公开(公告)号：US07241602B2

公开(公告)日：2007-07-10

申请号：US10484681

申请日：2002-07-15

申请人： Theodorus Sonke ,  Renate Francisca Tandler ,  Cornelis Gerrit Nijs Korevaar ,  Friso Bernard Jan Van Assema ,  Rudolf Van Der Pol

发明人： Theodorus Sonke ,  Renate Francisca Tandler ,  Cornelis Gerrit Nijs Korevaar ,  Friso Bernard Jan Van Assema ,  Rudolf Van Der Pol

IPC分类号： C12N9/86 ,  C12N9/04 ,  C12N9/10 ,  C12N1/20 ,  C12P21/06 ,  C12P13/04 ,  C07H21/04

CPC分类号： C12N1/38 ,  C12N9/80 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nucleic acid sequence encoding enantioselective amides with an amino acid sequence of SEQ ID NO: 2. The invention also relates to a process for fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50 mg of Zn2+/ml of fermentation medium is fed during the fermentation. The invention also relates to a process for the preparation of enatiomerically enriched carboxylic acid and/or an enatiomerically enriched carboxylic acid amide, in which a mixture of corresponding D- and L-carboxylic acid amides is contacted with a expression product according to the invention in the presence of 0.01 mM–100 mM Zn2+, whereby one of the enantiomers of the carboxylic acid amide is enatiomerically hydrolysed to from corresponding enatiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.

摘要翻译： 本发明涉及编码具有SEQ ID NO：2的氨基酸序列的对映选择性酰胺的核酸序列。本发明还涉及包含发酵培养基中微生物的批次和进料相的发酵方法，其中 微生物表达根据本发明的核酸，并且其中在发酵过程中加入0.5至50mg的Zn 2+ / ml发酵培养基。 本发明还涉及制备富含对映体的羧酸和/或对映体富集的羧酸酰胺的方法，其中相应的D-和L-羧酸酰胺的混合物与根据本发明的表达产物接触 存在0.01mM-100mM Zn 2+，其中羧酸酰胺的一种对映异构体被对映体水解成相应的富含羧酸的羧酸，而羧酸酰胺的另一种对映异构体保留 不变