公开(公告)号：US4640785A

公开(公告)日：1987-02-03

申请号：US685801

申请日：1984-12-24

申请人： Richard J. Carroll ,  Albert A. Luderer ,  Ward C. Smith ,  Anthony R. Zine, Jr.

发明人： Richard J. Carroll ,  Albert A. Luderer ,  Ward C. Smith ,  Anthony R. Zine, Jr.

IPC分类号： G01N33/50 ,  G01N33/48 ,  G01N33/49 ,  B01D21/26

CPC分类号： G01N33/491 ,  Y10T436/25375

摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.

摘要翻译： 本发明涉及用于抑制浮力密度的明显变化和/或恢复血液样品中粒细胞白细胞的浮力密度的任何损失的手段，从而确保淋巴细胞和单核细胞分离的质量 来自血液样品中的粒细胞。 本发明包括使血液样品与含有低分子量有机或无机离子物质的高渗流体和/或含有高分子量有机物质的等渗或高渗流体接触，所述高分子量或高渗流体可以含有在其结构中具有亲脂性取代基的有机分子， /或用于血细胞的培养基。 本发明的一个组成部分是利用比重在1.060-1.065g / cm 3之间的凝胶状物质的改进的血液分离管，以显着提高细胞分离的纯度，同时提供可接受的细胞产量。

公开(公告)号：US5906744A

公开(公告)日：1999-05-25

申请号：US925851

申请日：1997-09-09

申请人： Richard J. Carroll ,  Frank A. Augello

发明人： Richard J. Carroll ,  Frank A. Augello

IPC分类号： B01L3/14 ,  B01D21/26

CPC分类号： B01L3/5082

摘要： A collection device for plasma preparation for diagnostic assays. The device comprises a spray-dried anticoagulant formulation on the interior surface of the device and a thixotropic polymeric gel. The device is an improvement over commercially available devices which contain liquid anticoagulant formulations, for use in nucleic acid testing that employ amplifications technologies including, but not limited to, polymerase chain reaction (PCR), branched DNA (bDNA) and nucleic acid sequenced based amplification (NASBA).

摘要翻译： 用于诊断测定的血浆制备的收集装置。 该装置包括在装置的内表面上的喷雾干燥的抗凝剂制剂和触变性聚合物凝胶。 该装置是包含液体抗凝血剂制剂的商业可用装置的改进，用于核酸测试，其使用扩增技术，包括但不限于聚合酶链反应（PCR），支链DNA（bDNA）和基于核酸测序的扩增 （NASBA）。

公开(公告)号：US5494590A

公开(公告)日：1996-02-27

申请号：US897305

申请日：1992-06-11

申请人： Ward C. Smith ,  Richard J. Carroll

发明人： Ward C. Smith ,  Richard J. Carroll

IPC分类号： G01N33/48 ,  G01N33/49 ,  G01N33/50 ,  B01D21/26

CPC分类号： G01N33/5002 ,  G01N33/4905 ,  Y10T436/25375

摘要： An anticoagulant solution of sodium citrate for use in blood chemistry-related techniques and apparatus is disclosed. The anticoagulant solution should include an effective concentration of sodium citrate sufficient for preventing the coagulation of a sample of blood employed in the technique or added to the apparatus. The sodium citrate-based anticoagulant solution should have a pH ranging from above 6.0 to about 8.5 and a sodium citrate concentration preferably ranging from about 0.05M to about 0.2M.

摘要翻译： 公开了一种用于血液化学相关技术和装置中的柠檬酸钠抗凝血剂溶液。 抗凝血剂溶液应包括足够的有效浓度的柠檬酸钠，以防止该技术中使用的血液样品的凝结或添加到该装置中。 柠檬酸钠基抗凝血剂溶液的pH应在6.0至8.5之间，柠檬酸钠浓度优选为约0.05M至约0.2M。