公开(公告)号：US20130330316A1

公开(公告)日：2013-12-12

申请号：US13819702

申请日：2011-09-01

Applicant: Bishwajit Kundu ,  Saurabh Bansal ,  Prashant Mishra

Inventor： Bishwajit Kundu ,  Saurabh Bansal ,  Prashant Mishra

IPC: C12N9/82

CPC classification number: C12N9/82 ,  A23L5/25 ,  A61K38/46 ,  C12Y305/01001

Abstract: The present invention relates to a novel mutant of L-asparaginase enzyme characterized in having high thermostability, pH stability and no glutaminase activity useful for therapeutics and the process of preparing the same. The present invention specifically relates to mutant's MTCC 5580, MTCC 5581 and MTCC 5582 characterized in having higher stability, no glutaminase activity etc., to allow their usage in the form of improved protein therapeutics. A thermostable L-asparaginase from P. furiosus was cloned and expressed in E. coli host. The enzyme was engineered at its active site to create three different mutants based on structural and sequence analysis with a E. coli—derived enzyme homologue. The mutants MTCC 5580, MTCC 5581 and MTCC 5582 were tested for their stability, substrate affinity, optimum pH and temperature of activity and cytotoxicity. Based on the studies, all the three enzymes were found thermostable and with no glutaminase activity as compared to other available enzyme EcA II. MTCC 5579 and the above said three mutants showed the cytotoxicity on the leukemic cell lines. The present study showed that these enzymes are promising candidates for the treatment of leukemia.

Abstract translation: 本发明涉及L-天冬酰胺酶的新颖突变体，其特征在于具有高的热稳定性，pH稳定性和无用于治疗的谷氨酰胺酶活性及其制备方法。 本发明具体涉及突变体的MTCC 5580，MTCC 5581和MTCC 5582，其特征在于具有更高的稳定性，没有谷氨酰胺酶活性等，以使其以改进的蛋白质治疗剂的形式使用。 来自热带弧菌的热稳定性L-天冬酰胺酶在大肠杆菌宿主中克隆并表达。 根据大肠杆菌来源的酶同源物的结构和序列分析，酶在其活性位点工程化以产生三种不同的突变体。 测试突变体MTCC 5580，MTCC 5581和MTCC 5582的稳定性，底物亲和力，最佳pH和活性温度和细胞毒性。 基于这些研究，与其他可用的酶EcA II相比，所有这三种酶都被发现是热稳定的，没有谷氨酰胺酶活性。 MTCC 5579和上述所述三种突变体显示对白血病细胞系的细胞毒性。 本研究表明，这些酶是治疗白血病的有希望的候选药物。

公开(公告)号：US09020122B2

公开(公告)日：2015-04-28

申请号：US12783277

申请日：2010-05-19

Applicant: Prashant Mishra ,  Barkha Shukla

Inventor： Prashant Mishra ,  Barkha Shukla

IPC: H04M3/42 ,  H04M1/64 ,  H04M3/22 ,  H04M15/00 ,  H04M15/06

CPC classification number: H04M3/2218 ,  H04M3/42365 ,  H04M15/00 ,  H04M15/06 ,  H04M15/44 ,  H04M15/58

Abstract: Systems and methods for determining and providing call disposition information for outgoing calls are provided. More particularly, the disposition of a call placed from a communication device is determined, and associated with a call log for that communication device. The call disposition information is displayed or otherwise output to a user when the call log is accessed by the user. The call disposition information can be determined by a call disposition application running on a communication server that provides the call disposition information to a communication device for display by that communication device.

Abstract translation: 提供了用于确定和提供用于呼出呼叫的呼叫处理信息的系统和方法。 更具体地，确定从通信设备发出的呼叫的配置，并且与该通信设备的呼叫记录相关联。 当用户访问呼叫日志时，呼叫处理信息被显示或以其他方式输出给用户。 呼叫处理信息可以由在通信服务器上运行的呼叫处理应用来确定，该通信服务器向呼叫处理信息提供通信设备以供该通信设备显示。

公开(公告)号：US08266062B2

公开(公告)日：2012-09-11

申请号：US11769210

申请日：2007-06-27

Applicant: Prashant Mishra

Inventor： Prashant Mishra

IPC: G06F21/00

CPC classification number: H04L9/3236 ,  H04L2209/56

Abstract: A system and method are disclosed relating to authenticating software licenses associated with a software product. During an installation process, a purchaser of a software product may contact a vendor representative via a telephone connection and verbally relay a data packet, which may be an installation ID. The vendor server creates a reversible hash from the data packet, a known seed value and additional information relating to the software product or license. The server encrypts the hash to generate a confirmation ID, and sends the confirmation ID to the software product purchaser.

Abstract translation: 公开了关于认证与软件产品相关联的软件许可的系统和方法。 在安装过程中，软件产品的购买者可以通过电话连接与供应商代表联系，并口头中继可以是安装ID的数据包。 供应商服务器从数据包创建可逆散列，已知种子值和与软件产品或许可证相关的附加信息。 服务器加密哈希生成确认ID，并将确认ID发送给软件产品购买者。

公开(公告)号：US10041137B2

公开(公告)日：2018-08-07

申请号：US11581979

申请日：2006-10-17

Applicant: Carol M. Kinoshita ,  Prashant Mishra ,  Marianne Borowski ,  Peter Francis Daniel

Inventor： Carol M. Kinoshita ,  Prashant Mishra ,  Marianne Borowski ,  Peter Francis Daniel

IPC: A61K38/47 ,  C12P21/00 ,  C12N9/24

Abstract: The invention features a method of producing a high mannose glucocerebrosidase (hmGCB) which includes: providing a cell which is capable of expressing glucocerebrosidase (GCB), and allowing production of GCB having a precursor oligosaccharide under conditions which prevent the removal of at least one mannose residue distal to the pentasaccharide core of the precursor oligosaccharide of GCB, to thereby produce an hmGCB preparation. Preferably, the condition which prevents the removal of at least one mannose residue distal to the pentasaccharide core is inhibition of a class 1 processing mannosidase and/or a class 2 processing mannosidase. The invention also features an hmGCB preparation and methods of using an hmGCB preparation.

公开(公告)号：US20140304663A1

公开(公告)日：2014-10-09

申请号：US13857635

申请日：2013-04-05

Applicant: Prashant Mishra

Inventor： Prashant Mishra

IPC: G06F3/01

CPC classification number: G06F3/017 ,  H04M1/72519 ,  H04M2250/12 ,  H04M2250/64

Abstract: The instant application discloses, among other things, techniques to allow simplified Gesture Interface, which may provide a consistent, easy-to-remember interface for performing various actions with a portable device, including, but not limited to, sharing files, data, and information, winking, waving, pointing, picking up, and dropping.

Abstract translation: 本应用尤其公开了允许简化的手势接口的技术，其可以提供一致的易于记忆的界面，用于使用便携式设备执行各种动作，包括但不限于共享文件，数据和 信息，眨眼，挥舞，指向，拾起和下降。

公开(公告)号：US09322008B2

公开(公告)日：2016-04-26

申请号：US13819702

申请日：2011-09-01

Applicant: Bishwajit Kundu ,  Saurabh Bansal ,  Prashant Mishra

Inventor： Bishwajit Kundu ,  Saurabh Bansal ,  Prashant Mishra

IPC: C12N9/82 ,  A61K38/46 ,  A23L1/015

CPC classification number: C12N9/82 ,  A23L5/25 ,  A61K38/46 ,  C12Y305/01001

Abstract: The present invention relates to a mutant of L-asparaginase enzyme characterized in having high thermostability, pH stability and no glutaminase activity useful for therapeutics and the process of preparing the same. The present invention specifically relates to mutants MTCC 5580, MTCC 5581 and MTCC 5582 characterized in having higher stability, no glutaminase activity etc., to allow their usage in the form of improved protein therapeutics.

Abstract translation: 本发明涉及L-天冬酰胺酶的突变体，其特征在于具有高的热稳定性，pH稳定性和无用于治疗的谷氨酰胺酶活性及其制备方法。 本发明具体涉及突变体MTCC 5580，MTCC 5581和MTCC 5582，其特征在于具有较高的稳定性，无谷氨酰胺酶活性等，以使其以改进的蛋白质治疗剂的形式使用。

公开(公告)号：US20140115503A1

公开(公告)日：2014-04-24

申请号：US13658633

申请日：2012-10-23

Applicant: Prashant Mishra

Inventor： Prashant Mishra

IPC: G06F3/048

CPC classification number: G06F8/38

Abstract: The instant application discloses, among other things, techniques to allow simplified user interface element-based development, allowing users unskilled in conventional programming languages to produce applications by setting attributes, layout, and behaviors for user interface element.

Abstract translation: 本申请尤其公开了允许简化的基于用户界面元素的简化开发的技术，允许不熟练的常规编程语言的用户通过为用户界面元素设置属性，布局和行为来产生应用。

公开(公告)号：US20110286589A1

公开(公告)日：2011-11-24

申请号：US12783277

申请日：2010-05-19

Applicant: Prashant Mishra ,  Barkha Shukla

Inventor： Prashant Mishra ,  Barkha Shukla

IPC: H04M11/00 ,  H04M3/42

CPC classification number: H04M3/2218 ,  H04M3/42365 ,  H04M15/00 ,  H04M15/06 ,  H04M15/44 ,  H04M15/58

Abstract: Systems and methods for determining and providing call disposition information for outgoing calls are provided. More particularly, the disposition of a call placed from a communication device is determined, and associated with a call log for that communication device. The call disposition information is displayed or otherwise output to a user when the call log is accessed by the user. The call disposition information can be determined by a call disposition application running on a communication server that provides the call disposition information to a communication device for display by that communication device.

Abstract translation: 提供了用于确定和提供用于呼出呼叫的呼叫处理信息的系统和方法。 更具体地，确定从通信设备发出的呼叫的配置，并且与该通信设备的呼叫记录相关联。 当用户访问呼叫日志时，呼叫处理信息被显示或以其他方式输出给用户。 呼叫处理信息可以由在通信服务器上运行的呼叫处理应用来确定，该通信服务器向呼叫处理信息提供通信设备以供该通信设备显示。

公开(公告)号：US20090006260A1

公开(公告)日：2009-01-01

申请号：US11769210

申请日：2007-06-27

Applicant: Prashant Mishra

Inventor： Prashant Mishra

IPC: G06Q10/00 ,  H04L9/00

CPC classification number: H04L9/3236 ,  H04L2209/56

Abstract: A system and method are disclosed relating to authenticating software licenses associated with a software product. During an installation process, a purchaser of a software product may contact a vendor representative via a telephone connection and verbally relay a data packet, which may be an installation ID. The vendor server creates a reversible hash from the data packet, a known seed value and additional information relating to the software product or license. The server encrypts the hash to generate a confirmation ID, and sends the confirmation ID to the software product purchaser.

Abstract translation: 公开了关于认证与软件产品相关联的软件许可的系统和方法。 在安装过程中，软件产品的购买者可以通过电话连接与供应商代表联系，并口头中继可以是安装ID的数据包。 供应商服务器从数据包创建可逆散列，已知种子值和与软件产品或许可证相关的附加信息。 服务器加密哈希生成确认ID，并将确认ID发送给软件产品购买者。