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    Gene expression in plants
    1.
    发明授权
    Gene expression in plants 失效
    植物中的基因表达

    公开(公告)号：US06294711B1

    公开(公告)日：2001-09-25

    申请号：US09363970

    申请日：1999-07-29

    申请人： Frank Meulewaeter ,  Marcus Cornelissen ,  Roel Van Aarssen ,  Piet Soetaert ,  Veronique Gossele

    发明人： Frank Meulewaeter ,  Marcus Cornelissen ,  Roel Van Aarssen ,  Piet Soetaert ,  Veronique Gossele

    IPC分类号： C12N504

    CPC分类号： C12N15/8286 ,  C12N15/8216 ,  Y02A40/162

    摘要： The invention provides chimeric genes comprising a first promoter recognized by DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA comprising a 5′ UTR, an AU-rich heterologous coding sequence, a 3′ UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5′ region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3′ region of gonomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a hoterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.

    摘要翻译： 本发明提供了包含不同于真核RNA聚合酶II的DNA依赖性RNA聚合酶识别的第一启动子的嵌合基因; 编码包含5'UTR，富含AU的异源编码序列，3'UTR的嵌合RNA的DNA区域; 并且任选地由所述RNA聚合酶识别的终止子序列，其中编码嵌合RNA的第一启动子和DNA区域可操作地连接，使得在通过RNA聚合酶转录时，产生未加帽的RNA物种，其包含衍生自所述RNA聚合酶的第一翻译增强序列 5'区域的正链RNA植物病毒的基因组或亚基因组RNA; 编码目标多肽或蛋白质的异源RNA编码序列，优选来自富含AT的基因; 和源自正链RNA植物病毒的农业或亚基因组RNA的3'区域的第二翻译增强序列，其中未加帽的RNA物种能够在植物细胞的细胞质中翻译以产生蛋白质或多肽。 还提供了包含这些嵌合基因的植物细胞和植物，其整合在其核DNA中，由此植物细胞产生对应于所使用的启动子和终止子的RNA聚合酶。 此外，本发明提供了一种生产表达由hoterologous基因编码的蛋白质或多肽的植物的方法，其包括用上述嵌合基因转化植物细胞的核基因组的步骤; 并从转化细胞再生转化的植物。

    Gene expression in plants
    4.
    发明授权
    Gene expression in plants 失效
    植物中的基因表达

    公开(公告)号：US5994526A

    公开(公告)日：1999-11-30

    申请号：US880169

    申请日：1997-06-20

    申请人： Frank Meulewaeter ,  Marcus Cornelissen ,  Roel Van Aarssen ,  Piet Soetaert ,  Veronique Gossele

    发明人： Frank Meulewaeter ,  Marcus Cornelissen ,  Roel Van Aarssen ,  Piet Soetaert ,  Veronique Gossele

    IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  C12N15/64

    CPC分类号： C12N15/8286 ,  C12N15/8216

    摘要： The present invention provides chimeric genes that comprise a first promoter recognized by a DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA which comprises a 5' UTR, an AU-rich heterologous coding sequence, a 3' UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5' region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3' region of genomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided in the invention are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a heterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.

    摘要翻译： 本发明提供包含由不同于真核RNA聚合酶II的DNA依赖性RNA聚合酶识别的第一启动子的嵌合基因; 编码嵌合RNA的DNA区域，其包含5'UTR，富含AU的异源编码序列，3'UTR; 并且任选地由所述RNA聚合酶识别的终止子序列，其中编码嵌合RNA的第一启动子和DNA区域可操作地连接，使得在通过RNA聚合酶转录时，产生未加帽的RNA物种，其包含衍生自所述RNA聚合酶的第一翻译增强序列 5'区域的正链RNA植物病毒的基因组或亚基因组RNA; 编码目标多肽或蛋白质的异源RNA编码序列，优选来自富含AT的基因; 和源自正链RNA植物病毒的基因组或亚基因组RNA的3'区域的第二翻译增强序列，其中未加帽的RNA物种能够在植物细胞的细胞质中翻译以产生蛋白质或多肽。 本发明还提供了植物细胞和包含这些嵌合基因的植物，其整合在其核DNA中，由此植物细胞产生对应于所使用的启动子和终止子的RNA聚合酶。 此外，本发明提供了一种生产表达由异源基因编码的蛋白质或多肽的植物的方法，其包括用上述嵌合基因转化植物细胞的核基因组的步骤; 并从转化细胞再生转化的植物。