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1.发明申请METHODS OF USING FET LABELED OLIGONUCLEOTIDES THAT INCLUDE A 3'-5' EXONUCLEASE RESISTANT QUENCHER DOMAIN AND COMPOSITIONS FOR PRACTICING THE SAME 有权使用包含3'-5'外切酶抗性猝灭子域的FET标记的寡核苷酸的方法和用于实施其的组合物公开(公告)号：US20120245334A1
公开(公告)日：2012-09-27
申请号：US12945777
申请日：2010-11-12
申请人： QUIN CHOU , Dragan Spasic
发明人： QUIN CHOU , Dragan Spasic
IPC分类号： C07H21/04 , C07C245/08
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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2.发明授权Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same 有权使用包括3'-5'外切核酸酶抗性猝灭剂结构域的FET标记的寡核苷酸的方法和用于实施它们的组合物公开(公告)号：US08088583B2
公开(公告)日：2012-01-03
申请号：US12786194
申请日：2010-05-24
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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3.发明申请METHODS OF USING FET LABELED OLIGONUCLEOTIDES THAT INCLUDE A 3'-5' EXONUCLEASE RESISTANT QUENCHER DOMAIN AND COMPOSITIONS FOR PRACTICING THE SAME 有权使用包含3'-5'外切酶抗性猝灭子域的FET标记的寡核苷酸的方法和用于实施其的组合物公开(公告)号：US20090081676A1
公开(公告)日：2009-03-26
申请号：US12197739
申请日：2008-08-25
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本发明方法中，引物延伸反应在具有3'→5'核酸外切酶活性的聚合酶存在下进行，并且至少一个FET标记的寡核苷酸探针包括3' - > 5'外切核酸酶抗性猝灭区。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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4.发明授权Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same 有权使用包括3'-5'外切核酸酶抗性猝灭剂结构域的FET标记的寡核苷酸的方法和用于实施它们的组合物公开(公告)号：US08822673B2
公开(公告)日：2014-09-02
申请号：US12945777
申请日：2010-11-12
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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5.发明授权Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same 有权使用包括3'-5'外切核酸酶抗性猝灭剂结构域的FET标记的寡核苷酸的方法和用于实施它们的组合物公开(公告)号：US07754453B2
公开(公告)日：2010-07-13
申请号：US12197739
申请日：2008-08-25
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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6.发明申请Methods of Using FET Labeled Oligonucleotides That Include a 3'-5' Exonuclease Resistant Quencher Domain and Compositions for Practicing the Same 审中-公开使用包含3'-5'外切核酸酶抗性猝灭剂域的FET标记的寡核苷酸的方法和用于实践其的组合物公开(公告)号：US20070231809A1
公开(公告)日：2007-10-04
申请号：US11530915
申请日：2006-09-12
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本发明方法中，引物延伸反应在具有3'→5'核酸外切酶活性的聚合酶存在下进行，并且至少一个FET标记的寡核苷酸探针包括3' - > 5'外切核酸酶抗性猝灭区。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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7.发明授权Methods of using FET labeled oligonucleotides that include a 3′→5′ exonuclease resistant quencher domain and compositions for practicing the same 有权使用包括3' - > 5'外切核酸酶抗性猝灭区域的FET标记的寡核苷酸的方法和用于实施它们的组合物公开(公告)号：US07160997B2
公开(公告)日：2007-01-09
申请号：US10222943
申请日：2002-08-15
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本发明方法中，引物延伸反应在具有3'→5'核酸外切酶活性的聚合酶存在下进行，并且至少一个FET标记的寡核苷酸探针包括3' - > 5'外切核酸酶抗性猝灭区。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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公开(公告)号：US20060088855A1
公开(公告)日：2006-04-27
申请号：US11198924
申请日：2005-08-04
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
CPC分类号： C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
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9.发明授权Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same 有权使用包括3'-5'外切核酸酶抗性猝灭剂结构域的FET标记的寡核苷酸的方法和用于实施它们的组合物公开(公告)号：US06818420B2
公开(公告)日：2004-11-16
申请号：US10087229
申请日：2002-02-27
IPC分类号： C12P1934
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本发明方法中，引物延伸反应在具有3'→5'核酸外切酶活性的聚合酶存在下进行，并且至少一个FET标记的寡核苷酸探针包括3' - > 5'外切核酸酶抗性猝灭区。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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10.发明申请METHODS OF USING FET LABELED OLIGONUCLEOTIDES THAT INCLUDE A 3'-5' EXONUCLEASE RESISTANT QUENCHER DOMAIN AND COMPOSITIONS FOR PRACTICING THE SAME 有权使用包含3'-5'外切酶抗性猝灭子域的FET标记的寡核苷酸的方法和用于实施其的组合物公开(公告)号：US20110020812A1
公开(公告)日：2011-01-27
申请号：US12786194
申请日：2010-05-24
申请人： Quin Chou , Dragan Spasic
发明人： Quin Chou , Dragan Spasic
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/686 , C12Q2565/101 , C12Q2525/125
摘要： Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like.
摘要翻译： 提供了用于检测反应混合物中引物延伸产物的方法和组合物。 在本方法中，在具有3'→5'核酸外切酶活性的聚合酶和至少一种包含3'→5'外切核酸酶抗性猝灭结构域的FET标记寡核苷酸探针的存在下进行引物延伸反应。 还提供了用于实践主题方法的系统和工具包。 本发明可用于各种不同的应用，并且特别适用于基于高保真PCR的反应，包括SNP检测应用，等位基因变异检测应用等。
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