公开(公告)号：US20060020119A1

公开(公告)日：2006-01-26

申请号：US11093724

申请日：2005-03-29

申请人： Stephen Grimes ,  Ronald Makishima

发明人： Stephen Grimes ,  Ronald Makishima

IPC分类号： C07K16/26 ,  G01N33/53 ,  C12Q1/68

CPC分类号： C07K16/26 ,  A61K2039/505 ,  C07K2317/34 ,  C07K2317/73 ,  G01N33/577 ,  G01N33/74 ,  G01N2333/595

摘要： The present invention provides monoclonal antibodies (MAbs) selective for the N-termini and C-termini of the gastrin hormone forms, gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly); and the hybridomas that produce these MAbs. Also provided are panels of MAbs useful for the detection and quantitation of gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly). These assays are useful for monitoring a gastrin-mediated disease or condition, or for monitoring the progress of a course of therapy. The invention further provides solid phase assays including immunohistochemical (IHC) and immunofluorescence (IF) assays suitable for detection and visualization of gastrin species in solid samples, such as biopsy samples or tissue slices. Pharmaceutical compositions of the MAbs of the invention are also provided, along with methods of diagnosis, prevention and treatment of gastrin-mediated diseases or conditions. Methods of evaluating a gastrin hormone-blocking treatment are described. The course of a gastrin-mediated disease or condition may be monitored in a patient by means of assay methods provided.

摘要翻译： 本发明提供对胃泌素激素形式的N-末端和C-末端选择性的单克隆抗体（MAb），胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34） 和甘氨酸延长胃泌素-34（G34-Gly）; 和产生这些MAb的杂交瘤。 还提供了可用于检测和定量胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34）和甘氨酸延长胃泌素-34（G34-Gly） 。 这些测定法可用于监测胃泌素介导的疾病或病症，或用于监测治疗过程的进展。 本发明进一步提供固相测定法，包括免疫组织化学（IHC）和免疫荧光（IF）测定法，其适于检测和可视化固体样品例如活检样本或组织切片中的胃泌素种类。 还提供本发明的MAb的药物组合物以及胃泌素介导的疾病或病症的诊断，预防和治疗的方法。 描述了评估胃泌素激素阻断治疗的方法。 胃泌素介导的疾病或病症的过程可以通过提供的测定方法在患者体内监测。

公开(公告)号：US20070066809A1

公开(公告)日：2007-03-22

申请号：US11499261

申请日：2006-08-03

申请人： Stephen Grimes ,  Ronald Makishima

发明人： Stephen Grimes ,  Ronald Makishima

IPC分类号： C07K16/00

CPC分类号： C07K16/26 ,  A61K2039/505 ,  C07K2317/34 ,  C07K2317/73 ,  G01N33/577 ,  G01N33/74 ,  G01N2333/595

摘要： The present invention provides monoclonal antibodies (MAbs) selective for the N-termini and C-termini of the gastrin hormone forms, gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly); and the hybridomas that produce these MAbs. Also provided are panels of MAbs useful for the detection and quantitation of gastrin-17 (G17), glycine-extended gastrin-17 (G17-Gly), gastrin-34 (G34) and glycine-extended gastrin-34 (G34-Gly). These assays are useful for monitoring a gastrin-mediated disease or condition, or for monitoring the progress of a course of therapy. The invention further provides solid phase assays including immunohistochemical (IHC) and immunofluorescence (IF) assays suitable for detection and visualization of gastrin species in solid samples, such as biopsy samples or tissue slices. Pharmaceutical compositions of the MAbs of the invention are also provided, along with methods of diagnosis, prevention and treatment of gastrin-mediated diseases or conditions. Methods of evaluating a gastrin hormone-blocking treatment are described. The course of a gastrin-mediated disease or condition may be monitored in a patient by means of assay methods provided.

摘要翻译： 本发明提供对胃泌素激素形式的N-末端和C-末端选择性的单克隆抗体（MAb），胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34） 和甘氨酸延长胃泌素-34（G34-Gly）; 和产生这些MAb的杂交瘤。 还提供了可用于检测和定量胃泌素-17（G17），甘氨酸延长胃泌素-17（G17-Gly），胃泌素-34（G34）和甘氨酸延长胃泌素-34（G34-Gly） 。 这些测定法可用于监测胃泌素介导的疾病或病症，或用于监测治疗过程的进展。 本发明进一步提供固相测定法，包括免疫组织化学（IHC）和免疫荧光（IF）测定法，其适于检测和可视化固体样品例如活检样本或组织切片中的胃泌素种类。 还提供本发明的MAb的药物组合物以及胃泌素介导的疾病或病症的诊断，预防和治疗的方法。 描述了评估胃泌素激素阻断治疗的方法。 胃泌素介导的疾病或病症的过程可以通过提供的测定方法在患者体内监测。