公开(公告)号：US08586350B2

公开(公告)日：2013-11-19

申请号：US10597954

申请日：2004-02-12

申请人： Osuman Toujou ,  Masayoshi Ohira ,  Kensuke Okusa ,  Nobuo Seto ,  Masahiro Furuno

发明人： Osuman Toujou ,  Masayoshi Ohira ,  Kensuke Okusa ,  Nobuo Seto ,  Masahiro Furuno

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12Q1/68 ,  C07H21/02 ,  B01D15/08 ,  A23J1/00 ,  C03B23/20

CPC分类号： C07H21/00 ,  C12N15/1006

摘要： A high-purity fragment is obtained by a simple mechanism and method for separating and purifying a nucleic acid, particularly fragment DNA, extremely efficiently and with a high reproducibility, wherein elution with a high-concentration salt is not performed and necessity of elution and purification is eliminated.This mechanism is a mechanism for purifying a nucleic acid, particularly fragment DNA using a monolith structure formed with glass or silica, specifically, an integral porous body having an open structure with pores that communicate the upper end with the lower end, wherein through-pores corresponding to nucleic acid sizes of 35 bp (mer) to 100 Kbp (mer) are provided.

摘要翻译： 通过以非常有效且高再现性分离和纯化核酸，特别是片段DNA的简单的机理和方法获得高纯度片段，其中不进行用高浓度盐洗脱并且需要洗脱和纯化 被淘汰。 该机理是使用由玻璃或二氧化硅形成的整体结构，特别是具有开口结构的整体多孔体来纯化核酸，特别是片段DNA的机理，其具有使上端与下端连通的孔，其中通孔 对应于35bp（mer）至100Kbp（mer）的核酸大小。