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公开(公告)号:US20090306342A1
公开(公告)日:2009-12-10
申请号:US12158963
申请日:2006-12-29
CPC分类号: G01N33/54393
摘要: A method of binding a target molecule from an analyte containing the target molecule in admixture with at least one non-target molecule, which method comprises exposing the analyte to a substrate comprising first and second surface binding components, wherein the first surface binding component has specific binding affinity with respect to the target molecule but is independently unable to provide effective binding of the target molecule to the substrate, wherein the second surface binding component has non-specific binding affinity with respect to the target molecule and is independently unable to provide effective specific binding of the target molecule to the substrate, and wherein the target molecule is immobilized on the substrate by the combined binding effect of the first and second surface binding components.
摘要翻译: 一种从含有靶分子的分析物与至少一种非靶分子混合的目标分子的方法,该方法包括将分析物暴露于包含第一和第二表面结合组分的基质上,其中第一表面结合组分具有特异性 相对于靶分子的结合亲和力,但是独立地不能提供靶分子与底物的有效结合,其中第二表面结合组分相对于靶分子具有非特异性结合亲和力,并且独立地不能提供有效的特异性 靶分子与底物的结合,并且其中通过第一和第二表面结合组分的组合结合作用将靶分子固定在基质上。
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公开(公告)号:US20120214973A1
公开(公告)日:2012-08-23
申请号:US13410719
申请日:2012-03-02
申请人: Benjamin Ward MUIR , Michaael C. BARDEN , Dennis Brian RYLATT , N. Joe MAEJI , Carmel Judith HILLYARD , Alain-Dominique Jean-Pierre GORSE , Raisa Leonidovna MONTEIRO
发明人: Benjamin Ward MUIR , Michaael C. BARDEN , Dennis Brian RYLATT , N. Joe MAEJI , Carmel Judith HILLYARD , Alain-Dominique Jean-Pierre GORSE , Raisa Leonidovna MONTEIRO
IPC分类号: C07K17/02
CPC分类号: G01N33/553 , C07K17/06 , G01N33/54353 , G01N33/54393
摘要: A method of immobilising a target molecule on a substrate, which comprises exposing the target molecule to the substrate in the presence of a metal complex, wherein the target molecule is an unmodified target molecule, and wherein the metal complex is selected to provide a stable binding interaction between the target molecule and the substrate.
摘要翻译: 一种将靶分子固定在底物上的方法,其包括在金属络合物的存在下将靶分子暴露于底物,其中靶分子是未修饰的靶分子,并且其中选择金属络合物以提供稳定的结合 靶分子与底物之间的相互作用。
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公开(公告)号:US08168445B2
公开(公告)日:2012-05-01
申请号:US11571422
申请日:2005-06-30
申请人: Benjamin Ward Muir , Michael C. Barden , Dennis Brian Rylatt , N. Joe Maeji , Carmel Judith Hillyard , Alain-Dominique Jean-Pierre Gorse , Raisa Leonidovna Monteiro
发明人: Benjamin Ward Muir , Michael C. Barden , Dennis Brian Rylatt , N. Joe Maeji , Carmel Judith Hillyard , Alain-Dominique Jean-Pierre Gorse , Raisa Leonidovna Monteiro
IPC分类号: G01N33/543
CPC分类号: G01N33/553 , C07K17/06 , G01N33/54353 , G01N33/54393
摘要: A method of immobilizing a target molecule on a substrate, which comprises exposing the target molecule to the substrate in the presence of a metal complex, wherein the target molecule is an unmodified target molecule, and wherein the metal complex is selected to provide a stable binding interaction between the target molecule and the substrate.
摘要翻译: 一种将靶分子固定在基底上的方法,其包括在金属络合物的存在下将靶分子暴露于底物,其中靶分子是未修饰的靶分子,并且其中选择金属络合物以提供稳定的结合 靶分子与底物之间的相互作用。
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