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    POLYMERASE STABILIZATION
    1.
    发明申请
    POLYMERASE STABILIZATION 审中-公开
    聚合物稳定

    公开(公告)号：US20100159528A1

    公开(公告)日：2010-06-24

    申请号：US12601102

    申请日：2008-06-12

    申请人： Yi Liu ,  Ryan Westberry ,  Scott Hamilton

    发明人： Yi Liu ,  Ryan Westberry ,  Scott Hamilton

    IPC分类号： C12P19/34 ,  C12N9/96

    CPC分类号： C12N9/96 ,  C12N9/1252

    摘要： The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.

    摘要翻译： 本发明涉及用于提供不含外源洗涤剂的纯化的热稳定酶，特别是耐热性DNA聚合酶的方法和组合物。 本发明还提供了通过添加一种或多种洗涤剂来提供这种纯化的热稳定性DNA聚合酶以活性形式的测定法的方法。 本发明进一步提供了包含用于各种应用的纯化的热稳定性DNA聚合酶的组合物和试剂盒，包括核酸的扩增和测序。

    Dutp Based Compositions for Reducing Primer-Aggregate Formations During Nucleic Acid Amplification
    2.
    发明申请
    Dutp Based Compositions for Reducing Primer-Aggregate Formations During Nucleic Acid Amplification 有权
    用于减少核酸扩增过程中底物聚集体形成的Dutp基组合物

    公开(公告)号：US20070264633A1

    公开(公告)日：2007-11-15

    申请号：US10588689

    申请日：2005-02-04

    申请人： Ryan Westberry ,  Lars-Erik Peters ,  Jessica Jaclyn Greenlee

    发明人： Ryan Westberry ,  Lars-Erik Peters ,  Jessica Jaclyn Greenlee

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6848 ,  C12Q1/6853 ,  C12Q2527/125 ,  C12Q2525/121 ,  C12Q2525/101

    摘要： Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

    摘要翻译： 提供了增强扩增反应混合物的特异性和灵敏度的方法和组合物。 根据本发明的组合物提供在扩增反应期间减少引物 - 聚集体的形成。 反应混合物包括dNTP，其中一部分dNTP已被非常规核苷酸替代，例如dUTP。 非常规核苷酸浓度通常在标准dNTP之一的浓度的10％至5​​0％之间。 在一些组合物中，非常规核苷酸是dUTP，其代替dNTP混合物中dTTP的约10％至约50％。