公开(公告)号：US20130157283A1

公开(公告)日：2013-06-20

申请号：US13522800

申请日：2011-01-19

申请人： Chong Wing Yung ,  Donald E. Ingber ,  Ryan Mcomber Cooper ,  Frank Vollmer ,  Karel Domansky ,  Daniel Christopher Leslie ,  Michael Super

发明人： Chong Wing Yung ,  Donald E. Ingber ,  Ryan Mcomber Cooper ,  Frank Vollmer ,  Karel Domansky ,  Daniel Christopher Leslie ,  Michael Super

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2400/043 ,  G01N33/54306 ,  G01N33/54333 ,  G01N33/54366 ,  G01N35/0098

摘要： A microfluidic device of a diagnostic and detection system includes an inlet port connected by one or more microchannels to an outlet port and includes a capture and visualization chamber (CVC) connected to at least one microchannel. A fluid to be analyzed can be mixed with magnetic microbeads that have an affinity to become bound to target components, such as pathogens in the fluid. The fluid including the magnetically bound target components can be injected through the microfluidic device. Magnetic field gradient, such as provided by permanent or electro-magnets, can be applied to the fluid and the magnetically bound target components flowing through the microfluidic device to cause the magnetically bound target components to migrate into the (CVC) and become separated from the fluid. The magnetically bound target components can be analyzed and tested using various techniques to detect the presence of specific organic and inorganic materials, such as pathogens in bio-fluids and contamination in liquid food sources (e.g. water). The device and method provide a system for rapidly detecting pathogens and contamination in relatively small fluid samples.

摘要翻译： 诊断和检测系统的微流体装置包括通过一个或多个微通道连接到出口的入口端口，并且包括连接到至少一个微通道的捕获和可视化室（CVC）。 要分析的流体可以与具有亲和性的磁性微珠混合，以结合到目标组分，例如流体中的病原体。 包括磁结合的靶组分的流体可以通过微流体装置注入。 磁场梯度，例如由永磁体或电磁铁提供的磁场梯度可以被施加到流过微流体装置的流体和磁结合的目标组分，以使磁结合的靶组分迁移到（CVC）中并与 流体。 可以使用各种技术来分析和测试磁结合的靶组分，以检测特定有机和无机材料（例如生物流体中的病原体和液体食物来源（例如水））中的污染物的存在。 该装置和方法提供用于在相对小的流体样品中快速检测病原体和污染物的系统。