature and normal pressure.

摘要翻译： 本发明涉及一种新颖的伯克霍尔德杆菌多组分，由其产生的酰胺酶，以及使用其的外消旋混合物的光学拆分方法，更具体地涉及一种具有立体选择底物特异性的多克隆多克隆菌株LG 31-3 以及使用该外消旋混合物的光学拆分方法。 由本发明的新型伯克霍尔德杆菌LG 31-3（KCTC 10920BP）生产的酰胺酶可用于以高光学纯度制备单一的对映异构体，因为外消旋混合物可以容易地在室温的酶反应条件下光学拆分， 正常压力。