公开(公告)号：US5849537A

公开(公告)日：1998-12-15

申请号：US180524

申请日：1994-01-12

申请人： Matthew Tripp ,  Lance Lusk ,  Thomas Rhodes ,  Nick Huige ,  Edward Kot ,  Etzer Chicoye ,  Michael C. Barney ,  Patricia A. Bower ,  Charles L. Cronan

发明人： Matthew Tripp ,  Lance Lusk ,  Thomas Rhodes ,  Nick Huige ,  Edward Kot ,  Etzer Chicoye ,  Michael C. Barney ,  Patricia A. Bower ,  Charles L. Cronan

IPC分类号： C07K14/46 ,  C12N15/81 ,  C12P21/02 ,  C12N1/19

CPC分类号： C07K14/461 ,  C12N15/81

摘要： Yeast is genetically engineered by transformation with an expression vector containing a natural yeast secretion signal sequence combined appropriately with a chemically synthesized gene encoding antifreeze protein resulting in the expression, proper processing, and secretion of antifreeze protein which is heterologous to yeast in recoverable amounts. Disclosed are DNA sequences comprising structural genes encoding peptides having amino acid sequences with the biochemical or physiochemical properties of antifreeze protein and a method of combining the antifreeze protein gene sequences with appropriate expression vectors.

摘要翻译： 酵母经转基因技术转化，其中含有天然酵母分泌信号序列的表达载体，该序列与编码抗冻蛋白的化学合成基因适当组合，导致可回收量与酵母异源的抗冻蛋白的表达，适当加工和分泌。 公开了包含编码具有具有抗冻蛋白的生物化学或物理化学性质的氨基酸序列的肽的结构基因的DNA序列和将抗冻蛋白基因序列与合适的表达载体组合的方法。

公开(公告)号：US5514576A

公开(公告)日：1996-05-07

申请号：US365189

申请日：1994-12-28

申请人： Patricia A. Bower

发明人： Patricia A. Bower

IPC分类号： G01N30/26 ,  B01D15/08 ,  C07K14/39 ,  C12N1/19 ,  C12N1/21 ,  C12N9/44 ,  C12N15/09 ,  C12N15/56 ,  C12R1/865 ,  G01N30/60 ,  G01N30/88 ,  C12N15/75 ,  C12N15/81

CPC分类号： C07K14/39 ,  C12N9/2457 ,  C12Y302/01041 ,  G01N30/6004 ,  G01N30/88 ,  C07K2319/00 ,  C07K2319/02 ,  G01N2030/8881 ,  G01N30/6034 ,  G01N30/6047

摘要： A method is disclosed for the expression of an active pullulanase enzyme in a microorganism host. In one aspect, a DNA construct contains a sequence encoding the pullulanase enzyme, except for the nucleotides necessary to encode the first two amino acids in mature pullulanase, and regulating sequences allowing expression of the coding sequence in a microorganism host. An advantageous DNA construct contains regulatory sequences permitting expression of the pullulanase in a yeast cell.

摘要翻译： 公开了在微生物宿主中表达活性支链淀粉酶的方法。 一方面，DNA构建体含有编码支链淀粉酶的序列，除了在成熟支链淀粉酶中编码前两个氨基酸所必需的核苷酸以及允许编码序列在微生物宿主中表达的调节序列。 有利的DNA构建体含有允许在酵母细胞中表达支链淀粉酶的调节序列。