公开(公告)号：US07238519B2

公开(公告)日：2007-07-03

申请号：US11016016

申请日：2004-12-17

申请人： Neal F. Bellet ,  Jeffrey Shindelman ,  Thomas E. Worthy ,  Kimberly Haley ,  Richard K-T Lee ,  Jane Eunhee Kyung ,  Suyue Qian

发明人： Neal F. Bellet ,  Jeffrey Shindelman ,  Thomas E. Worthy ,  Kimberly Haley ,  Richard K-T Lee ,  Jane Eunhee Kyung ,  Suyue Qian

IPC分类号： C12M1/34

CPC分类号： G01N21/03 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/0668 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2035/00108 ,  G01N2035/00128 ,  G01N2035/0436 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81

摘要： An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product, and a detection zone located downstream at which the detectable product can be observed. By controlling movement of the support to its first and second positions, the cassette can effectively handle a variety of samples having different flow rates.

摘要翻译： 公开了用于检测液体体液样品中的分析物的免疫测定盒，装置和方法。 盒具有主体和安装在主体上的支撑件，用于相对于主体移动到第一和第二转移位置。 通过将支撑件移动到其第一转移位置，提供给盒体中的样品阱的样品被转移到盒体上的试剂容器。 这里样品与存储器中的第一试剂组合物反应，有效地形成修饰的样品。 然后通过将支撑件移动到其第二转移位置，将改性样品转移到支撑体上的试剂条。 试剂条具有将样品材料转移到条带上的转移区域，有效与经修饰的样品反应以形成可检测的分析物依赖产物的第二试剂组合物，以及位于下游的检测区域，可检测产物可以 观察到的。 通过控制支撑件到其第一和第二位置的移动，盒可以有效地处理具有不同流速的各种样品。