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公开(公告)号：US08765415B2
公开(公告)日：2014-07-01
申请号：US13510372
申请日：2010-11-17
摘要： The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译： 本发明提供了一种通过将灌注细胞培养物中产生蛋白质（例如，生长期）的细胞生长至高细胞密度（即，至少高于约40×10 6个细胞/小时）来增加细胞培养物中蛋白质产生的方法， N mL），然后切换到蛋白质生产阶段，其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH（即低于pH7）来澄清来自细胞培养物的蛋白质的方法，并沉降细胞培养物，使得细胞培养物分离形成 上清液层和细胞床层，其中蛋白质在上清液层中。
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公开(公告)号：US20130017577A1
公开(公告)日：2013-01-17
申请号：US13510372
申请日：2010-11-17
摘要： The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.
摘要翻译： 本发明提供了一种通过将灌注细胞培养物中产生蛋白质（例如，生长期）的细胞生长至高细胞密度（即，至少高于约40×10 6个细胞/小时）来增加细胞培养物中蛋白质产生的方法， N mL），然后切换到蛋白质生产阶段，其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH（即低于pH7）来澄清来自细胞培养物的蛋白质的方法，并沉降细胞培养物，使得细胞培养物分离形成 上清液层和细胞床层，其中蛋白质在上清液层中。
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3.发明授权Method and apparatus for positioning a wellhead member including an overpull indicator 有权用于定位包括过度指示器的井口构件的方法和装置公开(公告)号：US08689888B2
公开(公告)日：2014-04-08
申请号：US12913553
申请日：2010-10-27
申请人： Sibu Varghese , Ryan Herbel , Dien Nguyen , Javier Garcia
发明人： Sibu Varghese , Ryan Herbel , Dien Nguyen , Javier Garcia
IPC分类号： E21B33/03
摘要： An outer wellhead member has a bore with a first profile portion and an annular recess. A tubular inner wellhead member with a centralizer/overpull ring is lowered into the outer wellhead member. The centralizer/overpull ring is biased to expand outward to engage the bore of the outer wellhead member to center the inner wellhead member within the bore as the inner wellhead member is lowered through the bore. The recess of the outer wellhead member is adapted to receive the centralizer/overpull ring and oppose axial movement of the centralizer/overpull ring to enable an upward test pull of the inner wellhead member.
摘要翻译： 外部井口构件具有带有第一轮廓部分和环形凹部的孔。 具有扶正器/超螺旋环的管状内部井口构件下降到外部井口构件中。 当内部井口构件通过孔下降时，对中器/超负荷环被偏压以向外膨胀以接合外部井口构件的孔，以将内部井口构件定位在孔内。 外部井口构件的凹部适于接收扶正器/超车环，并且与对置器/推杆环的轴向运动相反，以使内部井口构件能够向上测试。
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公开(公告)号：US20120245038A1
公开(公告)日：2012-09-27
申请号：US13491989
申请日：2012-06-08
申请人： John Linton , Karl Yoder , Robert Hess , Leila Hasan , Robert Ellis , Tanya S. Kanigan , Kristine Friesen , Arrin Katz , Colin Brenan , Tom Morrison , Javier Garcia
发明人： John Linton , Karl Yoder , Robert Hess , Leila Hasan , Robert Ellis , Tanya S. Kanigan , Kristine Friesen , Arrin Katz , Colin Brenan , Tom Morrison , Javier Garcia
CPC分类号： C12Q1/6858 , B01L3/5025 , B01L3/5027 , B01L3/508 , B01L3/50853 , B01L3/50857 , B01L7/52 , B01L2200/16 , B01L2300/021 , B01L2300/0609 , B01L2300/0636 , B01L2300/0819 , B01L2300/0829 , B01L2300/16 , B01L2300/1822 , B01L2300/1838 , G01N21/03 , G01N21/15 , G01N21/253 , G01N2021/0346 , G01N2021/056 , G01N2021/158 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： A system for holding at least one of sample and reagent for analysis. The system includes a pair of parallel covers, at least one of which is light transmissive, of which pair a light transmissive cover forms a top, and of which pair the other forms a bottom. A frame is disposed between the covers to define, in relation to the covers, an interior volume. The frame and the covers are associated with one another to form a case, the case being substantially tight to liquids. A microfluidic array is disposed in the interior volume. The array includes a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of through-holes running through the thickness between the surfaces, the through-holes containing at least one of sample and reagent.
摘要翻译： 用于容纳至少一个样品和试剂用于分析的系统。 该系统包括一对平行的盖，其至少一个是透光的，其中一对透光盖形成顶部，并且其中一对形成底部。 框架设置在盖之间，以相对于盖限定内部空间。 框架和盖子彼此相关联以形成壳体，该壳体基本上与液体紧密。 微流体阵列设置在内部体积中。 阵列包括具有一对相对表面的厚度的材料片，以及穿过表面之间的厚度的多个通孔，通孔包含至少一个样品和试剂。
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公开(公告)号：US08221503B2
公开(公告)日：2012-07-17
申请号：US11774406
申请日：2007-07-06
申请人： Javier Garcia , Erik J. Wagner , David J. Krueger
发明人： Javier Garcia , Erik J. Wagner , David J. Krueger
CPC分类号： A61B17/025 , A61B17/0206 , A61B17/1604 , A61B17/1671 , A61B17/88 , A61B2017/0256 , A61B2090/062 , A61F2/28 , A61F2/30965 , A61F2/4455 , A61F2/447 , A61F2/4611 , A61F2002/2835 , A61F2002/30062 , A61F2002/3008 , A61F2002/30331 , A61F2002/30387 , A61F2002/30433 , A61F2002/30492 , A61F2002/305 , A61F2002/30777 , A61F2002/30785 , A61F2002/30789 , A61F2002/3082 , A61F2002/30904 , A61F2002/30924 , A61F2002/30952 , A61F2002/4627 , A61F2210/0004 , A61F2220/0025 , A61F2220/0033 , A61F2220/0041 , A61F2250/0098 , A61F2310/00017 , A61F2310/00023 , A61F2310/00293 , A61F2310/00359 , A61F2310/00616 , A61F2310/00796
摘要： A spinal implant may be made of two or more implant members. In an embodiment, implant members may be joined together by a rotational connection that inhibits separation of the members as well as axial movement of the members relative to each other. Implant members may be coupled together by a pin or pins, adhesive, or other fasteners to inhibit separation and/or rotation of the members relative to each other.
摘要翻译： 脊柱植入物可以由两个或更多个植入物构成。 在一个实施例中，植入物构件可以通过旋转连接连接在一起，该旋转连接阻止构件的分离以及构件相对于彼此的轴向运动。 植入物构件可以通过销或销，粘合剂或其它紧固件联接在一起，以防止构件相对于彼此的分离和/或旋转。
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公开(公告)号：US20120103597A1
公开(公告)日：2012-05-03
申请号：US12913553
申请日：2010-10-27
申请人： Sibu Varghese , Ryan Herbel , Dien Nguyen , Javier Garcia
发明人： Sibu Varghese , Ryan Herbel , Dien Nguyen , Javier Garcia
摘要： An outer wellhead member has a bore with a first profile portion and an annular recess. A tubular inner wellhead member with a centralizer/overpull ring is lowered into the outer wellhead member. The centralizer/overpull ring is biased to expand outward to engage the bore of the outer wellhead member to center the inner wellhead member within the bore as the inner wellhead member is lowered through the bore. The recess of the outer wellhead member is adapted to receive the centralizer/overpull ring and oppose axial movement of the centralizer/overpull ring to enable an upward test pull of the inner wellhead member.
摘要翻译： 外部井口构件具有带有第一轮廓部分和环形凹部的孔。 具有扶正器/超螺旋环的管状内部井口构件下降到外部井口构件中。 当内部井口构件通过孔下降时，对中器/超负荷环被偏压以向外膨胀以接合外部井口构件的孔，以将内部井口构件定位在孔内。 外部井口构件的凹部适于接收扶正器/超车环，并且与对置器/推杆环的轴向运动相反，以使内部井口构件能够向上测试。
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公开(公告)号：US20090062134A1
公开(公告)日：2009-03-05
申请号：US12245027
申请日：2008-10-03
申请人： John Linton , Karl Yoder , Robert Hess , Leila Hasan , Robert Ellis , Tanya S. Kanigan , Kristine Friesen , Arrin Katz , Colin Brenan , Tom Morrison , Javier Garcia
发明人： John Linton , Karl Yoder , Robert Hess , Leila Hasan , Robert Ellis , Tanya S. Kanigan , Kristine Friesen , Arrin Katz , Colin Brenan , Tom Morrison , Javier Garcia
IPC分类号： C40B30/00
CPC分类号： C12Q1/6858 , B01L3/5025 , B01L3/5027 , B01L3/508 , B01L3/50853 , B01L3/50857 , B01L7/52 , B01L2200/16 , B01L2300/021 , B01L2300/0609 , B01L2300/0636 , B01L2300/0819 , B01L2300/0829 , B01L2300/16 , B01L2300/1822 , B01L2300/1838 , G01N21/03 , G01N21/15 , G01N21/253 , G01N2021/0346 , G01N2021/056 , G01N2021/158 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： A method of conducting an assay on a plurality of samples is provided. The method includes the steps of performing an assay at each sample site in a sample array having greater than 100 sample sites simultaneously illuminating each sample site using one or more LEDs, and simultaneously imaging each of the sample sites to produce imaging data pertinent to the optical effect of each site. Each assay provides an optical effect.
摘要翻译： 提供了对多个样品进行测定的方法。 该方法包括以下步骤：在具有大于100个样品位点的样品阵列中的每个样品位置处进行测定，同时使用一个或多个LED照亮每个样品位点，并且同时对每个样品位点成像以产生与光学相关的成像数据 每个网站的效果。 每个测定提供光学效果。
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公开(公告)号：US20050157313A1
公开(公告)日：2005-07-21
申请号：US11081214
申请日：2005-03-16
申请人： David Mendlovic , Boris Glushko , Efraim Goldenberg , Gal Shabtay , Javier Garcia , Leonard Bergstein , Shlomo Eisenbach , Yehuda Miron , Aviram Sariel
发明人： David Mendlovic , Boris Glushko , Efraim Goldenberg , Gal Shabtay , Javier Garcia , Leonard Bergstein , Shlomo Eisenbach , Yehuda Miron , Aviram Sariel
CPC分类号： G06E3/001 , G01B9/02098 , G02B26/06 , G06E3/00
摘要： A shearing generator comprising: an input light source; an image generator that generates two images of the input light source at an output plane, the image generator comprising a beam splitter that splits light from the input source into at least one pair of interfering light waves at an output thereof, and defines different optical paths for the light propagation of the light waves, said optical paths including at least one phase shifting element that provides for a different phase shift for the two paths.
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公开(公告)号：US06677120B2
公开(公告)日：2004-01-13
申请号：US09822903
申请日：2001-03-30
IPC分类号： C12Q168
摘要： The present invention is directed to methods for the preparation of 3′-O and 5′-O-levulinyl nucleosides from common precursors using an enzymatic approach.
摘要翻译： 本发明涉及使用酶法从常用前体制备3'-O和5'-O-左乙酰基核苷的方法。
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公开(公告)号：US08638991B2
公开(公告)日：2014-01-28
申请号：US12670477
申请日：2008-07-21
申请人： Zeev Zalevsky , Javier Garcia
发明人： Zeev Zalevsky , Javier Garcia
IPC分类号： G06K9/00
CPC分类号： G01B9/02094 , A61B5/021 , A61B5/024 , G01B9/02083 , G01H9/002
摘要： A method is presented for imaging an object. The method comprises imaging a coherent speckle pattern propagating from an object, using an imaging system being focused on a plane displaced from the object.
摘要翻译： 呈现一种用于成像对象的方法。 该方法包括使用成像系统聚焦在从物体移位的平面上成像从物体传播的相干散斑图案。
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