公开(公告)号：US20130312127A1

公开(公告)日：2013-11-21

申请号：US13982418

申请日：2011-12-02

申请人： Ten-Tsao Wong ,  Paul Collodi

发明人： Ten-Tsao Wong ,  Paul Collodi

IPC分类号： C12N15/85

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/522 ,  C07K14/5415

摘要： Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1 a or Lif and disruption of the Sdf-1 a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1 a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1 a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.

摘要翻译： 已经开发出通过诱导Sdf-1α的靶向表达或者在鱼胚胎中产生Sdf-1梯度或Lif信号通路的Lif和破坏来破坏鱼胚胎中生殖细胞迁移和发育的方法。 已经产生了含有编码Sdf-1a或Lif的基因和编码用于靶向表达Sdf-1a或Lif的信号序列的基因的质粒构建体。 质粒将被给予鱼或鱼群，以高达100％的效力对人群进行再生灭菌。 本发明的转基因鱼在遗传上污染野生鱼群体的生殖能力不足。

公开(公告)号：US20060242726A1

公开(公告)日：2006-10-26

申请号：US10538820

申请日：2003-12-12

申请人： Paul Collodi ,  Lianchun Fan

发明人： Paul Collodi ,  Lianchun Fan

IPC分类号： A01K67/027 ,  C12N15/09 ,  C12N5/06

CPC分类号： C12N15/8509 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2227/40 ,  C12N15/902

摘要： The present invention includes methods for integrating a modifying polynucleotide into a target polynucleotide in a cell, or instance, a fish cell or a mouse cell, and methods for making chimeric animals, heterozygous animals, and homozygous animals that are chimeric, heterozygous, or homozygous, respectively, for a modifying polynucleotide integrated into a target polynucleotide. The present invention also includes vectors and methods of using the vectors for integrating a modifying polynucleotide into a target polynucleotide in a cell, for instance, a fish cell or a mouse cell.

摘要翻译： 本发明包括用于将修饰多核苷酸整合到细胞或例如鱼细胞或小鼠细胞中的靶多核苷酸的方法，以及用于制备嵌合，杂合或纯合的嵌合动物，杂合动物和纯合动物的方法 分别用于整合到靶多核苷酸中的修饰多核苷酸。 本发明还包括载体和使用载体将修饰多核苷酸整合到细胞例如鱼细胞或小鼠细胞中的靶多核苷酸中的载体和方法。

公开(公告)号：US09534235B2

公开(公告)日：2017-01-03

申请号：US13982418

申请日：2011-12-02

申请人： Ten-Tsao Wong ,  Paul Collodi

发明人： Ten-Tsao Wong ,  Paul Collodi

IPC分类号： A01K67/00 ,  A01K67/033 ,  C12N15/85 ,  A01K67/027 ,  C07K14/52 ,  C07K14/54

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/052 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  A01K2267/0393 ,  C07K14/522 ,  C07K14/5415

摘要： Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1a or Lif and disruption of the Sdf-1a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.

摘要翻译： 已经开发出通过诱导Sdf-1a或Lif的靶向表达破坏生殖细胞迁移和发育的方法，并且在鱼胚中破坏了Sdf-1a梯度或Lif信号通路。 已经产生了含有编码Sdf-1a或Lif的基因和编码用于靶向表达Sdf-1a或Lif的信号序列的基因的质粒构建体。 质粒将被给予鱼或鱼群，以高达100％的效力对人群进行再生灭菌。 本发明的转基因鱼在遗传上污染野生鱼群体的生殖能力不足。