公开(公告)号：US20090129980A1

公开(公告)日：2009-05-21

申请号：US11997382

申请日：2006-08-04

申请人： John Lawson ,  Parris Wellsman ,  Sepehr Kiani

发明人： John Lawson ,  Parris Wellsman ,  Sepehr Kiani

IPC分类号： B01J19/00

CPC分类号： B01L3/502715 ,  B01J2219/00522 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01L3/5025 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/163 ,  B01L2400/0406 ,  B01L2400/049 ,  G01N21/05 ,  G01N35/1095 ,  G01N2021/0346

摘要： A multi-channel flow cell can allow for reduced cross-contamination in sample loading and the ability to observe activity within the flow cell once the channels are loaded. A multi-channel flow cell includes a plurality of independently-addressable channels sandwiched between a two substrates. Each of the channels can be coated with a layer that facilitates support-binding of an analyte. Each of the channels terminates on one end in an inlet and on the other end in an outlet. A loading block having inlet ports that match the inlets of the channels can be mated to the inlets of the channels, and an outlet block can be mated to the outlets of the channels. Analytes can be introduced into the channels via the inlet ports of the loading block and are pulled through the channels by capillary action or by vacuum. Once analyte has been introduced into each of the channels, the loading and outlet blocks can be removed and the device turned over Such a flow cell can be used for streamlining the process of reaction and interrogation of biochemical assays at the microfluidic level. Reagents can be introduced into each of the channels of the flow cell for chemical reactions therein, excess reagent being washed out through the channel outlets. Observation of optically-detectable moieties is then conducted. With such a flow cell optical labels associated with incorporation in a sequencing-by-synthesis reaction can be observed.

摘要翻译： 多通道流通池可以允许在样品加载中减少交叉污染，并且一旦加载通道，就能够观察流动池内的活性。 多通道流动池包括夹在两个基板之间的多个可独立寻址的通道。 每个通道可以涂覆有促进分析物的支持结合的层。 每个通道的一端在入口处终止，另一端在出口处终止。 具有与通道的入口匹配的入口端口的装载块可以配合到通道的入口，并且出口块可以配合到通道的出口。 分析物可以通过装载块的入口引入通道，并通过毛细作用或真空被吸引通过通道。 一旦分析物被引入到每个通道中，就可以去除负载和出口嵌段并将器件翻转。这样的流动池可以用于在微流体水平上简化生物化学测定的反应和询问过程。 可以将试剂引入流动池的每个通道中用于其中的化学反应，多余的试剂通过通道出口被洗出。 然后进行光学可检测部分的观察。 通过这样的流动池，可以观察到与逐步合成反应相结合的光学标记。

公开(公告)号：US20080219888A1

公开(公告)日：2008-09-11

申请号：US11928799

申请日：2007-10-30

申请人： John Lawson ,  Parris Wellsman ,  Sepehr Kiani

发明人： John Lawson ,  Parris Wellsman ,  Sepehr Kiani

IPC分类号： B01J19/00

CPC分类号： B01L3/502715 ,  B01J2219/00522 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01L3/5025 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/163 ,  B01L2400/0406 ,  B01L2400/049 ,  G01N21/05 ,  G01N35/1095 ,  G01N2021/0346

摘要： A multi-channel flow cell can allow for reduced cross-contamination in sample loading and the ability to observe activity within the flow cell once the channels are loaded. A multi-channel flow cell includes a plurality of independently-addressable channels sandwiched between a two substrates. Each of the channels can be coated with a layer that facilitates support-binding of an analyte. Each of the channels terminates on one end in an inlet and on the other end in an outlet. A loading block having inlet ports that match the inlets of the channels can be mated to the inlets of the channels, and an outlet block can be mated to the outlets of the channels. Analytes can be introduced into the channels via the inlet ports of the loading block and are pulled through the channels by capillary action or by vacuum. Once analyte has been introduced into each of the channels, the loading and outlet blocks can be removed and the device turned over. Such a flow cell can be used for streamlining the process of reaction and interrogation of biochemical assays at the microfluidic level. Reagents can be introduced into each of the channels of the flow cell for chemical reactions therein, excess reagent being washed out through the channel outlets. Observation of optically-detectable moieties is then conducted. With such a flow cell optical labels associated with incorporation in a sequencing-by-synthesis reaction can be observed.

摘要翻译： 多通道流通池可以允许在样品加载中减少交叉污染，并且一旦加载通道，就能够观察流动池内的活性。 多通道流动池包括夹在两个基板之间的多个可独立寻址的通道。 每个通道可以涂覆有促进分析物的支持结合的层。 每个通道的一端在入口处终止，另一端在出口处终止。 具有与通道的入口匹配的入口端口的装载块可以配合到通道的入口，并且出口块可以配合到通道的出口。 分析物可以通过装载块的入口引入通道，并通过毛细作用或真空被吸引通过通道。 一旦分析物被引入到每个通道中，则可以去除装载和出口块并且将装置翻转。 这样的流动池可以用于在微流控水平下精简生物化学测定的反应和询问过程。 可以将试剂引入流动池的每个通道中用于其中的化学反应，多余的试剂通过通道出口被洗出。 然后进行光学可检测部分的观察。 通过这样的流动池，可以观察到与逐步合成反应相结合的光学标记。