公开(公告)号：US06602687B1

公开(公告)日：2003-08-05

申请号：US07883630

申请日：1992-05-08

申请人： Michael Sendtner ,  Kurt Stockli-Rippstein ,  Friedrich Lottspeich ,  Yoshihiro Arakawa ,  Patrick Desmond Carroll ,  Rudolf Georg Gotz ,  Georg W. Kreutzberg ,  Dan B. Lindholm ,  Piotr Masiakowski ,  Vivien Wong ,  Nancy Ip ,  Mark E. Furth ,  Nikos Panayotatos ,  Hans Thoenen

发明人： Michael Sendtner ,  Kurt Stockli-Rippstein ,  Friedrich Lottspeich ,  Yoshihiro Arakawa ,  Patrick Desmond Carroll ,  Rudolf Georg Gotz ,  Georg W. Kreutzberg ,  Dan B. Lindholm ,  Piotr Masiakowski ,  Vivien Wong ,  Nancy Ip ,  Mark E. Furth ,  Nikos Panayotatos ,  Hans Thoenen

IPC分类号： C12N1516

CPC分类号： C07K16/22 ,  A61K38/1825 ,  A61K38/185 ,  C07K14/475 ,  A61K38/18 ,  A61K31/715 ,  A61K2300/00

摘要： The present invention relates to nucleic acid sequences encoding ciliary neurotrophic factor (CNTF) and to the proteins, peptides, and derivatives produced therefrom. In various embodiments of the invention, the nucleic acid sequences, proteins, and peptides of the invention may be used in the treatment of a variety of neurological diseases and disorders, including Alzheimer's disease. In a specific embodiment of the invention, CNTF may be used to support the growth of spinal cord neurons, thereby providing a method of treating spinal cord damage caused by trauma infarction, infection, nutritional deficiency or toxic agents. The present invention also relates to a novel method for producing substantilly pure CNTF. The invention also relates to pharmaceutical compositions comprising effective amounts of CNTF gene products which may be used in the diagnosis and treatment of a variety of neurologial diseases and disorders. The present invention relates to the cloning sequencing and expression of CNTF and provides, for the first time, a means for producing human CNTF utilizing human CNTF-encoding nucleic acid sequences. Furthermore, the CNTF nucleic acid sequences of the invention may be utilized to identify nucleic acid sequences encoding CNTF or CNTF-homologous molecules in a variety of species and tissues.

摘要翻译： 本发明涉及编码睫状神经营养因子（CNTF）的核酸序列以及由其产生的蛋白质，肽和衍生物。 在本发明的各种实施方案中，本发明的核酸序列，蛋白质和肽可用于治疗各种神经疾病和病症，包括阿尔茨海默氏病。 在本发明的一个具体实施方案中，CNTF可用于支持脊髓神经元的生长，从而提供治疗由创伤性梗塞，感染，营养缺乏或毒性剂引起的脊髓损伤的方法。本发明还涉及 本发明还涉及包含有效量的可用于诊断和治疗多种神经系统疾病和病症的CNTF基因产物的药物组合物。本发明涉及克隆测序和表达 的CNTF，并且首次提供了利用人CNTF编码核酸序列生产人CNTF的方法。 此外，本发明的CNTF核酸序列可用于鉴定在各种物种和组织中编码CNTF或CNTF同源分子的核酸序列。

公开(公告)号：US5843749A

公开(公告)日：1998-12-01

申请号：US469537

申请日：1995-06-06

申请人： Peter C. Maisonpierre ,  Piotr Masiakowski ,  George D. Yancopoulos

发明人： Peter C. Maisonpierre ,  Piotr Masiakowski ,  George D. Yancopoulos

IPC分类号： C07K14/71 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/02 ,  G01N33/566 ,  G01N33/573 ,  G01N33/68 ,  C07K14/435

CPC分类号： C12N9/1205 ,  C07K14/71 ,  C12Q1/02 ,  G01N33/566 ,  G01N33/573 ,  G01N33/6896 ,  G01N2333/475

摘要： The present invention provides for novel receptor tyrosine kinases known as Ror-1, Ror-2, Ehk-1 and Ehk-2. The invention also provides for assay systems that may be used to detect and/or measure neurotrophin activity or to identify agents that exhibit neurotrophin-like activity. It is based, at least in part, on the discovery that the trkB proto-oncogene encodes a tyrosine kinase receptor that may serve as a functional binding protein for BDNF and NT-3. The present invention also provides for diagnostic and therapeutic methods based on the interaction between BDNF and/or NT-3 and trkB.

摘要翻译： 本发明提供了称为Ror-1，Ror-2，Ehk-1和Ehk-2的新的受体酪氨酸激酶。 本发明还提供了可用于检测和/或测量神经营养因子活性或鉴定表现出神经营养蛋白样活性的试剂的测定系统。 至少部分基于trkB原癌基因编码可能作为BDNF和NT-3的功能性结合蛋白的酪氨酸激酶受体的发现。 本发明还提供了基于BDNF和/或NT-3和trkB之间相互作用的诊断和治疗方法。