公开(公告)号：US12102083B2

公开(公告)日：2024-10-01

申请号：US17228058

申请日：2021-04-12

申请人： BERKELEY LIGHTS, INC.

发明人： Mark P. White ,  Kevin T. Chapman ,  Andrew W. McFarland ,  Eric D. Hobbs ,  Randall D. Lowe, Jr.

IPC分类号： A01N1/02 ,  B01L3/00 ,  C12M1/00

CPC分类号： A01N1/0284 ,  A01N1/0221 ,  A01N1/0263 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  B01L2200/0668 ,  B01L2300/021 ,  B01L2300/024 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2300/163 ,  B01L2300/18 ,  B01L2300/1894

摘要： A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

公开(公告)号：US12053778B2

公开(公告)日：2024-08-06

申请号：US17529498

申请日：2021-11-18

申请人： LUMICKS CA HOLDING B.V.

发明人： Andrea Candelli ,  Felix Oswald ,  Gerrit Sitters

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01N15/1429 ,  G01N21/03 ,  G01N21/17 ,  G01N29/036 ,  G01N29/22 ,  G01N29/24 ,  G01N29/34 ,  G01N21/64

CPC分类号： B01L3/502761 ,  B01L3/50273 ,  B01L7/00 ,  G01N15/1429 ,  G01N21/0332 ,  G01N29/036 ,  G01N29/222 ,  G01N29/2437 ,  G01N29/2462 ,  G01N29/346 ,  G01N29/348 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2300/163 ,  B01L2300/1822 ,  B01L2400/0436 ,  G01N2021/177 ,  G01N21/6458 ,  G01N2291/014 ,  G01N2291/02466 ,  G01N2291/02475 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/02809 ,  G01N2291/0423 ,  G01N2291/0426

摘要： A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

公开(公告)号：US12031115B2

公开(公告)日：2024-07-09

申请号：US16965878

申请日：2019-01-30

申请人： Katholieke Universiteit Leuven

发明人： Adrian Ranga ,  Sergii Grebeniuk

IPC分类号： C12M1/12 ,  B01L3/00 ,  C12M3/06 ,  C12N5/00

CPC分类号： C12M23/16 ,  B01L3/502715 ,  C12M25/14 ,  C12N5/0075 ,  B01L2300/163 ,  C12N2513/00 ,  C12N2533/54

摘要： The invention relates to devices for the culture of cellular aggregates A comprising a three-dimensional network of interconnected vessels of a biocompatible liquid- and gas-permeable photo polymerised material, wherein the three-dimensional network is connected to a plurality of inlets and a plurality of outlets for the delivery of liquids, and overlayed with a secondary hydrogel network wherein the cellular aggregates are embedded. Herein defined vessels within the three-dimensional network are blocked, thereby defining at least one spatially segregated network within and structurally in contact with the three-dimensional network, wherein the at least one spatially segregated network is connected to a separate inlet and a separate outlet, allowing the supply of a liquid to a subregion within the three-dimensional network.

公开(公告)号：US20230357691A1

公开(公告)日：2023-11-09

申请号：US18346194

申请日：2023-06-30

申请人： CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Donald E. INGBER ,  Dongeun HUH

IPC分类号： C12M3/06 ,  G01N33/50 ,  C12N5/0735 ,  B01L3/00 ,  C12M1/12 ,  C12N5/076 ,  C12N5/0797 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/074 ,  C12M3/00 ,  C12M1/00 ,  C12N5/071 ,  C12N5/0775

CPC分类号： C12M23/16 ,  B01L3/50273 ,  C12M21/08 ,  C12M23/58 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0606 ,  C12N5/061 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0654 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0688 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5005 ,  G01N33/5088 ,  G01N33/5091 ,  B01L3/5027 ,  B01L2200/0663 ,  B01L2300/0854 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0472 ,  B01L2400/0481 ,  B01L2400/0487

摘要： System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

公开(公告)号：US11745184B1

公开(公告)日：2023-09-05

申请号：US17352424

申请日：2021-06-21

申请人： Enzo Biochem, Inc.

发明人： Elazar Rabbani

IPC分类号： B01L3/00 ,  C12M1/32

CPC分类号： B01L3/5085 ,  C12M23/12 ,  B01L2300/0829 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2300/168

摘要： The invention provides reduced background substrates, such as multi-well plates, for use in enzyme-linked immunosorbent assays (ELISAs) and other ligand-binding assays, and methods for making and using the same.

公开(公告)号：US20190151850A1

公开(公告)日：2019-05-23

申请号：US15572128

申请日：2016-05-04

申请人： Illumina, Inc.

发明人： Allen E Eckhardt ,  Rijo Pantoja ,  Sean M Ramirez ,  Petr Capek ,  Edwin Li

IPC分类号： B01L3/00

CPC分类号： B01L3/502784 ,  B01L3/502707 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/161 ,  B01L2300/163 ,  B01L2400/0427

摘要： Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.

公开(公告)号：US20170349871A1

公开(公告)日：2017-12-07

申请号：US15677224

申请日：2017-08-15

申请人： CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Donald E. INGBER ,  Dongeun HUH

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/0789 ,  C12N5/076 ,  C12N5/0797 ,  C12N5/071 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/077 ,  G01N33/50 ,  C12N5/0735

CPC分类号： C12M23/16 ,  B01L3/5027 ,  B01L3/50273 ,  B01L2200/0663 ,  B01L2300/0854 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0472 ,  B01L2400/0481 ,  B01L2400/0487 ,  C12M21/08 ,  C12M23/58 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0606 ,  C12N5/061 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0654 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0688 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5005 ,  G01N33/5088 ,  G01N33/5091

摘要： System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

公开(公告)号：US20170276664A1

公开(公告)日：2017-09-28

申请号：US15619853

申请日：2017-06-12

申请人： The Regents of the University of California

发明人： Michelle Khine ,  Jolie McLane ,  Chun Wu

IPC分类号： G01N33/493 ,  B01L3/00 ,  G01N21/05 ,  G01N21/25 ,  G01N33/68 ,  G01N21/03

CPC分类号： G01N33/493 ,  B01L3/502707 ,  B01L3/502792 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/163 ,  B01L2300/166 ,  B01L2400/0406 ,  B01L2400/088 ,  G01N21/05 ,  G01N21/251 ,  G01N33/68 ,  G01N2021/0325 ,  G01N2021/035

摘要： This disclosure provides a diagnostic system including a detection zone adapted to receive a volume of biological fluid. The detection zone includes a plurality of micro-scale and nano-scale features that render the detection zone superhydrophobic. Analytes (e.g., proteins and/or other molecules) are concentrated when the volume of biological fluid is allowed to evaporate on the detection zone. Concentrating the analytes in the detection zone by evaporation can advantageously increase the sensitivity of detection of the analyte. In various implementations, microfluidic channels can be integrated with the diagnostic system to convey the volume of biological fluid to the detection zone. In various implementations, the microfluidic channels can have a lower hydrophobic characteristic than the surrounding to realize self-driven microfluidic channels that convey the biological fluid to the detection zone without using any external devices.

公开(公告)号：US09757727B2

公开(公告)日：2017-09-12

申请号：US14714452

申请日：2015-05-18

申请人： Massachusetts Institute of Technology

发明人： Scott R. Manalis ,  Robert John Kimmerling ,  Shijie Nigel Chou ,  Vivian C. Hecht

IPC分类号： B01L3/00

CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/12 ,  B01L2300/163 ,  B01L2400/0487 ,  B01L2400/084

摘要： Hydrodynamic Trap Array. The array includes a serpentine bypassing channel including a plurality of trapping pockets disposed therein, the trapping pockets including a ramp entry portion to decrease flow velocity orthogonal to the trapping pocket to increase trapping efficiency. The relative fluid resistances of the trapping pockets and the serpentine bypassing channel are selected such that a slight majority of the flow is diverted to one of the trapping pockets. A pair of microfluidic bypass channels flank the array of traps allowing independent control of upstream and downstream pressures on each side of the array, thereby decoupling flow magnitude in the bypass channels from flow across the trapping pockets.

公开(公告)号：US20170196679A1

公开(公告)日：2017-07-13

申请号：US15360230

申请日：2016-11-23

申请人： RenovaCare Sciences Corp.

发明人： Reinhard Bornemann

IPC分类号： A61F2/10 ,  C12M1/26 ,  A61M11/00 ,  B01L3/02

CPC分类号： A61M11/007 ,  A61M35/00 ,  A61M2205/75 ,  B01L3/0255 ,  B01L2200/0631 ,  B01L2300/0838 ,  B01L2300/163 ,  B01L2400/0487 ,  C12M33/04

摘要： The invention provides a description of a method and a device suitable for producing a cell suspension spray with living cells, and the produced cell preparation, suitable for grafting to a patient. In contrast to other methods, the spraying is performed through a disposable needle which is inserted into a disposable air tube; which provides a cell distribution avoiding spray nozzles. Small suspension droplets are provided instead of cell nebulization. By using medical grade sterile Luer-lock disposables from medical routine praxis, biocompatibility and easy application is addressed. In applying the method and/or in using the device, cells suitable for grafting to a patient are dispersed in a solution and sprayed with the device for distribution over the recipient graft site.