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公开(公告)号:US20130017577A1
公开(公告)日:2013-01-17
申请号:US13510372
申请日:2010-11-17
摘要: The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译: 本发明提供了一种通过将灌注细胞培养物中产生蛋白质(例如,生长期)的细胞生长至高细胞密度(即,至少高于约40×10 6个细胞/小时)来增加细胞培养物中蛋白质产生的方法, N mL),然后切换到蛋白质生产阶段,其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH(即低于pH7)来澄清来自细胞培养物的蛋白质的方法,并沉降细胞培养物,使得细胞培养物分离形成 上清液层和细胞床层,其中蛋白质在上清液层中。
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公开(公告)号:US08765415B2
公开(公告)日:2014-07-01
申请号:US13510372
申请日:2010-11-17
摘要: The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译: 本发明提供了一种通过将灌注细胞培养物中产生蛋白质(例如,生长期)的细胞生长至高细胞密度(即,至少高于约40×10 6个细胞/小时)来增加细胞培养物中蛋白质产生的方法, N mL),然后切换到蛋白质生产阶段,其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH(即低于pH7)来澄清来自细胞培养物的蛋白质的方法,并沉降细胞培养物,使得细胞培养物分离形成 上清液层和细胞床层,其中蛋白质在上清液层中。
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