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公开(公告)号：US20130017577A1
公开(公告)日：2013-01-17
申请号：US13510372
申请日：2010-11-17
摘要： The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译： 本发明提供了一种通过将灌注细胞培养物中产生蛋白质（例如，生长期）的细胞生长至高细胞密度（即，至少高于约40×10 6个细胞/小时）来增加细胞培养物中蛋白质产生的方法， N mL），然后切换到蛋白质生产阶段，其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH（即低于pH7）来澄清来自细胞培养物的蛋白质的方法，并沉降细胞培养物，使得细胞培养物分离形成 上清液层和细胞床层，其中蛋白质在上清液层中。
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公开(公告)号：US08765415B2
公开(公告)日：2014-07-01
申请号：US13510372
申请日：2010-11-17
摘要： The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译： 本发明提供了一种通过将灌注细胞培养物中产生蛋白质（例如，生长期）的细胞生长至高细胞密度（即，至少高于约40×10 6个细胞/小时）来增加细胞培养物中蛋白质产生的方法， N mL），然后切换到蛋白质生产阶段，其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH（即低于pH7）来澄清来自细胞培养物的蛋白质的方法，并沉降细胞培养物，使得细胞培养物分离形成 上清液层和细胞床层，其中蛋白质在上清液层中。
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公开(公告)号：US06986847B2
公开(公告)日：2006-01-17
申请号：US10436353
申请日：2003-05-12
申请人： Kamalesh K. Sirkar , Robert G. Luo , Yanke Xu , Xiao-Ping Dai
发明人： Kamalesh K. Sirkar , Robert G. Luo , Yanke Xu , Xiao-Ping Dai
CPC分类号： B01J20/28014 , B01D15/12 , B01D15/265 , B01D15/322 , B01D15/325 , B01D15/362 , B01D15/363 , B01D15/3804 , B01D61/00 , B01D63/021 , B01D69/00 , B01D69/125 , B01D2313/40 , C07K1/34
摘要： An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane means disposed in the housing for filtering the bioproducts from the feed solution wherein a portion of the membrane means is coated with a polymeric coating, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.
摘要翻译： 一种用于从饲料溶液中回收生物产物的装置和方法。 在一个实施例中，该装置包括模块壳体，设置在壳体中用于过滤来自进料溶液的生物产物的膜装置，其中膜装置的一部分涂覆有聚合物涂层，以及设置在壳体中用于保持 渗透通过膜的生物产物，其中该装置适于通过洗脱来允许从床中分离和纯化保留的生物产物。
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公开(公告)号：US08357514B2
公开(公告)日：2013-01-22
申请号：US12593132
申请日：2008-03-27
CPC分类号： C12N5/0682 , C12N9/003 , C12N15/85 , C12N2840/10 , C12P21/02
摘要： Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.
摘要翻译： 公开了与重组细胞中编码目的多肽的核酸序列的扩增和表达有关的方法，以及由这些方法产生的细胞系和多肽。 本文公开的方法允许通过使用生物反应器在相对短的时间段内扩增表达目的多肽的细胞系。
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公开(公告)号：US20100178673A1
公开(公告)日：2010-07-15
申请号：US12593132
申请日：2008-03-27
申请人： Alahari Arunakumari , Xiao-Ping Dai
发明人： Alahari Arunakumari , Xiao-Ping Dai
CPC分类号： C12N5/0682 , C12N9/003 , C12N15/85 , C12N2840/10 , C12P21/02
摘要： Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.
摘要翻译： 公开了与重组细胞中编码目的多肽的核酸序列的扩增和表达有关的方法，以及由这些方法产生的细胞系和多肽。 本文公开的方法允许通过使用生物反应器在相对短的时间段内扩增表达目的多肽的细胞系。
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