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公开(公告)号：US08222223B2
公开(公告)日：2012-07-17
申请号：US12876735
申请日：2010-09-07
IPC分类号： A61K48/00
摘要： A method of treating biocells includes the steps of: a. providing biocells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, thereby putting the biocells in a catabolic state during which catabolic metabolic functions predominate over anabolic metabolic functions; and c. obtaining at least one product produced by the biocells during the catabolic state. In another embodiment, the method includes the steps of: a. providing biocells that are mammalian cells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, the reparable cell wall damage comprising openings that allow increased passage of materials through the cells walls; and c. inserting foreign DNA through the openings into the biocells.
摘要翻译： 一种处理生物细胞的方法包括以下步骤：a。 提供生物细胞; b。 将至少一个应激源施加到生物细胞上，足以引起对生物细胞的非致死和可修复的细胞壁损伤，从而使生物细胞处于分解代谢状态，其中分解代谢功能占统治于代谢功能; 和c。 在分解代谢状态下获得由生物细胞产生的至少一种产物。 在另一个实施例中，该方法包括以下步骤：a。 提供哺乳动物细胞的生物细胞; b。 将至少一个应激源施加到足以引起对生物细胞的非致死和可修复的细胞壁损伤的生物细胞，所述可修复细胞壁损伤包括允许材料通过细胞壁的增加的通道的开口; 和c。 将外来DNA通过开口插入生物细胞。
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公开(公告)号：US07790427B1
公开(公告)日：2010-09-07
申请号：US11523171
申请日：2006-09-19
IPC分类号： C12N13/00
摘要： A method of treating biocells includes the steps of: a. providing biocells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, thereby putting the biocells in a catabolic state during which catabolic metabolic functions predominate over anabolic metabolic functions; and c. obtaining at least one product produced by the biocells during the catabolic state. In another embodiment, the method includes the steps of: a. providing biocells that are mammalian cells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, the reparable cell wall damage comprising openings that allow increased passage of materials through the cells walls; and c. inserting foreign DNA through the openings into the biocells.
摘要翻译： 一种处理生物细胞的方法包括以下步骤：a。 提供生物细胞; b。 将至少一个应激源施加到生物细胞上，足以引起对生物细胞的非致死和可修复的细胞壁损伤，从而使生物细胞处于分解代谢状态，其中分解代谢功能占统治于代谢功能; 和c。 在分解代谢状态下获得由生物细胞产生的至少一种产物。 在另一个实施例中，该方法包括以下步骤：a。 提供哺乳动物细胞的生物细胞; b。 将至少一个应激源施加到足以引起对生物细胞的非致死和可修复的细胞壁损伤的生物细胞，所述可修复细胞壁损伤包括允许材料通过细胞壁的增加的通道的开口; 和c。 将外来DNA通过开口插入生物细胞。
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公开(公告)号：US20140023555A1
公开(公告)日：2014-01-23
申请号：US14008071
申请日：2012-03-30
IPC分类号： A62D3/37
CPC分类号： A62D3/37 , A62D2101/24 , A62D2101/43 , C23C22/83
摘要： The present invention includes formulations and methods to reduce Cr(VI) contamination, in which the formulation comprises (1) a reactive reducing agent comprising at least one reducing chemical capable of reducing Cr(VI) to Cr(III); and (b) one or more solvents. Moreover, the present invention includes formulations to reduce Cr(VI) within the coating, and Cr(VI) reducing kits with at least one color reference tool for evaluating the process and/or completion of the Cr(VI) reduction.
摘要翻译： 本发明包括减少Cr（VI）污染的制剂和方法，其中制剂包含（1）包含至少一种能还原Cr（VI）至Cr（III）的还原性化合物的反应性还原剂; 和（b）一种或多种溶剂。 此外，本发明包括用于减少涂层内的Cr（VI）的制剂和具有至少一种颜色参考工具的Cr（VI）还原试剂盒，用于评估Cr（VI）还原过程和/或完成。
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公开(公告)号：US09539456B2
公开(公告)日：2017-01-10
申请号：US14008071
申请日：2012-03-30
IPC分类号： A62D3/36 , A62D3/37 , C23C22/83 , A62D101/24 , A62D101/43
CPC分类号： A62D3/37 , A62D2101/24 , A62D2101/43 , C23C22/83
摘要： The present invention includes formulations and methods to reduce Cr(VI) contamination, in which the formulation comprises (1) a reactive reducing agent comprising at least one reducing chemical capable of reducing Cr(VI) to Cr(III); and (b) one or more solvents. Moreover, the present invention includes formulations to reduce Cr(VI) within the coating, and Cr(VI) reducing kits with at least one color reference tool for evaluating the process and/or completion of the Cr(VI) reduction.
摘要翻译： 本发明包括减少Cr（VI）污染的制剂和方法，其中制剂包含（1）包含至少一种能还原Cr（VI）至Cr（III）的还原性化合物的反应性还原剂; 和（b）一种或多种溶剂。 此外，本发明包括用于减少涂层内的Cr（VI）的制剂和具有至少一种颜色参考工具的Cr（VI）还原试剂盒，用于评估Cr（VI）还原过程和/或完成。
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公开(公告)号：US20110059053A1
公开(公告)日：2011-03-10
申请号：US12876735
申请日：2010-09-07
摘要： A method of treating biocells includes the steps of: a. providing biocells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, thereby putting the biocells in a catabolic state during which catabolic metabolic functions predominate over anabolic metabolic functions; and c. obtaining at least one product produced by the biocells during the catabolic state. In another embodiment, the method includes the steps of: a. providing biocells that are mammalian cells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, the reparable cell wall damage comprising openings that allow increased passage of materials through the cells walls; and c. inserting foreign DNA through the openings into the biocells.
摘要翻译： 一种处理生物细胞的方法包括以下步骤：a。 提供生物细胞; b。 将至少一个应激源施加到生物细胞上，足以引起对生物细胞的非致死和可修复的细胞壁损伤，从而使生物细胞处于分解代谢状态，其中分解代谢功能占统治于代谢功能; 和c。 在分解代谢状态下获得由生物细胞产生的至少一种产物。 在另一个实施例中，该方法包括以下步骤：a。 提供哺乳动物细胞的生物细胞; b。 将至少一个应激源施加到足以引起对生物细胞的非致死和可修复的细胞壁损伤的生物细胞，所述可修复细胞壁损伤包括允许材料通过细胞壁的增加的通道的开口; 和c。 将外来DNA通过开口插入生物细胞。
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