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1.发明授权Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods 有权鉴定涉及抗Cry1毒素的毒素结合蛋白，以及相关筛选方法公开(公告)号：US08039229B2
公开(公告)日：2011-10-18
申请号：US10897621
申请日：2004-07-23
IPC分类号： C12Q1/00 , C12Q1/42 , G01N33/53 , G01N33/554 , G01N33/567 , G01N33/569 , A01N25/00
CPC分类号： G01N33/573 , C12Q1/42 , G01N33/5085 , G01N2333/43552 , G01N2333/916 , Y10S435/832 , Y10S435/962 , Y10S435/967 , Y10S435/97
摘要： The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
摘要翻译： 本发明部分地涉及惊人的和意想不到的发现，与对于Cry毒素敏感的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量的碱性磷酸酶（ALP）活性。 这里报道的这个和其他惊人的发现在诸如管理和监测对B.t.的昆虫抗性的发展的领域具有广泛的影响。 毒素 例如，本发明提供了用于检测ALP活性水平的简单且快速的测定（酶或其它），从而监测昆虫对晶体蛋白质昆虫毒素的抗性的发展。 没有事先的动机或建议，使用这种简单而简单的方法来进行阻力监测。
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2.发明授权Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods 有权鉴定涉及抗Cry1毒素的毒素结合蛋白，以及相关筛选方法公开(公告)号：US08802389B2
公开(公告)日：2014-08-12
申请号：US13274646
申请日：2011-10-17
IPC分类号： A01N25/00 , C12N1/20 , C12Q1/00 , C12Q1/02 , C12Q1/42 , G01N33/53 , G01N33/554 , G01N33/567 , G01N33/569
CPC分类号： G01N33/573 , C12Q1/42 , G01N33/5085 , G01N2333/43552 , G01N2333/916 , Y10S435/832 , Y10S435/962 , Y10S435/967 , Y10S435/97
摘要： The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
摘要翻译： 本发明部分地涉及惊人的和意想不到的发现，与对于Cry毒素敏感的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量的碱性磷酸酶（ALP）活性。 这里报道的这个和其他惊人的发现在诸如管理和监测对B.t.的昆虫抗性的发展的领域具有广泛的影响。 毒素 例如，本发明提供了用于检测ALP活性水平的简单且快速的测定（酶或其它），从而监测昆虫对晶体蛋白质昆虫毒素的抗性的发展。 没有事先的动机或建议，使用这种简单而简单的方法来进行阻力监测。
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3.发明申请IDENTIFICATION OF TOXIN-BINDING PROTEIN INVOLVED IN RESISTANCE TO CRY1 TOXINS, AND RELATED SCREENING METHODS 有权涉及抗CRY1毒素的毒素结合蛋白的鉴定及相关筛选方法公开(公告)号：US20120034610A1
公开(公告)日：2012-02-09
申请号：US13274646
申请日：2011-10-17
IPC分类号： C12Q1/68 , G01N33/573 , C12Q1/42
CPC分类号： G01N33/573 , C12Q1/42 , G01N33/5085 , G01N2333/43552 , G01N2333/916 , Y10S435/832 , Y10S435/962 , Y10S435/967 , Y10S435/97
摘要： The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B. t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
摘要翻译： 本发明部分地涉及惊人的和意想不到的发现，与对于Cry毒素敏感的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量的碱性磷酸酶（ALP）活性。 这里报道的这个和其他惊人的发现在诸如管理和监测对B.t的昆虫抗性的发展的领域具有广泛的影响。 毒素 例如，本发明提供了用于检测ALP活性水平的简单且快速的测定（酶或其它），从而监测昆虫对晶体蛋白质昆虫毒素的抗性的发展。 没有事先的动机或建议，使用这种简单而简单的方法来进行阻力监测。
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4.发明申请Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods 有权鉴定涉及抗Cry1毒素的毒素结合蛋白，以及相关筛选方法公开(公告)号：US20050064386A1
公开(公告)日：2005-03-24
申请号：US10897621
申请日：2004-07-23
申请人： Michael Adang , Rebecca McNall , Juan Fuentes
发明人： Michael Adang , Rebecca McNall , Juan Fuentes
IPC分类号： C12N9/00 , C12Q1/00 , C12Q1/42 , G01N33/50 , G01N33/573
CPC分类号： G01N33/573 , C12Q1/42 , G01N33/5085 , G01N2333/43552 , G01N2333/916 , Y10S435/832 , Y10S435/962 , Y10S435/967 , Y10S435/97
摘要： The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase (ALP) activity as compared to insects that are susceptible to Cry toxins. This and other surprising discoveries reported herein have broad implications in areas such as managing and monitoring the development of insect resistance to B.t. toxins. For example, the subject invention provides a simple and fast assay (enzymatic or otherwise) for detecting ALP activity levels and thereby monitoring the development of resistance by insects to crystal protein insect toxins. There was no prior motivation or suggestion to go about resistance monitoring using this simple and easy approach.
摘要翻译： 本发明部分地涉及惊人的和意想不到的发现，与对于Cry毒素敏感的昆虫相比，对苏云金芽孢杆菌Cry毒素具有抗性的昆虫具有可测量的碱性磷酸酶（ALP）活性。 这里报道的这个和其他惊人的发现在诸如管理和监测对B.t.的昆虫抗性的发展的领域具有广泛的影响。 毒素 例如，本发明提供了用于检测ALP活性水平的简单且快速的测定（酶或其它），从而监测昆虫对晶体蛋白质昆虫毒素的抗性的发展。 没有事先的动机或建议，使用这种简单而简单的方法来进行阻力监测。
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